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Hemicellulase and its application in preparing chitoligose

A technology of hemicellulase and hemicellulase, applied in the direction of hydrolytic enzymes, etc., can solve the problems of long enzymatic hydrolysis time, low enzymatic activity of chitosan enzyme, high energy consumption, etc., and achieve the effect of improving activity

Inactive Publication Date: 2005-12-07
武汉东湖生物技术发展有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the second issue of "China Marine Medicine" in 1999, the article "Screening of High-yielding Chitosanase Strains and Preliminary Research on Chitosan Degradation Reaction" discussed that the Chinese Academy of Sciences Dalian Chemical Physics and Biochemical Laboratory obtained two rounds of effective ultraviolet mutagenesis. 2 mutant strains with high yield of chitosanase have good stability, and the crude enzyme activity of their fermented liquid reaches 0.2u / ml, although it is 8 times higher than the original preserved mutant strain enzyme activity, and a certain amount Oligochitosan, but its enzyme activity is still low and cannot be industrialized
There are a large number of chitosan degrading enzymes, and the degraded chitosan enzymes generally have problems such as low enzyme activity, many side reactions, long enzymatic hydrolysis time, and high energy consumption.

Method used

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  • Hemicellulase and its application in preparing chitoligose
  • Hemicellulase and its application in preparing chitoligose
  • Hemicellulase and its application in preparing chitoligose

Examples

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Embodiment 1

[0049] Embodiment 1: the preparation of hemicellulase of the present invention

[0050] 1. Inoculate the Hemicellulase A1 strain by streaking method on the plate medium containing Tryptone (Tryptone) 1% yeast extract (Yeast extract) 0.5% sodium chloride (Nacl) 1% and agar 1% , used after culturing at 37°C for 18 hours;

[0051] 2. Use a 1000ml Erlenmeyer shaker flask to pack 100ml of liquid medium containing 10% dextrin, tryptone (Tryptone) 1% yeast extract (Yeast extract) 0.5% Nacl 1%, sterilize on 10 pounds for 20 minutes, and inoculate a Ring, shake (250r / min) on a shaker at 36°C for 18 hours;

[0052] 3. Put 8 liters of liquid medium containing medium dextrin, tryptone, and yeast extract in a 10 liter fermenter, sterilize as above, then connect 100 ml of shake flask seed suspension, and cultivate with aeration and stirring at 36° C. for 24 hours.

[0053] 4. Low-temperature centrifugal separation of enzyme liquid: put the fermentation liquid into the centrifuge barrel of...

Embodiment 2

[0054] Embodiment 2: Utilize hemicellulase provided by the invention to produce chitosan oligosaccharide

[0055] 1. Process flow: chitosan glacial acetic acid solution → tank enzymatic hydrolysis → centrifugation, flocculation, filtration to remove impurities → decolorization, deodorization → ultrafiltration classification → vacuum concentration → spray drying → chitosan oligosaccharides

[0056] 2. Operating procedures: (1) enzymatic hydrolysis process glacial acetic acid is prepared into a 2% solution, add chitosan and heat up and stir to dissolve, the temperature is controlled at 48°C, the volume-to-weight ratio of chitosan and glacial acetic acid solution (W / V ) is 10%. Add the hemicellulose enzymatic solution of the present invention for degradation, the reaction temperature is 48°C-50°C, heat preservation, stirring, time 8 hours; (2) Centrifugal filtration Centrifugal filtration of the reacted enzymolysis solution, rotating speed 3000r / min, time 15 Minutes; (3) floccul...

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Abstract

A hemicellulase is the fermented product of Bacillus pumilus hemicellulase No.A1 (CCTCC M201023) and can be used in preparing chitoligose. A process for preparing the said chitoligose includes such steps as zymolyzing chitosan with the said hemicellulase, centrifugal filtering, flocculating, vacuum concentrating, filtering, decolouring, deodouring, super filtering and classifying and drying. Its advantages are high zymolyzing efficiency and high content of active components in product.

Description

technical field [0001] The invention relates to a hemicellulase and its application in preparing chitosan oligosaccharide. Background technique [0002] Chitin is a natural animal dietary fiber produced and processed from shrimp and crab shells that exist in abundance in rivers, lakes, and seas. Chitosan is a natural polymer compound obtained by removing the acetyl group in chitin molecules under the action of concentrated alkali, and its content in nature is second only to cellulose. Chitin is mainly divided into the following three levels of derivative products based on different processing methods: A. Chitin (chitin), with a molecular weight of more than 1 million, has a strong absorption capacity and is suitable for industrial and environmental applications, but it is insoluble in Acid-base, insoluble in water, difficult to be absorbed by the body. B. Chitosan (chitosan), with a molecular weight of about 100,000, is generally only soluble in o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08C12N9/24
Inventor 吴健
Owner 武汉东湖生物技术发展有限公司
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