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Acremonium persicae MR-47 and application thereof

A technology of Acremonium peach, MR-47, applied in application, climate change adaptation, chemicals for biological control, etc., can solve the problems of human health threats, ecological balance destruction, pesticide residues, etc.

Active Publication Date: 2022-06-03
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term use of chemical pesticides not only easily leads to pesticide residues, but also forms drug resistance, destroys ecological balance, causes environmental pollution and poses a threat to human health

Method used

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  • Acremonium persicae MR-47 and application thereof
  • Acremonium persicae MR-47 and application thereof
  • Acremonium persicae MR-47 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Separation, purification and screening of Acremonium peach MR-47

[0029] experimental method:

[0030] 1. The soil samples tested were collected by the five-point sampling method from the artificially cultivated windbreak rhizosphere soil collected from the Medicinal Botanical Garden of Jilin Agricultural University (43°48′23″N, 125°24′57″) in July 2019. Store in a sterile ziplock bag and store in a 4°C refrigerator for later use.

[0031] 2. Use the dilution coating method to separate the fungi in the soil, weigh 10 g of the wind-dried rhizosphere soil sample and put it into a conical flask containing 90 mL of sterile physiological saline, shake it for 30 minutes, and make the soil sample evenly dispersed It becomes soil suspension in the dilution solution; after the soil samples are dispersed, they are successively diluted by 10-fold gradient to obtain soil dilution solutions of various concentrations; respectively, take the dilution gradient as 10 -3 , 1...

Embodiment 2

[0035] Example 2 Identification of Acremonium peach MR-47

[0036] experimental method:

[0037] 1. Select the purified strain MR-47 and inoculate it on the PDA medium, cultivate it in the dark at 25°C for 14 days, and carry out morphological identification according to its colony morphology, hyphal diameter, spore size, morphology and implantation method, and refer to " The "Handbook of Fungal Identification" preliminarily determines the taxonomic status of strains.

[0038] 2. Use the kit TaKaRa MiniBEST Universal Genomic DNA Extraction Kit Ver.5.0 to extract the total genomic DNA of strain MR-47. The ITS rDNA of strain MR-47 was PCR amplified and sequenced using fungal universal primers ITS1 (5'-TCCGTAGGTGAACCTGCGG-3') and ITS4 (5'-TCCTCCGCTTATTGATATGC-3'). PCR reaction system: Master Mix 12.5 μL, ITS 11 μL, ITS 41 μL, rDNA 2 μL, ddH 2 O 8.5 μL, the amplification program was 94°C for 3 min; 94°C for 30s, 55°C for 30s, 72°C for 1min, 30 cycles; 72°C for 5min. PCR amplifi...

Embodiment 3

[0043] Embodiment 3 The antibacterial spectrum of Acremonium peach MR-47

[0044] experimental method:

[0045] Determination of Acremonium persicinum MR-47 against Fusarium oxysporum, Fusarium solani, Phytophthora cactorum, Botrytis cinerea, Rhizoctonia solani by plate confrontation method , the antibacterial spectrum of Alternaria tenuissima, Alternaria liriodendra, Sclerotinia sclerotiorum, Cylindrocarpon destructans, the specific operation steps are the same as step 3 in Example 1.

[0046] Experimental results:

[0047] Acremonium persicinum MR-47 has strong antibacterial effect on 9 pathogenic fungi, among which the inhibitory effect on Phytophthora mala and Cylindrosporium is the strongest, reaching more than 70%, 72.22% and 72.22% respectively. 74.07%; the bacteriostatic effect against Rhizoctonia solani is weak, only 40.74%; the bacteriostatic rate against several other fungi is 62-70%, indicating that Acremonium persicinum MR-47 has a relatively wide range of The ...

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Abstract

The invention discloses acremonium persicae MR-47 and an application thereof, and relates to the field of biological prevention and control, in particular to acremonium persicae MR-47 and an application of the acremonium persicae MR-47. The strain is preserved in Guangdong Microbial Culture Collection Center on January 14, 2022, and the preservation number is GDMCC No: 62209. The strain MR-47 has relatively strong antibacterial activity on a plurality of plant pathogens, and the antibacterial rate reaches 62-74%; the strain MR-47 can enable fusarium equisetum hyphae to expand, shrink, break, distort, deform and the like, antagonize the growth of fusarium equisetum and achieve the bacteriostasis rate of 60%; the strain MR-47 can obviously reduce the disease index of the divaricate saposhnikovia root rot, and the control effect reaches 75.05%; the bacterial strain MR-47 has relatively strong broad-spectrum antibacterial resistance and soil colonization capability; the strain MR-47 has certain biocontrol potential and growth promoting effect, and can be used for preparing green biopesticide for preventing and treating phytopathogen.

Description

Technical field [0001] The present invention relates to the technical field of biological control, and specifically relates to an Acremonium persicinum strain MR-47 and its application. Background technique [0002] Traditional Chinese medicinal materials are an important material basis for the development of traditional Chinese medicine. my country is the world's largest producer of traditional Chinese medicinal materials with the largest scale, most varieties, and the most complete production system. During the cultivation and production of Chinese medicinal materials, pests and diseases, excessive pesticide residues, and illegal use of hormones are important issues affecting the yield and quality of Chinese medicinal materials and have attracted much attention. [0003] Biological control has the characteristics of green, safe, sustainable, broad-spectrum, and non-polluting. The development and application of biocontrol bacteria have become a hot spot in the prevention an...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P3/00A01P21/00C12R1/645
CPCA01N63/30Y02A40/10Y02A50/30
Inventor 赵淑杰战杨韩忠明高俊文王云贺王妍杨利民韩梅杜立财
Owner JILIN AGRICULTURAL UNIV
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