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Induction culture medium, culture method and application of callus of endangered semi-mangrove plant tung paulownia

A technology for inducing culture medium and mangrove plants, which is applied in the field of inducing medium for callus of endangered semi-mangrove plant A. sylvestris, and can solve the problem of not being suitable for callus culture of A. Problems such as low survival rate, to achieve the effect of low browning rate, high cure rate and low pollution rate

Active Publication Date: 2022-07-22
HAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the traditional plant callus induction medium and culture method existing in the prior art are not suitable for the culture of the callus of the lotus plant, which leads to the lower survival rate of the callus culture of the lotus plant, and provides Induction medium, culture method and application of a kind of callus of endangered semi-mangrove plant P.

Method used

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  • Induction culture medium, culture method and application of callus of endangered semi-mangrove plant tung paulownia
  • Induction culture medium, culture method and application of callus of endangered semi-mangrove plant tung paulownia
  • Induction culture medium, culture method and application of callus of endangered semi-mangrove plant tung paulownia

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Prepare induction medium: configure induction medium according to the following content components: the specific composition of induction medium is as follows: based on AA medium, add 0.1mg / L 6-BA, 0.5mg / LNAA, 28g / L Sucrose, 6.8 g / L agar; pH adjusted to 5.8. Then sterilize at 0.1Pa and 121°C constant temperature for 20min.

[0026] (2) Leaf pretreatment: Pick the 2nd and 3rd tender leaves at the top of A. chinensis, rinse them under running water, and soak them for 30s to remove surface dirt; soak the leaves in 70% ethanol for 30s under sterile conditions, without Bacterial water was rinsed 3 times, then disinfected with 0.1% HgCl2 solution for 15 min, rinsed 3 times with sterile water, put into a petri dish with sterile filter paper, and absorbed the liquid on the surface of the material.

[0027] (3) Inoculation of explants

[0028] All the leaf margins, main leaf veins and soaked wounds of the pretreated leaves were cut off, and cut into square leaf blocks of 5...

Embodiment 2

[0032] (1) Prepare induction medium: configure induction medium according to the following content components: the specific composition of induction medium is as follows: based on AA medium, add 1 mg / L 6-BA, 0.5 mg / LNAA, 28 g / L sucrose , 6.8g / L agar; adjust pH to 5.8. Then sterilize at 0.1Pa and 121°C constant temperature for 20min.

[0033] (2) Leaf pretreatment: Pick the 2nd and 3rd tender leaves at the top of A. chinensis, rinse them under running water, and soak them for 60s to remove surface dirt; soak the leaves in 70% ethanol for 45s under sterile conditions, without The bacteria water was rinsed 3 times, then disinfected with 0.1% HgCl2 solution for 16 min, rinsed 3 times with sterile water, put into a petri dish with sterile filter paper, and absorbed the liquid on the surface of the material.

[0034] (3) Inoculation of explants

[0035] All the leaf margins, main leaf veins and soaked wounds of the pretreated leaves were cut off, and cut into square leaf blocks of...

Embodiment 3

[0039] (1) Prepare induction medium: configure induction medium according to the following content components: the specific composition of induction medium is as follows: based on AA medium, add 0.5mg / L 6-BA, 0.5mg / LNAA, 28g / L Sucrose, 6.8 g / L agar; pH adjusted to 5.8. Then sterilize at 0.1Pa and 121°C constant temperature for 20min.

[0040] (2) Leaf pretreatment: Pick the 2nd and 3rd tender leaves at the top of A. chinensis, rinse them under running water, and soak them for 50s to remove surface dirt; soak the leaves in 70% ethanol for 40s under sterile conditions. Bacterial water was rinsed 3 times, then disinfected with 0.1% HgCl2 solution for 20 min, rinsed 3 times with sterile water, put into a petri dish with sterile filter paper, and absorbed the liquid on the surface of the material.

[0041] (3) Inoculation of explants

[0042] All the leaf margins, main leaf veins and soaked wounds of the pretreated leaves were cut off, and cut into square leaf blocks of 5 mm×5 mm...

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Abstract

The invention discloses an induction culture medium for callus of an endangered hemimangrove plant tung paulownia and application of the induction culture medium to tissue culture of the hemimangrove plant. The invention also discloses a culture method of the callus of the endangered semi-mangrove plant tung paulownia and application of the callus to tissue culture of the semi-mangrove plant. According to the induction culture medium and the culture method, the callus of the semi-mangrove plant paulownia fortunei can be efficiently cultured, the survival rate of callus culture is remarkably improved, the callus yield is high, the browning rate is low, the pollution rate is low, and technical support for seedling culture and propagation is provided for solving the endangered problem of the species.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to an inducing medium, a culture method and application of callus of the endangered semi-mangrove plant A. chinensis. Background technique [0002] Lithium alba is a semi-mangrove plant belonging to the genus Lithocarpus, which is often used for windbreak and sand fixation. It is an important component tree species for coastal protection and has important ecological protection value. Liu Liping, Wu Yinsheng and others conducted research and analysis on the components of the branches and seeds of A. chinensis, and found that they are rich in various substances such as lignans and alkaloids that resist cancer, tumors, leukemia and other diseases, so it also has important medicinal value. However, in the state of natural reproduction, the reproductive capacity of wild lotus leaf trees is extremely poor, and there is a situation of abortion. It has been unable to reproduce normal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/00Y02A40/22
Inventor 王勇倪靓于靖吴秋妍黄兰李燕华
Owner HAINAN NORMAL UNIV
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