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Preparation method and application of multichannel microfluidic electrochemical sensing chip

A sensor chip and multi-channel technology, which is applied in the field of nanomaterials and electrochemical sensing, can solve the problems of reducing sensor selectivity and sensitivity, and achieve enhanced electron transfer and material transport capabilities, low density, and adjustable pores. Effect

Active Publication Date: 2022-07-29
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, a major challenge of liquid biopsy is that the analyte is often in a complex body fluid environment, such as serum, plasma, etc., and the impurity proteins and other biomacromolecules in it may be non-specifically adsorbed on the surface of the sensor, reducing the selection of the sensor. Therefore, it is necessary to consider the construction of high-sensitivity, anti-pollution sensing interface

Method used

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  • Preparation method and application of multichannel microfluidic electrochemical sensing chip
  • Preparation method and application of multichannel microfluidic electrochemical sensing chip
  • Preparation method and application of multichannel microfluidic electrochemical sensing chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Preparation of Zn-TCPP / GO multi-channel microfluidic electrochemical sensor chip:

[0046] A. Evenly disperse 5mg of graphene oxide in 13mL of absolute ethanol, after ultrasonic treatment for 10min, obtain a uniform graphene oxide dispersion, and evenly coat the dispersion on the surface of the silicon wafer, and wait for it to dry to form graphite oxide vinyl film layer;

[0047] B, the anhydrous ethanolic solution of the anhydrous zinc acetate with a concentration of 4mM is coated on the graphene oxide film layer combined on the silicon wafer obtained in step A, and dried to obtain the graphene oxide film layer and the zinc acetate film layer combined successively. Silicon wafer; then calcined at 350°C for 30 minutes, take out the silicon wafer and cool it naturally to obtain a silicon wafer combined with zinc oxide / graphene oxide composite material;

[0048] C. In a system with anhydrous ethanol:N,N-dimethylformamide (DMF) volume ratio of 3:1, it is formulated to co...

Embodiment 2

[0055] Preparation of Cu-TCPP / GO multi-channel microfluidic electrochemical sensor chip:

[0056] A. 5mg graphene oxide is uniformly dispersed in 13mL absolute ethanol, after ultrasonic treatment for 10min, a uniform graphene oxide dispersion solution is obtained, the dispersion solution is evenly coated on the surface of the silicon wafer, and it is dried to form graphite oxide vinyl film layer;

[0057] B. the dehydrated ethanol solution of the anhydrous zinc acetate with a concentration of 4mM is coated on the graphene oxide film layer combined on the silicon wafer obtained in step A, and dried to obtain the graphene oxide film layer and the zinc acetate film layer in combination successively. Silicon wafer; then calcined at 350°C for 30 minutes, take out the silicon wafer and cool it naturally to obtain a silicon wafer combined with zinc oxide / graphene oxide composite material;

[0058] C. In a system with anhydrous ethanol:N,N-dimethylformamide (DMF) volume ratio of 3:1,...

Embodiment 3

[0065] The multi-channel microfluidic electrochemical sensor chip prepared in Example 1 detects goat anti-human IgG:

[0066] A. The ProteinA / PBS solution (pH=7.4) was injected into the electrochemical sensor chip channel through the injection tank I 10, and then the electrochemical sensor chip was incubated at 4°C overnight. ProteinA and the multi-channel electrode chip surface metal Ion complexation plays a role as a bridge for subsequent immobilization of antibodies;

[0067] B, the phosphate buffered saline solution is slowly passed into the electrochemical sensor chip channel through the injection pool II 11 to clean the entire channel;

[0068] C. Inject the goat anti-human IgG antibody / PBS solution into the channel of the electrochemical sensor chip through the injection pool I 10, requiring the previous phosphate buffered saline solution in the channel to be discharged from the channel, and then place the electrochemical sensor chip at 37 ° C. Incubate for 1 h, and bi...

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Abstract

The invention discloses a preparation method and application of a multi-channel micro-fluidic electrochemical sensing chip, and belongs to the technical field of nano materials and electrochemical sensing. The multi-channel microfluidic electrochemical sensing chip is prepared by the following steps: firstly preparing a sensitive composite material, then preparing printing ink from the material, coating the printing ink on the surface of a working electrode of the sensor chip, and further compounding and packaging with a microfluidic channel. According to the invention, the micro-fluidic channel is integrated on the surface of the multi-channel electrode chip, so that a trace sample is accurately controlled, various multi-channel and serialized operations and experiments can be conveniently carried out, and the multi-channel electrode chip is more suitable for detection and research on a trace substance to be detected in a complex environment. The micro electrochemical sensor suitable for parallel and joint detection of a plurality of objects to be detected can be developed by combining the electrochemical sensing chip array printed in batches with a micro-fluidic technology.

Description

technical field [0001] The invention relates to the technical field of nanomaterials and electrochemical sensing, in particular to a preparation method and application of a multi-channel microfluidic electrochemical sensing chip. Background technique [0002] Electrochemical biosensors detect biological entities by converting biological information into electrical signals. They are the most widely used in liquid biopsy and have the advantages of convenient sample acquisition, low invasiveness (blood, urine, saliva, etc.) Simultaneous detection of multiple samples and many other advantages. However, a major challenge faced by liquid biopsy is that the test object is often in a complex body fluid environment, such as serum, plasma, etc., and biological macromolecules such as impurity proteins may be non-specifically adsorbed on the sensor surface, reducing the selection of sensors. Therefore, it is necessary to consider the construction of a high-sensitivity, pollution-resist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416B01L3/00
CPCG01N27/416B01L3/5027
Inventor 张源诸昊应孜邓名仕薛厂高兴华
Owner SHANGHAI UNIV
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