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Method for producing collagen tripeptide from cow leather

A collagen tripeptide, cowhide technology, applied in the direction of peptides, specific peptides, connective tissue peptides, etc., can solve the problems of low purity, restricting the in-depth development and application of collagen tripeptide, and low yield of collagen tripeptide, and achieve water solubility. Good, shortening the enzymatic hydrolysis time, and the effect of low ash content

Active Publication Date: 2022-07-29
山东恒鑫生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing collagen tripeptides have low yield and low purity, which seriously restrict the in-depth development and application of collagen tripeptides

Method used

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  • Method for producing collagen tripeptide from cow leather

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for producing collagen tripeptide with cowhide is prepared by the following steps:

[0030] 1) Pretreatment: Take fresh cowhide, clean it, depilate it, cut it into small pieces no larger than 4×4mm, add it to a 5% sodium chloride solution, soak it at room temperature for 1 hour, control the moisture, and add to In 0.1mol / L NaOH solution, stir evenly at 4°C, soak for 3 days, and wash with water for 4 times to obtain pretreated cowhide;

[0031] Said, the weight ratio of cowhide to sodium chloride solution and NaOH solution is 1:5:4.

[0032] 2) Denaturation treatment: Mix the pretreated cowhide and water in a mass ratio of 1:5, boil at 100°C for 25min under 0.9MPa, cool down to 55°C, filter out the residue with double-layer gauze, and obtain denatured cowhide;

[0033] 3) Excitation treatment: add the modified cowhide to the excitation solution, soak it at 40°C for 40min, heat it up to 83°C, keep it for 4min, naturally cool to room temperature, wash with water,...

Embodiment 2

[0043] A method for producing collagen tripeptide with cowhide is prepared by the following steps:

[0044] 1) Pretreatment: Take fresh cowhide, clean it, remove hair, cut it into small pieces no larger than 4×4mm, add it to a sodium chloride solution with a mass fraction of 3%, soak it at room temperature for 1 hour, control the moisture, and add to In 0.1mol / L NaOH solution, stir evenly at 5°C, soak for 2 days, and wash with water for 5 times to obtain pretreated cowhide;

[0045] Said, the weight ratio of cowhide to sodium chloride solution and NaOH solution is 1:4:3.

[0046] 2) Denaturation treatment: mix the pretreated cowhide and water in a mass ratio of 1:5, boil at 100°C for 30min under the condition of 1.0MPa, cool down to 50°C, filter out the residue with double-layer gauze, and obtain the denatured cowhide;

[0047] 3) Excitation treatment: add the modified cowhide to the excitation solution, soak it at 35°C for 50 minutes, raise the temperature to 80°C, keep it f...

Embodiment 3

[0057] A method for producing collagen tripeptide with cowhide is prepared by the following steps:

[0058] 1) Pretreatment: Take fresh cowhide, clean it, depilate it, cut it into small pieces no larger than 4×4mm, add it to a sodium chloride solution with a mass fraction of 7%, soak it at room temperature for 1 hour, control the moisture, and add to In 0.1mol / L NaOH solution, stir evenly at 3°C, soak for 4 days, and wash with water for 3 times to obtain pretreated cowhide;

[0059] Said, the weight ratio of cowhide to sodium chloride solution and NaOH solution is 1:6:5.

[0060] 2) Denaturation treatment: mix the pretreated cowhide and water in a mass ratio of 1:5, boil at 100°C for 20min under 0.8MPa, cool down to 60°C, filter out the residue with double-layer gauze, and obtain denatured cowhide;

[0061] 3) Excitation treatment: add the modified cowhide to the excitation solution, soak for 30 minutes at 45°C, heat up to 85°C, hold for 3 minutes, naturally cool to room temp...

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Abstract

The invention provides a method for producing collagen tripeptide from cow leather, which comprises the following steps: pretreatment, denaturation treatment, excitation treatment, preparation of collagen tripeptide liquid, enzyme deactivation of the collagen tripeptide liquid, centrifugation, collection of supernate, decoloration and deodorization with a K15 activated carbon column, and spray drying to obtain the collagen tripeptide. According to the method disclosed by the invention, collagen can be thoroughly subjected to enzymolysis, the molecular weight of an enzymolysis product is small and concentrated, the collagen tripeptide yield is high, the hydroxyproline content is high, the total nitrogen content is high, the ash content is low, a finished product is fine and smooth, the water solubility is good, and large-scale production is facilitated.

Description

technical field [0001] The invention belongs to the technical field of active substance extraction, and particularly relates to a method for producing collagen tripeptide from bovine hide. Background technique [0002] Collagen is rich in glycine, proline, hydroxyproline and other collagen characteristic amino acids that the human body needs. Collagen is a high molecular functional protein. It is the main component of the skin, accounting for 80% of the dermis of the skin. It forms a fine elastic network in the skin, which firmly locks in moisture and supports the skin. Collagen is a spiral fibrous protein twisted by three peptide chains, and it is also the most abundant protein in the human body. It accounts for more than 30% of the total protein in the human body and is an important part of human cells, especially the extracellular matrix of the skin. Collagen is a macromolecular protein with a molecular weight above 300,000 Da and cannot be directly absorbed by the huma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K5/083C12P21/06
CPCC07K14/78C07K5/0806C12P21/06Y02P20/10
Inventor 张格林冰莹吴伟崔根李江
Owner 山东恒鑫生物科技有限公司
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