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New application of volatile compound of liquorice and alhagi sparsifolia

A volatile compound, camel thorn technology, applied in the application, pest control, attracting pests and other directions, can solve the problems of weak pest control and poor adaptability

Pending Publication Date: 2022-08-02
INST OF PLANT PROTECTION OF XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the unique ecological environment and climatic conditions in the existing Xinjiang area, outstanding problems such as poor adaptability and weak damage control often occur after natural enemy resources in other areas are introduced into Xinjiang, the technical problem to be solved by the present invention: provide a kind of licorice The new use of anti-toxic compounds, through this method, the luring rate of the important predatory ladybug, an important predator of various pests such as cotton black aphid in Xinjiang, has been improved, and the damage of various pests such as aphids to cotton and other crops has been reduced.

Method used

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  • New application of volatile compound of liquorice and alhagi sparsifolia
  • New application of volatile compound of liquorice and alhagi sparsifolia
  • New application of volatile compound of liquorice and alhagi sparsifolia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Analysis and identification of active volatiles in licorice and camel thorn attracting L. polyxii

[0030] The present embodiment provides a method for analyzing and identifying active volatiles in licorice and camel thorn volatiles, which specifically includes the following steps:

[0031] (1) Analysis of active volatiles

[0032]The GC-EAD reaction was carried out by gas chromatography GC and EAG instrument. Use the prepared reference electrode to align the base of the head of P. versicolor and gently insert it and mount it on the micromanipulator. On the other side, the pre-installed reference electrode is aligned with the antenna by adjusting the knob of the operating table, and the tip of the antenna is inserted into the capillary of the recording electrode with the aid of an insect needle. The two electrodes are connected by a silver wire to form a closed circuit. The EAG system Connect; wait for 1-3 min, and after the antenna potential signal is stab...

Embodiment 2

[0036] Example 2: Antennae Potential Responses to Active Volatile Compounds

[0037] The antenna connection method and the EAG connection method are the same as above. After the EAG signal was stabilized, the antennae were stimulated with active volatiles, and the response values ​​of the antennal potential were recorded. The order of taste source stimulation was: control, reference, 0.01 mg / mL, 0.1 mg / mL, 1 mg / mL, 10 mg / mL, 100 mg / mL, reference and control. Insert the tip of the Pasteur dropper into the steel tube (about 3mm in depth) through the small hole on the tube wall (about 11cm from the mouth of the tube), the stimulation time is 0.5s, and the interval between two stimulations is 30s. The female and male adult ladybugs were repeated 8 times each. The EAG instrument is connected to the computer through the IDAC-2 signal collector. The antenna is about 1mm away from the mouth of the steel pipe, and an air flow of 400 mL / min enters the steel pipe (length 14 cm, inner ...

Embodiment 3

[0039] Example 3: Behavioral Behavioral Responses of Coccinella polyxietys to Active Volatiles

[0040] A Y-type olfactometer was used to test the chemotactic selection behavior of Coccinellidae to two concentrations of active volatiles. The Y-type olfactometer was placed in a dark room of 100×100×60cm, and two 40W fluorescent lamps (light intensity 700lux) were hung 40cm above the olfactometer. The base is connected to the release tube of the adult ladybug, and the two arms are connected to different odor sources with Teflon tubes. One of the small glass tubes connected with one arm contains filter paper strips (5×0.5 cm) dipped in paraffin oil; the small glass tubes connected by the other arm contains filter paper strips dipped in different active volatiles. An atmospheric sampler was used as the airflow power system, the flow rate was set to 0.4L / min, and the airflow was filtered by activated carbon and humidified with distilled water into the flavor source.

[0041] The ...

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Abstract

The invention discloses a novel application of a volatile compound of liquorice and alhagi sparsifolia, and the volatile compound of the liquorice and the alhagi sparsifolia is used for luring adults of polyladybird, which is an important predatory natural enemy in Xinjiang regions, so as to control the harm of various aphids such as gossypius gossypii aphis and the like. The liquorice and alhagi sparsifolia volatile compound is one or a combination of more of alpha-pinene, L-beta-pinene, butyl acrylate, butyl isobutyrate, butyl butyrate, 1, 3-diethylbenzene and 1, 4-diethylbenzene. A liquorice and alhagi sparsifolia-cotton black aphid-polyheterodyne three-level nutrition chain is used as a research system, liquorice and alhagi sparsifolia volatile matters which are damaged by cotton black aphid feeding are screened and separated, the result shows that the reaction rates of polyheterodyne adults to the seven volatile matters indoors are relatively high, and the luring rate of one or more combinations to the polyheterodyne adults is 50-85%; the field trapping effect on the adult ladybird is remarkable, various pests such as aphids can be effectively controlled, and good application and popularization value is achieved.

Description

technical field [0001] The invention belongs to the technical field of plant protection attractants, in particular to the technical field of new uses of licorice and camel thorn volatile compounds. Background technique [0002] Aphids are one of the main cotton pests in Xinjiang and are the key factor limiting cotton yield. At present, insects such as cotton aphids mainly rely on chemical control, but the aphids are small in size, high in fertility and strong in adaptability, and the extensive application of chemical pesticides has led to their resistance. There is an urgent need for a pest control strategy that can replace chemical pesticides with less harm to the ecosystem. The ecological cost of plant volatiles is small, and by changing the non-toxic mode of action of pests and natural enemies, it is difficult to make insects resistant, and they can be developed and utilized as an important tool in the field of pest management. [0003] As we all know, in the long-term ...

Claims

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Application Information

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IPC IPC(8): A01N65/20A01N27/00A01N37/06A01N37/02A01P19/00
CPCA01N65/00A01N65/20A01N27/00A01N37/06A01N37/02A01P19/00Y02A50/30
Inventor 潘洪生姜岩李号宾王冬梅丁瑞丰
Owner INST OF PLANT PROTECTION OF XINJIANG ACADEMY OF AGRI SCI
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