Antithrombin III determination kit

A technology of antithrombin and kits, which is applied in biological testing, measuring devices, instruments, etc., can solve the problems of large cost investment, multiple reagent components, complex production process and operation, etc., to achieve improved accuracy and specific reaction Sexual enhancement, enhanced sensitivity effects

Pending Publication Date: 2022-05-27
WUHAN CHANGLI BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the commercially available a

Method used

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  • Antithrombin III determination kit
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  • Antithrombin III determination kit

Examples

Experimental program
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Effect test

Embodiment 1

[0061] 1.1 The antithrombin III assay kit of the present invention includes:

[0062] Reagent 1:

[0063] The buffer was HEPES buffer, pH 6.5, and the concentration of the buffer was 100 mM.

[0064] The surfactants are Tween 20 and Triton X-100, Tween 20 accounts for 0.02wt% of the buffer weight, and Triton X-100 accounts for 0.10wt% of the buffer weight;

[0065] The reaction modifiers are polyethylene glycol 6000, potassium chloride, sodium sulfate, polyethylene glycol 6000 accounts for 2.00wt% of the buffer weight, potassium chloride accounts for 0.05wt% of the buffer weight, and sodium sulfate accounts for 0.05wt% of the buffer weight. 0.60wt%;

[0066] The anti-interference agents are potassium ferrocyanide and heterophilic antibody blocking agent, potassium ferrocyanide accounts for 0.5wt% of the buffer weight, and the heterophilic antibody blocking agent accounts for 0.02wt% of the buffer weight;

[0067] The preservatives are gentamycin sulfate and sodium azide, ge...

Embodiment 2

[0103] Embodiment 2 verifies the effect of the kit of the present invention

[0104] 2.1 Uniformity test

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Abstract

The invention belongs to the field of in-vitro detection kits, and particularly relates to an antithrombin III determination kit. The antithrombin III determination kit comprises a reagent 1 and a reagent 2, the reagent 1 comprises a buffer solution, a surfactant, a reaction regulator, an anti-interference agent and a preservative, and the reagent 2 comprises a buffer solution, salt ions, a protein protective agent, a surfactant, a preservative and an antithrombin III antibody. The reagent has the beneficial effects that the uniformity of the reagent is improved by adding the surfactant, and the reaction specificity of the reagent for determining a target object is enhanced by adding the anti-interference agent, so that the accuracy of a reagent determination result is improved; by adding the reaction regulator, the sensitivity of the reagent and the accuracy of a sample determination result are enhanced; by optimizing the formulas of the protein protective agent and the preservative, the stability of the reagent is improved, the reagent can be stored for a long time, and the production cost is reduced.

Description

technical field [0001] The invention belongs to the field of in vitro detection kits, in particular to an antithrombin III detection kit. Background technique [0002] Antithrombin III is mainly synthesized in hepatocytes and endothelial cells. It is a single-chain glycoprotein composed of 432 amino acid residues with a relative molecular mass of 58200KD. Antithrombin III is a member of the serine protease inhibitor superfamily. It mainly exists in two structures, α-type and β-type. They both contain 3 β-sheets, 9 α-helices and 1 RCL, and have similar three-dimensional structures and conformations. An important difference between antithrombin III and serine protease inhibitors is that antithrombin III can enhance its inhibitory activity by binding to heparin-like molecules. [0003] Antithrombin III is an inhibitor of thrombin and coagulation factors XIIα, XIα, IXα, Xα and other proteases. It combines with thrombin through the arginine-serine peptide bond to form an antith...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68G01N2333/8128
Inventor 毕德熙陈莹郑文果毕少辉沈亚楠
Owner WUHAN CHANGLI BIOLOGICAL TECH CO LTD
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