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Prophylactic or ameliorating agent for periodontal disease

A periodontal disease and periodontal technology, applied in the field of periodontal disease prevention or improvement agent, can solve problems such as unclear function

Pending Publication Date: 2022-07-19
KAO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] On the other hand, it is known that Neisseria genus bacteria such as Neisseria siccus, Neisseria sicca, Neisseria flavinus, Neisseria flavinus, Neisseria flavinus, Neisseria longum, etc. The oral cavity exists as non-pathogenic commensal bacteria whose function is unclear

Method used

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  • Prophylactic or ameliorating agent for periodontal disease
  • Prophylactic or ameliorating agent for periodontal disease
  • Prophylactic or ameliorating agent for periodontal disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Example 1 The effect of Neisseria mucus in inhibiting the infection of periodontal pathogens (Porphyromonas gingivalis, Fusobacterium nucleatum, Treponema tartarus and Prevotella intermedia)

[0094] (1) Cell culture conditions

[0095] Human gingival epithelial cells (Ca9-22) were obtained from the JCRB Cell Bank (JCRB0625).

[0096] Use DMEM (Gibco, containing 10% v / v FBS, 1% v / v penicillin-streptomycin) at 37°C, CO 2 Ca9-22 was cultured at a concentration of 5%.

[0097] (2) Bacterial culture conditions

[0098] Porphyromonas gingivalis and Fusobacterium nucleatum were obtained from ATCC, Treponema denticola and Prevotella intermedia were obtained from the Microbial Materials Development Laboratory (JCM) (Table 1). Colony culture was performed using anaerobic Columbia medium (Japan Becton Dickinson (hereinafter referred to as Japan BD)), and liquid culture was performed using GAM broth medium (Nisui Pharmaceutical Co., Ltd., containing 5.0 μg / mL Hemin, 17.4 μg / mL ...

Embodiment 2

[0121] Example 2 Inhibitory effect of bacterial culture of Neisseria mucilage on infection of periodontal pathogens (Porphyromonas gingivalis, Fusobacterium nucleatum, Treponema denticola and Prevotella intermedia) (1) Neisseria mucus Preparation of bacterial cultures of S. cerevisiae

[0122] For Neisseria mucilage, 300 mL of BHI medium was used for culturing at a temperature of 37° C. for 48 hours to prepare a Neisseria mucilage culture solution.

[0123] Regarding the Porphyromonas gingivalis culture supernatant non-added group, GAM broth medium was added to the Neisseria mucilage culture medium so that the final concentration was 5%, and after culturing at a temperature of 37°C for 48 hours, 20 mL of the culture solution of serki was recovered in a 50 mL tube. Regarding the Porphyromonas gingivalis culture supernatant addition group, the prepared Porphyromonas gingivalis culture supernatant was added to the Neisseria mucus culture solution so that the final concentration ...

Embodiment 3

[0131] Example 3 Inhibitory effect of Neisseria mucus on alveolar bone resorption using mouse periodontal disease model mice

[0132] (1) Target animal

[0133] As the subject animal, 4-week-old female BALB / c mice (SLC, Japan) were used. Mice were reared under the conditions of room temperature 23±2°C, 55±10%, 12-hour circadian cycle (circadian rhythm: AM7-PM7). During the 1-week pre-feeding period, the mice drank tap water ad libitum and ate the animal chow CE-2 solid food (Clea, Japan) ad libitum.

[0134] (2) Bacterial culture conditions

[0135] Bacterial culture was carried out in the same manner as in Example 1 using Porphyromonas gingivalis, Neisseria mucus, and Neisseria pyogenes of the same strain as in Example 1.

[0136] (3) Porphyromonas gingivalis-infected mouse model of periodontitis

[0137] Referring to the method of kobayashi et al. (R. Kobayashi, et al. J Dent Res (2011) 90(5): 653-658), evaluation was performed using a model of periodontitis infected wit...

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Abstract

Provided is a material which suppresses infection caused by periodontal pathogens and is useful for preventing or ameliorating periodontal diseases. The periodontal pathogen infection inhibitor contains, as an active ingredient, a cell or a cell culture of Neisseria mucilaginosa.

Description

technical field [0001] The present invention relates to a preventive or ameliorating agent for periodontal disease. Background technique [0002] The cause of periodontal disease includes periodontal pathogenic bacteria that cause chronic inflammatory diseases among more than 600 kinds of bacteria present in the oral cavity. Periodontal pathogens infect periodontal tissue and invade into cells, causing gingivitis and periodontal tissue destruction. [0003] Existing techniques for preventing and improving periodontal disease include bactericides and antibiotics against periodontal pathogens. These techniques have strong bactericidal and bacteriostatic effects, and therefore may destroy normal oral flora. It can lead to the emergence of resistant bacteria, which can lead to serious diseases. [0004] In addition, in recent years, a technique for suppressing the growth of periodontal pathogenic bacteria has been reported using lactobacillus and bifidobacteria, which have bee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A23L33/135A61P1/02A61P31/04C12N1/20
CPCA23L33/135A61K35/74A61P1/02A61P31/04C12N1/20C12P19/04C12R2001/36
Inventor 赤津友基藤井明彦白石晶子山本尚基惣野初美
Owner KAO CORP
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