Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Periodontal pathogen infection marker detection kit and detection method

A technology for detecting reagents and pathogenic bacteria, applied in the field of biological and medical detection, can solve the problems of lack of guiding significance in the detection of infection markers and formulation of treatment measures, and achieve the effect of preventing the occurrence of AD and preventing the deterioration of the disease.

Active Publication Date: 2021-04-06
珠海市德灏生物科技有限公司
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009]Current laboratory tests for AD are limited to characteristic pathological markers, and no infection markers related to AD are detected, and the test results are only useful for the diagnosis of the disease Supporting effect, lack of guiding significance for the formulation of treatment measures such as the prevention of the disease and the control of the development of the disease

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Periodontal pathogen infection marker detection kit and detection method
  • Periodontal pathogen infection marker detection kit and detection method
  • Periodontal pathogen infection marker detection kit and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] The following reagents were prepared for use in the detection experiments in the various embodiments of the present invention.

[0076] 1. Preparation of sample eluent

[0077] Prepare sample eluent according to the following formula

[0078] MES 4.26g

[0079] Deionized water 100ml

[0080] Adjust the pH to 6.5 and store at 2-8°C for later use.

[0081] 2. Preparation of the microwell coating of the microplate and the corresponding chromogenic solution

[0082] Prepare the detection hole coating solution and the corresponding chromogenic solution as shown in Table 1:

[0083] Table 1

[0084]

[0085] Blank microplates, also known as reaction cards, can be purchased directly from the market. The microporous plate consists of a bottom plate and a plate cover. The bottom plate is provided with a plurality of grooves (ie, micropores) for placing filter paper sheets. Before use, filter paper sheets are placed on the bottom plate, and the filter paper sheets are us...

Embodiment 2

[0088] Example 2 Detection of quality control strain-Porphyromonas gingivalis strain gingival protease

[0089] 1. Strain expansion:

[0090] a) Positive strains: After recovery of the Porphyromonas gingivalis strain, transfer to the Columbia blood agar plate, place an anaerobic bag, and incubate at a constant temperature of 37°C for 5-7 days until black colonies appear on the surface of the medium;

[0091] b) Negative strains: After recovering the strains of Streptococcus A, transfer them to Columbia blood agar plates, and culture them at a constant temperature of 37°C for 24-48 hours until grass-green hemolytic colonies appear in the culture medium;

[0092] 2. Prepare 0.5 McFarland turbidity unit bacterial solution: Scrape the positive and negative strains into sterile saline, adjust the concentration to 0.5 McFarland turbidity unit;

[0093] 3. Prepare bacterial solutions with different concentrations: 0.5 McFarland turbidity unit is equivalent to 1.5*10 8 Calculate CFU...

Embodiment 3

[0097] Example 3 Detection of gingival protease quality control enzyme-trypsin

[0098] 1. Prepare concentrated quality control solution: prepare 256u / ml trypsin enzyme solution with sample eluent;

[0099] 2. Prepare quality control products of different concentrations: dilute the concentrated quality control solution with the sample eluent, and prepare trypsin enzyme concentrations of 256u / ml, 128u / ml, 64u / ml, 32u / ml, 16u / ml ml, 8u / ml, 4u / ml, 2u / ml, 1u / ml, 0.5u / ml quality control products;

[0100] 3. Use a single-item coated microwell plate coated with gingival protease detection well coating solution II for the test, and add concentrations of 64u / ml, 32u / ml, 16u / ml, and 8u / ml to the 1-9 wells respectively , 4u / ml, 2u / ml, 1u / ml, 0.5u / ml, 0u / ml quality control products 30ul, gingival protease detection hole coating solution II corresponding color solution 30ul;

[0101] 4. Place in a constant temperature incubator at 37°C for 20 minutes, and observe the color change of the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a periodontal pathogen infection marker detection kit, which comprises a sample eluent, a myeloperoxidase detection reagent and a gingival protease detection reagent, and is characterized in that the sample eluent is an aqueous solution of tris(hydroxymethyl) aminomethane, 3-morpholine propanesulfonic acid, 4-hydroxyethyl piperazine ethanesulfonic acid, piperazine-N, N'-bis (2-ethylsulfonic acid) or 2-(N-morpholine) ethanesulfonic acid; the myeloperoxidase detection reagent comprises oxidase, a substrate corresponding to the oxidase and a color developing agent A, hydrogen peroxide can be generated after the oxidase meets the substrate corresponding to the oxidase, and the color developing agent A comprises a Trinder reaction chromogen substrate; the gingival protease detection reagent is a gingival protease detection reagent I or II, the gingival protease detection reagent I comprises a substrate BAEE, ethanol oxidase and a color developing agent B, and the color developing agent B comprises peroxidase and a Trinder reaction chromogen substrate; and the gingival protease detection reagent II comprises BANA and a diazonium salt color developing agent.

Description

technical field [0001] The invention relates to the technical field of biological and medical detection, in particular to a detection kit and a detection method for infection markers of periodontal pathogenic bacteria. Background technique [0002] Alzheimer's disease (AD) is a degenerative disease of the central nervous system, accounting for 60-70% of senile dementias. The main clinical manifestations are neuropsychiatric symptoms such as progressive memory impairment, cognitive dysfunction, personality changes and language barriers. The characteristic pathological changes are extracellular senile plaques formed by the deposition of β-amyloid protein (Aβ), neurofibrillary tangles (NFTs) in nerve cells formed by hyperphosphorylation of tau protein, and neuron loss with glial cell proliferation. [0003] The exact etiology and pathogenesis of AD have not yet been fully elucidated, but it is agreed that the occurrence of AD is the result of the joint action of genetic factor...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/28C12Q1/37C12Q1/26
CPCC12Q1/28C12Q1/37C12Q1/26G01N2333/908G01N2333/902C12Q2326/12C12Q2326/10C12Q2326/30C12Q2326/32
Inventor 农高惠何林声
Owner 珠海市德灏生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com