Microorganism separation and inspection method

Abstract

The invention belongs to the technical field of microbiological detection methods, and particularly relates to a microbiological separation and detection method capable of improving the flow range control of a subsequently dropwise added culture solution. The method comprises the following steps: 1, diluting a microbial suspension, and mixing a small amount of diluent with an agar culture medium; 2, uniformly shaking the mixture to prepare a bacteria-containing agar plate; step 3, dropwise adding a culture solution to the local part of the bacterium-containing agar plate at intervals through liquid dropping culture equipment to form dispersed single bacterial colonies, shaking the base station fixing plate in a small range through a shaking assembly, and diffusing the range of the culture solution; 4, selecting bacterial colonies, and performing purification culture; the liquid dropping culture equipment comprises a culture box, cover plates, a frame opening, a light-transmitting plate, sealing frames, rubber strips, a material conveying pipe and a base station fixing plate, the cover plates are installed on the two sides of the culture box, the frame opening is formed in the top of the culture box, the light-transmitting plate is installed on the frame opening, and the sealing frames are symmetrically connected to the two sides of an opening in the upper side of the light-transmitting plate in a sliding mode.

Description

technical field [0001] The invention belongs to the technical field of microbial testing methods, in particular to a microbial separation testing method. Background technique [0002] The process of obtaining only one or a certain strain of microorganisms from a mixed microbial population is called microbial isolation and purification; in the research and application of molecular biology, it is not only necessary to separate specific microorganisms from the mixed microbial population by separation and purification technology. It is also necessary to pay attention to maintaining the purification of a single colony of microorganisms, which is convenient for subsequent microbial detection; the dilution and pouring plate method, the coating plate method and the dilution shaking tube method are often used for the separation of microbial strains, but for the subsequent dropwise addition of the culture solution The flow range is poorly controlled. SUMMARY OF THE INVENTION [000...

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Problems solved by technology

[0002] The process of obtaining only one or a certain strain of microorganisms from a mixed microbial population is called microbial isolation and purification; in the research and application of molecular biology, it is not only necessary to separate specific microorganisms from mixed microbial In addition, it is necessary to pay attention to the purification of a single microbial colony to facilitate subsequent microbial detection; the dilution pour plate method, the spread plate method and the dilution shake tube method are often used for the separation of microbial strains, but for the subsequent dropwise addition of the culture solution Poor control of flow range

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