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Application of farnesene in preparation of plant-derived urease inhibitor

A technology of farnesene and source urease, which is applied in application, fertilizer mixture, agriculture, etc., can solve the problems of small impact on the ecological environment, unstable effect, environmental pollution, etc., achieve small impact on the ecological environment, solve high toxicity, and have good application prospects Considerable effect

Active Publication Date: 2022-07-29
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The urease inhibitors that have been identified mainly include heavy metal ions, metal complexes, phosphoramides, hydroxamic acids, etc. However, most of the above urease inhibitors have problems such as unstable effects, high toxicity, and easy degradation, so it is difficult to It is widely used in different regions, and long-term application may cause pollution to the environment. Compared with artificial chemically synthesized urease inhibitors, extracts from plants are natural compounds and have little impact on the ecological environment. Therefore, screening New high-efficiency and low-toxicity urease inhibitor can contribute to the green development of agriculture

Method used

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  • Application of farnesene in preparation of plant-derived urease inhibitor
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  • Application of farnesene in preparation of plant-derived urease inhibitor

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Embodiment 1

[0033] Application of acyclic sesquiterpenes in the preparation of plant-derived urease inhibitors:

[0034] (1) Preparation of the test solution

[0035] Weigh acetohydroxamic acid, prepare 0.1 μM, 0.5 μM, 1 μM, 3 μM, 5 μM, 10 μM, 20 μM, 40 μM, 60 μM, 80 μM of acetylhydroxamic acid solution with 20 mM HEPES buffer (pH 7.5), and place in Store in a brown bottle in a refrigerator at 4°C for future use;

[0036] Weigh the farnesene prepared in Example 1 of the present invention, and prepare 0.1 μM, 0.5 μM, 1 μM, 3 μM, 5 μM, 10 μM, 20 μM, 40 μM, 60 μM, 80 μM of acacia with 20 mM HEPES buffer (pH is 7.5). alkene solution, placed in a brown bottle and stored in a refrigerator at 4°C for future use;

[0037] (2) Preparation of Concanavali urease solution

[0038] Concanavalina urease was weighed, dissolved in 20 mM HEPES buffer (pH 7.5), prepared into a 10 U / mL concanavalina urease solution, and placed in a 4°C refrigerator for storage for later use.

[0039] (3) Preparation of ...

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Abstract

The invention discloses application of farnesene in preparation of a plant-derived urease inhibitor, and belongs to the technical field of urease inhibitors. The compound can effectively inhibit the activity of concanavalin urease and soil urease, and the effect of the compound can be equivalent to that of a commercial urease inhibitor acetohydroxamic acid. The urease inhibiting substance provided by the invention is derived from plants, has the characteristic of'green 'of a natural product, is high in biological safety, simple and stable in chemical structure and remarkable in urease inhibiting effect, can solve the problems of high toxicity, easiness in degradation and poor stability of a conventional chemically synthesized urease inhibitor, and has a wide application prospect. Meanwhile, a new thought is provided for creating a biological and ecological technology for efficiently inhibiting rapid hydrolysis of farmland urea and improving the utilization rate of urea nitrogen fertilizer, and good application value and development prospects are achieved.

Description

technical field [0001] The present invention relates to the technical field of urease inhibitors, in particular to the application of farnesene in the preparation of plant-derived urease inhibitors. Background technique [0002] Urea is the most common type of nitrogen fertilizer, with high nutrients and low cost, and is widely used in agricultural production. However, the direct application of urea has the disadvantages of fast nutrient conversion and low nitrogen utilization rate. The urea applied to the soil quickly generates ammonium nitrogen under the hydrolysis of urease, and only 30% to 60% of the nitrogen is absorbed and utilized by crops. A large amount of urea Application not only causes economic and resource waste, but also leads to environmental problems such as soil acidification and compaction, water eutrophication and air pollution. Since the 1960s, all countries in the world have carried out a series of research work on delaying urea hydrolysis and nutrient ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/40C05G3/90
CPCC05G3/40C05G3/90Y02P60/21
Inventor 宫平武志杰张丽莉李东坡
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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