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Homologous cancer cell membrane coated nucleic acid-chemotherapeutic drug complex nanoparticles

A chemotherapy drug and cancer cell membrane technology, applied in the field of medicine, can solve the problems of lack of targeted enrichment of tumor lesions and biological toxicity, and achieve the effects of reducing toxic side effects, improving targeting, and reversing tumor drug resistance

Active Publication Date: 2022-08-09
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

[0005] The second object of the present invention is to provide a preparation method based on homologous cancer cell membrane-coated nucleic acid-chemotherapy drug complex nanoparticles to solve the problem of exogenous carrier bands in the co-delivery of nucleic acid and chemotherapeutic drugs in the prior art. biological toxicity and lack of targeted enrichment of tumor lesions

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  • Homologous cancer cell membrane coated nucleic acid-chemotherapeutic drug complex nanoparticles
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  • Homologous cancer cell membrane coated nucleic acid-chemotherapeutic drug complex nanoparticles

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Embodiment 1

[0055] The nucleic acid-chemotherapeutic drug complex nanoparticle coated with homologous cancer cell membrane and a preparation method thereof include the following steps:

[0056] 1. Preparation of composite nanoparticle cores

[0057] 1.1 Preparation of mitoxantrone Mito and anti-apoptotic ASO (Bcl-2) complex nanoparticles (NP[Bcl-2&Mito])

[0058] Step 1, fully dissolve 100 μL of mitoxantrone Mito (250 g / mL) in 400 μL of water to prepare a Mito solution;

[0059] Step 2, fully dissolve 40 μL of anti-apoptotic ASO (Bcl-2) (100 μM) in 460 μL of water to prepare a Bcl-2 solution; I don’t think it is used in the above.

[0060] Wherein, the specific sequence of anti-apoptotic ASO (Bcl-2) is: 5'-TTT TCT CCC AGC GTG CGC CAT-3' (SEQ ID NO: 2)

[0061] In step 3, the Bcl-2 solution was slowly added dropwise to the Mito solution, vortexed for 2-3 minutes, and allowed to stand overnight to obtain a composite drug nanoparticle core (NP[Bcl-2&Mito]).

[0062] 1.2 Preparation of Pix...

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Abstract

The invention relates to the technical field of nano medicines, in particular to oligonucleotide drugs (antisense nucleotide: ASO; the invention relates to a preparation technology of nanoparticles co-assembled by small interfering nucleic acid (siRNA) and chemotherapeutic drugs, homologous cancer cell membrane coating and application thereof in the aspects of active targeting tumor combined treatment and tumor drug resistance reversal. The invention relates to a homologous cancer cell membrane coated oligonucleotide and chemotherapeutic drug co-delivery nano-drug system. The system is formed by taking oligonucleotide-chemotherapeutic drug compound nanoparticles as an inner core and taking a homologous cancer cell membrane as a shell. Compared with the prior art, the method has the advantages that the preparation process is simple, the electropositive chemotherapeutic drug is adopted to load ASO or siRNA, the biological safety problem caused by an exogenous carrier is avoided, and the toxic and side effects of the chemotherapeutic drug are reduced.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a nucleic acid-chemotherapeutic drug complex nanoparticle coated with homologous cancer cell membranes, in particular to a carrier-free nucleic acid-chemotherapy used for tumor targeted combined therapy and reversal of tumor drug resistance A preparation method of a drug complex nanoparticle and a method for coating the homologous cancer cell membrane of the nanoparticle. Background technique [0002] Malignant tumors are one of the most important diseases affecting human survival and health. Due to the complexity of tumor development, migration and drug resistance mechanisms, single chemotherapy (chemotherapy) or oligonucleotide therapy has many defects. Difficulty meeting treatment needs. In recent years, the combination therapy of chemotherapy and oligonucleotide therapy can reduce the dose and the toxic and side effects of drugs, and enhance the anti-tumor effect, which has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/46A61K45/06A61K31/7088A61P35/00
CPCA61K9/5176A61K9/5192A61K45/06A61K31/7088A61P35/00A61K2300/00Y02A50/30
Inventor 颜德岳朱利娟陈天保徐杰黄卫
Owner SHANGHAI JIAO TONG UNIV
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