Preparation method and application of bionic multifunctional composite sponge dressing
A composite sponge and multi-functional technology, applied in medical science, absorbent pads, bandages, etc., can solve the problems of low release efficiency of active ingredients, hindering the release of active ingredients, and decreased healing effect of dressings, etc., to promote collagen deposition, inhibit Bacterial infection, the effect of accelerating the wound healing process
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Embodiment 1
[0039] A preparation method of a bionic multifunctional composite sponge dressing, specifically comprising the steps of:
[0040] (1) Preparation of hyaluronic acid grafted with dopamine:
[0041] Weigh 2g of sodium hyaluronate with a molecular weight of 9-10kDa and dissolve it in 100mL of first-class water, stir magnetically at room temperature for 1 to 2 hours, and adjust the pH of the solution to 5; control the reaction temperature at 5°C, add 1.91g of EDC, 2.30 g of NHS, stirred for 2 h; vacuumed for 25 min, added 2 g of dopamine hydrochloride, stirred thoroughly for 30 min, vacuumed again for 25 min, and reacted at 6°C for 30 h; (pH=4) dialysis, then dialysis with deionized water until the absorbance of the dialysate at a wavelength of 280nm is zero, and the solution is lyophilized to obtain dopamine-grafted hyaluronic acid;
[0042] (2) Use tyrosinase to realize cross-linking:
[0043] Prepare 1% (w / v) dopamine-grafted hyaluronic acid solution and 3% (w / v) gelatin solu...
Embodiment 2
[0047] A preparation method of a bionic multifunctional composite sponge dressing, specifically comprising the steps of:
[0048] (1) Preparation of hyaluronic acid grafted with dopamine:
[0049] Weigh 2g of sodium hyaluronate with a molecular weight of 9-10kDa and dissolve it in 100mL of first-class water, stir magnetically at room temperature for 1-2 hours, and adjust the pH of the solution to 4.5; control the reaction temperature at 5°C, add 1.91g of EDC, 2.30 g of NHS, stirred for 1 h; vacuumed for 20 min, added 4 g of dopamine hydrochloride, stirred thoroughly for 25 min, vacuumed again for 20 min, and reacted at 6°C for 26 h; (pH=3) dialysis, then dialyzed with deionized water until the absorbance of the dialysate at a wavelength of 280nm is zero, and the solution is lyophilized to obtain dopamine-grafted hyaluronic acid;
[0050] (2) Use tyrosinase to realize cross-linking:
[0051] Prepare 1% (w / v) dopamine-grafted hyaluronic acid solution and 3% (w / v) gelatin solut...
Embodiment 3
[0055] A preparation method of a bionic multifunctional composite sponge dressing, specifically comprising the steps of:
[0056] (1) Preparation of hyaluronic acid grafted with dopamine:
[0057] Weigh 2g of sodium hyaluronate with a molecular weight of 9-10kDa and dissolve it in 100mL of first-class water, stir magnetically at room temperature for 1 to 2 hours, and adjust the pH of the solution to 5.5; control the reaction temperature at 4°C, add 1.91g of EDC, 2.30 g of NHS, stirred for 1 h; vacuumed for 15 min, added 3.80 g of dopamine hydrochloride, stirred thoroughly for 20 min, vacuumed again for 15 min, and reacted at 4°C for 24 h; HCl (pH=3.5) dialysis, then dialysis with deionized water until the absorbance of the dialysate at a wavelength of 280nm is zero, and the solution is lyophilized to obtain dopamine-grafted hyaluronic acid;
[0058] (2) Use tyrosinase to realize cross-linking:
[0059] Prepare 1% (w / v) dopamine-grafted hyaluronic acid solution and 3% (w / v) g...
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