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Photothermal sensor based on wettability paper chip and application of photothermal sensing method of photothermal sensor in biological detection

A sensing method and paper chip technology, applied in the field of biosensing, can solve the problems of uneven heat transfer, incapable of high-throughput, complex marking, etc., achieve the goal of reducing sample volume, shortening laser irradiation time, and improving detection sensitivity Effect

Pending Publication Date: 2022-07-26
HUBEI UNIV
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Problems solved by technology

[0004] Aiming at the problems that the existing photothermal sensing technology requires a large number of samples, complex marks, uneven heat transfer and high throughput, the present invention proposes a photothermal sensor and method based on wettable paper chips, and uses Taking miRNA as an example, in biological detection, the temperature signal is used as the output signal, and the temperature signal is read by a portable infrared temperature imager, which can realize the portable quantitative detection of the target

Method used

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  • Photothermal sensor based on wettability paper chip and application of photothermal sensing method of photothermal sensor in biological detection
  • Photothermal sensor based on wettability paper chip and application of photothermal sensing method of photothermal sensor in biological detection
  • Photothermal sensor based on wettability paper chip and application of photothermal sensing method of photothermal sensor in biological detection

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Embodiment

[0046] 1. Preparation of hydrophobic paper base: Cut cellulose filter paper into a size of 2.5cm×2.5cm, soak it in a n-hexane solution containing 3% octadecyltrichlorosilane, and react for 2h. The paper base was then rinsed with ethanol, and dried in an oven at 45°C to obtain a hydrophobic paper base ( figure 2 ), the contact angle of the hydrophobic surface is 146.6±2.3°.

[0047] 2. Preparation of super-hydrophilic microwell array: a steel mask plate with a thickness of 2.5cm×2.5cm and a thickness of 1mm is customized. There are 4×4 circular holes with the same size and a diameter of 1mm on the template. Cover the mask on the hydrophobic paper base and fix it with a long tail clip, place the paper base covered with the mask in a plasma cleaner for 5 s. The exposed part of the mask circular hole is re-converted to superhydrophilic ( image 3 ), while the covered part remains hydrophobic, resulting in a wettable paper base. like Figure 4 As shown in the figure, the red d...

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Abstract

The invention provides a photo-thermal sensing method based on a wettability paper chip, verifies biological detection application of the method by taking miRNA as an example, and belongs to the technical field of biosensors. A super-hydrophilic micro-well is constructed on a hydrophobic paper base, then a capture probe is modified in the super-hydrophilic micro-well, and the miRNA photo-thermal sensor is prepared. According to the photo-thermal sensor for miRNA detection, when miRNA exists, the capture probe, the miRNA and the reporter probe are subjected to hybridization of a sandwich structure, the reporter probe further triggers the H1 probe and the H2 probe to be subjected to hybridization chain reaction, C-rich sequences at the tails of the H1 probe and the H2 probe can adsorb a large amount of Ag < + >, and Ag2S nanoparticles are generated under the action of Na2S; the concentration of a target object is converted into temperature to be output by utilizing the photo-thermal performance of the Ag2S nanoparticles, and the portable infrared thermal imager is used for quantification, so that the immediate quantitative detection of miRNA can be realized. The photo-thermal sensing method based on the wettability paper chip has the advantages of small sample size, high sensitivity, low cost, high flux and the like.

Description

technical field [0001] The invention belongs to the technical field of biological sensing, and in particular relates to a photothermal sensor based on a wettable paper chip, a photothermal sensing method thereof, and an application in biological detection. Background technique [0002] Instant detection is a low-cost, simple, fast, portable detection technology that does not require professional technicians to operate. It not only meets the special requirements of time and space for detection, but also enables instant reporting of test results. However, in practical applications, due to the small amount of samples, the low concentration of markers, and many interference factors, the sensitivity and accuracy of the currently developed point-of-care detection methods need to be further improved, and most point-of-care detection methods use color changes to determine the target. Only qualitative or semi-quantitative detection can be achieved, so there is an urgent need to devel...

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Application Information

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IPC IPC(8): G01N21/17G01J5/48C12Q1/682
CPCG01N21/171G01J5/0003C12Q1/682G01J2005/0077
Inventor 刘志洪吴婷婷曹宇陈汉君
Owner HUBEI UNIV
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