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High-temperature-resistant pichia guilliermondii strain HGC34 and application of high-temperature-resistant pichia guilliermondii strain HGC34 in livestock and poultry manure deodorization and degradation

A technology of livestock and poultry manure and yeast, applied in the field of microorganisms, can solve problems such as ecological environment hazards, achieve the effect of protecting the environment and promoting rapid degradation and maturity

Pending Publication Date: 2022-07-22
HUANGGANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main components of the odor during the composting process include ammonia, hydrogen sulfide and volatile organic compounds (VOCs), which cause serious harm to the ecological environment

Method used

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  • High-temperature-resistant pichia guilliermondii strain HGC34 and application of high-temperature-resistant pichia guilliermondii strain HGC34 in livestock and poultry manure deodorization and degradation
  • High-temperature-resistant pichia guilliermondii strain HGC34 and application of high-temperature-resistant pichia guilliermondii strain HGC34 in livestock and poultry manure deodorization and degradation
  • High-temperature-resistant pichia guilliermondii strain HGC34 and application of high-temperature-resistant pichia guilliermondii strain HGC34 in livestock and poultry manure deodorization and degradation

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0035] Collect samples of extremely rotten chestnuts ( figure 1 The bacterial species in ) is separated and identified, and the isolated bacterial strain is extracted genomic DNA according to conventional methods, and PCR amplification is carried out after the genomic DNA is obtained, and the primer sequence of the 18S rDNA gene ITS region sequence is: ITS1 (SEQ:NO.2): 5'-TCCGTAGGTGAACCTGCGG-3'; ITS4 (SEQ: NO. 3): 5'-TCCTCCGCTTATTGATATGC-3'. After the amplification is completed, use agarose gel electrophoresis to detect the PCR products. figure 2 . The sequenced genomic DNA was put into blast alignment, and the results showed that HGC34 had the closest parental relationship with Meyerozyma guilliermondii (Pichia guilliermondii). The isolated strain was identified as Pichia jiemenii.

experiment example 2

[0037] Put the isolated Pichia moniliformes HGC34 into a test tube filled with fresh chicken manure as a base material, and carry out a laboratory test of bacterial strain application. Design three repetitions. The odor intensity grades are shown in Table 1:

[0038] Table 1 Odor evaluation grade

[0039]

[0040]

[0041]The cultured HGC34 bacteria deodorized seed solution (bacteria concentration of 8.0×10 9 CFU / mL) were inoculated into test bottles containing fresh chicken manure, and after fully stirring and mixing, they were placed in a constant temperature incubator at 30 °C for cultivation, and used as a blank control group (CK), and the amount of inoculum was observed every 10 days. The effect on deodorization performance is shown in Table 2.

[0042] Table 2 Effects of different inoculum amounts on deodorization

[0043]

[0044] As can be seen from Table 2, the deodorized seed solution of Pichia moniliformis HGC34 in the inoculation season is completely d...

experiment example 3

[0046] In the test tube of YPD liquid medium, inoculate the seed solution of HGC34 strain (the concentration of bacterial solution is 6.8×10) according to the inoculation amount of 3.0%. 8 CFU / mL), cultured at 30°C, 35°C, 40°C, 45°C, 50°C, 55°C, 60°C, and 65°C for 48h, respectively, and counted the number of yeast and dead and live cells by hemocytometer. The result is as Figure 4 As shown, HGC34 grows best at 55°C, indicating that this strain is a thermophilic strain. Pichia jiemenii HGC34 was observed under the microscope by methylene blue staining, and the cell shape was round or oval, transparent, slightly larger than bacteria. No flagella, sterile filaments, mostly white, a few blue, budding visible, with a smaller bud next to the larger parent cell (see Figure 5 ). Count the dead and live cells under the microscope, the dead cells are blue, and the live cells are colorless, and the proportion of dead and live cells can be calculated by counting. The results showed ...

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Abstract

The invention belongs to the technical field of microorganisms, and provides a high-temperature-resistant Meyeromyces guilliermondii strain HGC34 and application thereof in deodorization and degradation of livestock and poultry manure. The pichia guilliermondii HGC34 is preserved in the China Center for Type Culture Collection on October 31, 2016, and the preservation number of the pichia guilliermondii HGC34 is CCTCC (China Center for Type Culture Collection) NO: M2016600. The high-temperature-resistant pichia guilliermondii HGC34 disclosed by the invention has high-temperature resistance, and can be used for remarkably removing odor in livestock and poultry manure such as chicken manure, pig manure and the like, so that the aims of protecting the environment and promoting rapid degradation and decomposition of compost are fulfilled.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a high temperature-resistant Pichia moniliformes strain HGC34 and its application in the deodorization and degradation of livestock and poultry manure. Background technique [0002] With the rapid development of large-scale and facility-based domestic breeding industry, a large amount of waste is generated from livestock and poultry breeding, which brings serious environmental and social problems. Odor is one of the challenges faced by the centralized treatment of livestock manure. The main components of odor generated in the composting process include ammonia, hydrogen sulfide and volatile organic compounds (VOCs), which cause serious harm to the ecological environment. Diverse microbial flora in composting habitats play an important role in both odor generation and deodorization. Some microbial flora can generate odor by decomposing organic matter, and some microorganis...

Claims

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Application Information

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IPC IPC(8): C12N1/16C02F11/02C05F17/20C12R1/645C02F103/20
CPCC12N1/16C02F11/02C05F17/20C02F2103/20C02F2303/02
Inventor 郑永良龙丽芳王政徐碧林吕锐玲范霞刘畅
Owner HUANGGANG NORMAL UNIV
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