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Insect-killing and disease-preventing compound microbial agent and biological fertilizer containing microbial agent

A compound microbial agent, insecticide and disease prevention technology, applied in biocides, microbial-based methods, biocides, etc., can solve problems such as reduced fertility, soil nutrient imbalance, and increased drug resistance of underground pests, and achieve growth Improvement, environmental friendliness, broad application prospects and the effect of development space

Active Publication Date: 2022-08-02
BIOLOGY INST OF HEBEI ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the extension of the cultivation period and the long-term cumulative application of fertilizers, the resistance of underground pests is increasing, and the structure of microbial flora related to nutrient cycling, organic matter decomposition and disease resistance is unbalanced, resulting in soil-borne pathogens and underground pests. The increase in the number of pests, the imbalance of soil nutrients, and a series of problems such as aggravation of pests and diseases, reduction of fertility, and changes in physical and chemical properties will eventually lead to a decline in crop yields, seriously hindering the sustainable development of agriculture and food safety. There is an urgent need for environmentally friendly, economical and effective. Bio-organic fertilizers suitable for crops can reduce the incidence of crop diseases and insect pests on the basis of effectively ensuring high-quality product supply

Method used

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  • Insect-killing and disease-preventing compound microbial agent and biological fertilizer containing microbial agent
  • Insect-killing and disease-preventing compound microbial agent and biological fertilizer containing microbial agent
  • Insect-killing and disease-preventing compound microbial agent and biological fertilizer containing microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 Determination of antibacterial spectrum of compound microorganism sterile filtrate

[0033] Select cotton blight, cotton fusarium wilt, cotton black rot, potato early blight, potato fusarium wilt, potato ring rot, potato dry rot, tobacco scab, tomato fusarium wilt, cotton red rot, pepper bacterial wilt Bacteria, corn leaf spot, corn sheath blight, wheat smut, peanut root rot, grape white rot, tomato early blight, cucumber botrytis, cucumber powdery mildew, cucumber fusarium wilt, cucumber anthracnose Target, the plate confrontation method was used to determine the inhibition of ZLP-101, D1, P-6; ZLP-101, BSD-2, D1; ZLP-101, CH2-22, BL-9, K5, three kinds of compound microorganism sterile filtrates. bacterial spectrum. It can be seen from the implementation data in Table 1 that the mixed sterile filtration of these functional microorganisms has a good inhibitory effect on different pathogenic bacteria, indicating that the antibacterial spectrum of this compo...

Embodiment 2

[0038] A kind of insecticide and disease prevention biological fertilizer (formula 1), the preparation method is as follows:

[0039] (1) Bacillus velesi ZLP-101 (the deposit number is CGMCC No. 20130), silicate bacteria K5, Bacillus amyloliquefaciens D1 and Bacillus subtilis BSD-2 (the deposit number is CGMCC No. 20129) , use four-district streaking method to inoculate and cultivate single colony on solid medium respectively; wherein the solid medium of Bacillus velesi ZLP-101, Bacillus amyloliquefaciens D1 and Bacillus subtilis BSD-2 is NB medium ( Beef extract 5g / L, peptone 10g / L, NaCl 5g / L, glucose 10g / L, agar 12.0g / L, pH 7.1±0.1), placed in a 37°C incubator for 18-24h. Solid medium of silicate bacteria K5 (yeast extract powder 5.0g / L, calcium carbonate 0.1g / L, calcium chloride 0.04g / L, K 2 HPO 4 ·3H 2 O 1.67g / L, KH 2 PO 4 0.87g / L, FeCl 3 ·6H 2 O 0.004g / L, MgSO 4 ·7H 2 O: 0.1g / L, agar 15.0g / L, pH6.9±0.1), placed in a 25°C incubator for 48-60h.

[0040]The single...

Embodiment 3

[0047] A kind of insecticide and disease prevention biological fertilizer (formula 2), the concrete preparation method is as follows:

[0048] (1) Bacillus velesi ZLP-101, Bacillus subtilis BSD-2, Bacillus phosphate solubilization P-6, Bacillus brevis CH2-22 and Bacillus licheniformis were used on solid medium by four-division streak method Inoculate and cultivate a single colony; wherein the solid medium of Bacillus velesi ZLP-101, Bacillus brevis CH2-22, Bacillus subtilis BSD-2 and Bacillus licheniformis is NB medium (beef extract 5g / L, peptone 10g / L, NaCl 5g / L, glucose 10g / L, agar 12.0g / L, pH 7.1±0.1), placed in a 37°C incubator for 18-24h. The solid medium of Bacillus phosphate solubilization P-6 is inorganic phosphorus medium (sucrose 2g / L, glucose 2g / L, NH 4 Cl 1.5g / L, KCl 0.3g / L, MgSO 4 .7H 2 O 0.4g / L, NaCl 0.2g / L, calcium phosphate 20g / L, pH 7.0), placed in a 32°C incubator for 20-24h.

[0049] Then inoculate the single colony in 100ml of liquid culture medium resp...

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Abstract

The invention relates to an insect-killing and disease-preventing compound microbial agent and a biological fertilizer containing the same. The insect-killing and disease-preventing compound microbial agent and an organic fertilizer are mixed according to the mass ratio of 1: (1000-1100) to obtain the insect-killing and disease-preventing compound microbial agent. The insect-killing and disease-preventing compound microbial agent is obtained by mixing and adsorbing fermentation liquor of microorganisms and turf according to the mass ratio of 1: 3. The fermentation liquor of the microorganisms comprises bacillus velezensis ZLP-101 fermentation liquor and additional bacteria fermentation liquor in a mass ratio of (50-60): (46-42); the additional bacteria comprise more than three of silicate bacteria K5, bacillus amyloliquefaciens D1, bacillus subtillis BSD-2, bacillus phosphate solubilizing P-6, brevibacillus brevis CH2-22 and bacillus licheniformis BL-9. The composition is harmless to human and livestock, is environment-friendly, and can be widely applied to prevention and control of crop diseases and insect pests and yield increase.

Description

technical field [0001] The invention relates to the technical field of fertilizers, in particular to an insecticidal and disease-preventing compound microbial inoculant and a biological fertilizer containing the inoculum. Background technique [0002] To a certain extent, chemical fertilizers and chemical pesticides provide nutrients for crops, reduce the incidence of underground pests, and indirectly increase crop yields. However, with the extension of cultivation years and the long-term cumulative application of fertilizers, the resistance of underground pests is increasing, and the microbial flora structure related to nutrient cycling, organic matter decomposition and disease resistance is unbalanced, resulting in soil-borne pathogens and underground pests. The number of pests increases, soil nutrients are unbalanced, a series of problems such as aggravation of pests and diseases, reduction of fertility, and changes in physical and chemical properties occur, which ultimat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01N63/20A01P3/00A01P7/04C05G3/60C12R1/01C12R1/07C12R1/10C12R1/125
CPCC12N1/20A01N63/22A01N63/20C05G3/60C05F3/00C05F5/00C05F5/002C05F11/00C05F11/02Y02W30/40
Inventor 王雅娜张飞燕张丽萍刘洪伟李雪琨赵雯雅董尧坤李荣王瑞文
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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