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In-vitro culture method of autologous breast milk stem cells, injection and application of injection in skin injury repair

An in vitro culture and stem cell technology, applied to skin diseases, animal cells, etc. fixed on or in inorganic carriers, can solve problems such as unclear mechanism of action, and achieve the effect of promoting scar repair

Active Publication Date: 2022-08-02
郑州源创基因科技有限公司 +1
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  • Abstract
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  • Claims
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Problems solved by technology

[0003] Among them, the transplantation of stem cells has also provided great progress in the research of wound healing, but the specific mechanism of action is still unclear, and further exploration is still needed to explore and verify its mechanism of wound healing, as well as a large number of clinical application studies to conduct in-depth exploration research , to determine the ideal stem cell source and the most effective cell delivery method, promote wound healing, bring good news to trauma patients, and also provide help for its application in skin wrinkles, scar removal, anti-aging and other fields

Method used

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  • In-vitro culture method of autologous breast milk stem cells, injection and application of injection in skin injury repair
  • In-vitro culture method of autologous breast milk stem cells, injection and application of injection in skin injury repair
  • In-vitro culture method of autologous breast milk stem cells, injection and application of injection in skin injury repair

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Embodiment Construction

[0033] In order to make the purpose, technical solutions and advantages of the present application more clear, the present application will be further described in detail below with reference to the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application, but not to limit the present application.

[0034] In vitro culture of autologous breast milk stem cells (BSCs)

[0035] In the examples of the present application, autologous breast milk stem cells are used for in vitro culture to obtain an in vitro cultured injection. The injection contains a complex loaded with breast milk stem cells, and the complex has the effect of repairing scarred skin in rats. The specific preparation process includes the separation of BSCs, the process of cell mechanical tension culture treatment and the preparation of complexes. The specific process is as follows:

[0036] 1. Materials and methods

[0037] 1.1. Isolati...

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Abstract

The invention discloses an in-vitro culture method and injection of autologous breast milk stem cells and application of the autologous breast milk stem cells in skin injury repair. The in-vitro culture method comprises the following steps: separating breast milk stem cells; the method comprises the following steps: performing induced differentiation on breast milk stem cells to differentiate the breast milk stem cells into fibroblasts, and culturing the breast milk stem cells in a tension culture medium under the condition of mechanical tension in induced differentiation; loading and culturing fibroblasts to obtain a complex which is a complex of the polylactic acid hydrogel and the fibroblasts. The fibroblast efficiently expresses cell waveform protein and fibronectin and highly expresses I / III type collagen. The complex can promote efficient expression of scar skin I / III type collagen and normalization of I / III type collagen egg fiber arrangement through a PI3K / Akt signal channel mechanism, has the function of promoting scar repair, and has the application prospects of being widely applied to wound repair, skin wrinkle and scar removal, aging resistance and the like.

Description

technical field [0001] The present application relates to the technical field of skin damage repair, in particular to an in vitro culture method of autologous breast milk stem cells, an injection and its application in skin damage repair. Background technique [0002] Stem cells have great potential in skin injury repair and tissue regeneration. They can not only regenerate lost tissue, but also promote wound repair and healing through paracrine means. At present, embryonic stem cells, induced pluripotent stem cells, mesenchymal stem cells, tissue stem cells, epithelial stem cells, adipose stem cells and hematopoietic stem cells have all become research hotspots in skin injury repair and tissue regeneration. [0003] Among them, the transplantation of stem cells has also provided great progress for the research of wound healing, but the specific mechanism of action is still unclear, and further exploration is needed to explore and verify its mechanism of wound healing, as we...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775C12N5/077C12N11/096C12N11/14C12N13/00A61K35/33A61K9/06A61K47/34A61K47/42A61P17/02A61P17/00
CPCC12N5/0668C12N5/0656C12N11/096C12N11/14C12N13/00A61K35/33A61K9/06A61K47/34A61K47/42A61K9/0024A61P17/02A61P17/00C12N2506/1392C12N2501/30C12N2500/30C12N2501/113C12N2501/115C12N2501/119C12N2500/38C12N2500/36C12N2500/32
Inventor 王智亮温泉
Owner 郑州源创基因科技有限公司
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