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Detection method of thrombus-related gene chip

A gene chip and detection method technology, applied in the field of biological detection, can solve the problems of low accuracy, high detection difficulty and high cost, and achieve the effects of high accuracy, good coverage and low cost

Pending Publication Date: 2022-07-29
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Description
  • Claims
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Problems solved by technology

[0006] The purpose of the present invention is to provide a detection method of a thrombus-related gene chip, which aims to solve the problems of low accuracy, high cost and relatively difficult detection of the traditional detection method of a thrombus-related gene chip

Method used

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  • Detection method of thrombus-related gene chip
  • Detection method of thrombus-related gene chip
  • Detection method of thrombus-related gene chip

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Embodiment 1

[0041] like figure 1 , figure 2 , image 3 As shown, it is a detection method of a thrombus-related gene chip provided by the present invention, and its specific embodiment is as follows:

[0042] The selection of specific and effective gene sets is very important for the screening of thrombosis-related genes. Missing selection will reduce the detection sensitivity, and multiple selection will increase the cost and difficulty of operation;

[0043] Based on the above situation, the present invention conducts extensive screening of related gene mutations involved in systemic pathways such as coagulation, platelets, and fibrinolysis in the process of thrombosis by inquiring a large number of research documents on gene mutations related to thrombosis; The research progress of related gene mutation sites in ClinVar, HGMD, OMIM and other databases, summarize the research experience of all reported gene mutations that are clearly associated with thrombosis in terms of protein fun...

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Abstract

The invention provides a detection method of a thrombus-related gene chip, relates to the field of biological detection, and particularly discloses corresponding 62 pathogenic mutation sites in the thrombus-related gene chip, the detection method of the thrombus-related gene chip comprises probes for the following 21 thrombus-related genes: PROC, PROS1, SERPINC1, SERPINA10, THBD, PLG, GCKR, ADAMTS13, F2, MTHFR, SERPIND1, JAK2, PROCR, SERPINE1, F5, PROZ, STAB2, HRG, SLC44A2, SLC4A1 and VWF. According to the detection method of the thrombus-related gene chip, the probes are used for capturing the 21 thrombus-related genes; 62 pathogenic mutation sites of the gene can be specifically captured; a probe capture method next-generation sequencing technology is adopted, mutation of all exons and regulatory regions of the genes can be comprehensively and systematically detected at a time, and the invention aims to be applied to molecular diagnosis and biological research of thrombus caused by gene mutation. The method has the advantages of clear detection target, good coverage, high accuracy, stable detection, short period, low cost and the like.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a detection method of a thrombus-related gene chip. Background technique [0002] Thrombosis is a disease that can affect various organ systems of the whole body, involves multiple disciplines of clinical medicine, and seriously endangers human health. It has the characteristics of high morbidity and high mortality and disability. Most of the thrombotic diseases are prone to thrombosis, which refers to the disease caused by hereditary factors (coagulation, anticoagulation, hereditary defects of fibrinolysis system) or acquired factors (old age, pregnancy and puerperium, malignant tumor, etc.). Pathological states that are prone to thrombosis, among which hereditary thrombophilia is often unable to be diagnosed in time due to the etiology, which often affects the correct prevention and treatment, resulting in serious thrombosis events. In terms of the common types of hereditar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6874C12Q1/6883
CPCC12Q1/6874C12Q1/6883C12Q2600/156C12Q2531/113C12Q2525/191C12Q2525/173C12Q2565/501
Inventor 胡豫邓君唐亮梅恒丁雅洁
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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