Insect-killing and disease-preventing compound microbial agent and biological fertilizer containing microbial agent
A compound microbial agent, insecticide and disease prevention technology, applied in biocides, microbial-based methods, biocides, etc., can solve problems such as reduced fertility, soil nutrient imbalance, and increased drug resistance of underground pests, and achieve growth Improvement, environmental friendliness, broad application prospects and the effect of development space
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Embodiment 1
[0032] Embodiment 1 Determination of antibacterial spectrum of compound microorganism sterile filtrate
[0033] Select cotton blight, cotton fusarium wilt, cotton black rot, potato early blight, potato fusarium wilt, potato ring rot, potato dry rot, tobacco scab, tomato fusarium wilt, cotton red rot, pepper bacterial wilt Bacteria, corn leaf spot, corn sheath blight, wheat smut, peanut root rot, grape white rot, tomato early blight, cucumber botrytis, cucumber powdery mildew, cucumber fusarium wilt, cucumber anthracnose Target, the plate confrontation method was used to determine the inhibition of ZLP-101, D1, P-6; ZLP-101, BSD-2, D1; ZLP-101, CH2-22, BL-9, K5, three kinds of compound microorganism sterile filtrates. bacterial spectrum. It can be seen from the implementation data in Table 1 that the mixed sterile filtration of these functional microorganisms has a good inhibitory effect on different pathogenic bacteria, indicating that the antibacterial spectrum of this compo...
Embodiment 2
[0038] A kind of insecticide and disease prevention biological fertilizer (formula 1), the preparation method is as follows:
[0039] (1) Bacillus velesi ZLP-101 (the deposit number is CGMCC No. 20130), silicate bacteria K5, Bacillus amyloliquefaciens D1 and Bacillus subtilis BSD-2 (the deposit number is CGMCC No. 20129) , use four-district streaking method to inoculate and cultivate single colony on solid medium respectively; wherein the solid medium of Bacillus velesi ZLP-101, Bacillus amyloliquefaciens D1 and Bacillus subtilis BSD-2 is NB medium ( Beef extract 5g / L, peptone 10g / L, NaCl 5g / L, glucose 10g / L, agar 12.0g / L, pH 7.1±0.1), placed in a 37°C incubator for 18-24h. Solid medium of silicate bacteria K5 (yeast extract powder 5.0g / L, calcium carbonate 0.1g / L, calcium chloride 0.04g / L, K 2 HPO 4 ·3H 2 O 1.67g / L, KH 2 PO 4 0.87g / L, FeCl 3 ·6H 2 O 0.004g / L, MgSO 4 ·7H 2 O: 0.1g / L, agar 15.0g / L, pH6.9±0.1), placed in a 25°C incubator for 48-60h.
[0040]The single...
Embodiment 3
[0047] A kind of insecticide and disease prevention biological fertilizer (formula 2), the concrete preparation method is as follows:
[0048] (1) Bacillus velesi ZLP-101, Bacillus subtilis BSD-2, Bacillus phosphate solubilization P-6, Bacillus brevis CH2-22 and Bacillus licheniformis were used on solid medium by four-division streak method Inoculate and cultivate a single colony; wherein the solid medium of Bacillus velesi ZLP-101, Bacillus brevis CH2-22, Bacillus subtilis BSD-2 and Bacillus licheniformis is NB medium (beef extract 5g / L, peptone 10g / L, NaCl 5g / L, glucose 10g / L, agar 12.0g / L, pH 7.1±0.1), placed in a 37°C incubator for 18-24h. The solid medium of Bacillus phosphate solubilization P-6 is inorganic phosphorus medium (sucrose 2g / L, glucose 2g / L, NH 4 Cl 1.5g / L, KCl 0.3g / L, MgSO 4 .7H 2 O 0.4g / L, NaCl 0.2g / L, calcium phosphate 20g / L, pH 7.0), placed in a 32°C incubator for 20-24h.
[0049] Then inoculate the single colony in 100ml of liquid culture medium resp...
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