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Application of verbenin in medicine for treating alcoholic liver injury

A technology of alcohol-induced liver injury and verbenaside, applied in the field of pharmacotherapy, can solve the problem of ALI without any effective treatment measures, and achieve the effects of protecting cerebral ischemia injury, wide pharmacological effects, and promoting sleep.

Active Publication Date: 2022-08-09
ANHUI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there is no effective treatment for ALI. Therefore, it is of great significance to find liver protection drugs that can reduce tissue damage, promote repair, and prevent chronic fibrosis and liver cancer.

Method used

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  • Application of verbenin in medicine for treating alcoholic liver injury
  • Application of verbenin in medicine for treating alcoholic liver injury
  • Application of verbenin in medicine for treating alcoholic liver injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Effects of verbenaside on general condition and liver of alcohol-induced mice:

[0032] experimental design:

[0033] 36 male C57BL / 6J mice, weighing 20±2 g, were randomly divided into the following 6 groups (n=6): control group (Vehicle), control + verbenaside (50mg / kg) group, alcohol group, alcohol + The verbenaside (12.5mg / kg) group, the alcohol + verbenin (25mg / kg) group and the alcohol + verbenin (50mg / kg) group.

[0034] A mouse model of ALI (Alcohol-induced liver injury) was established by chronic long-term alcohol intake: after one week of adaptive feeding, the mice were randomly divided into 6 groups, 6 mice in each group, and the control group ( Vehicle), control + verbenin (50mg / kg) group, alcohol group, alcohol + verbenin (12.5mg / kg) group, alcohol + verbenin (25mg / kg) group and alcohol + verbenin (50mg / kg) / kg) group. Two mice per cage were used to establish a mouse model of alcoholic liver injury.

[0035] Mice in all groups were adaptively fed with co...

Embodiment 2

[0041] Effects of verbenaside on alcohol-induced liver pathology in mice

[0042] The liver tissue of each group of mice in Example 1 was fixed in a formalin solution for 24 h to 48 h, and the samples were dehydrated in alcohol and transparent in xylene, and then embedded in paraffin. The embedded tissue blocks were hardened and sliced ​​with a microtome. Before staining, the paraffin in the paraffin section was removed in xylene, dehydrated with ethanol from high concentration to low concentration, and finally rinsed with clean water (distilled water) to start staining. Stain in hematoxylin staining solution for 5min-15min, and wash off excess staining solution with running water. In 1% hydrochloric acid ethanol for 1 ~ 3s, after a little washing, add blue solution (0.5% light ammonia) to return to blue for 10 ~ 30s, then dye with 0.5% eosin for about 1 ~ 3min. After a little washing with distilled water, dehydration is carried out in a low-concentration to high-concentrati...

Embodiment 3

[0045] Effects of verbenaside on alcohol-induced serum ALT and AST in mice

[0046]Obtain the mouse blood serum obtained in Example 1 as a sample, and operate according to the instructions of the alanine aminotransferase (ALT) kit and aspartate aminotransferase (AST) kit (microwell 96T microplate method) (Nanjing Jiancheng Bioengineering Institute).

[0047] Detection of serum ALT and AST results such as Figure 3A , Figure 3B shown. Compared with alcohol-induced ALI mice, verbenaside (12.5, 25, 50 mg / kg) could significantly reduce serum ALT and AST levels (p < 0.01) (Figure 3), suggesting that verbenaside has protection against alcohol-induced ALI effect.

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Abstract

The invention relates to application of verbenin in a medicine for treating alcoholic liver injury. The verbenin can reduce AST and ALT levels of ALI mouse serum, plays a role in protecting acute liver injury caused by alcohol by inhibiting ferroptosis, and has the prospect of being developed into the medicine for preventing and treating alcoholic liver injury.

Description

technical field [0001] The invention belongs to the technical field of drug therapy, and particularly relates to the application of verbenaside in medicines for preventing and / or treating alcoholic liver damage. Background technique [0002] Alcoholic liver injury is a type of liver injury induced by long-term excessive drinking. According to its pathological characteristics, alcoholic liver injury can progress to alcoholic fatty liver, alcoholic hepatitis, alcoholic liver fibrosis, alcoholic liver cirrhosis and even liver cancer. Wait. With the growth of the economy, the number of alcohol abusers in the world is increasing, and the number of deaths caused by alcohol is also increasing. Alcoholic liver injury has become a problem that cannot be ignored worldwide, with its high morbidity and mortality causing people wide attention. [0003] Alcohol metabolism accumulates large amounts of free radicals and reactive oxygen species (ROS), leading to redox and antioxidant defen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P1/16
CPCA61K31/7048A61P1/16Y02A50/30
Inventor 马陶陶黄成张磊吕雄文冯蕊董佳慧臧洪梅
Owner ANHUI MEDICAL UNIV
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