Dual-mode fluorescent copper nanocluster for rapid fluorescence detection of trinitrotoluene or trinitrophenol

A technology of fluorescent copper nanoclusters and trinitrotoluene, applied in fluorescence/phosphorescence, measuring devices, and material analysis through optical means, can solve the complex synthesis steps and post-processing of organic fluorescent probes, which do not meet the requirements of on-site detection, At the same time, problems such as difficult detection are achieved to achieve the effect of abundant copper source, easy access to copper source and low price

Pending Publication Date: 2022-05-24
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Although the fluorescent materials currently used to detect trinitrotoluene (TNT) and trinitrophenol (TNP) can produce specific fluorescent signal changes to the target, the response is fast and the sensitivity is high, but due to the similar structure of TNT and TNP Its electron-deficiency ability is similar, which brings some difficulties to the simultaneous detection of the two.
A related patent (CN 111943907A) reported that trinitrotoluene (TNT) and trinitrophenol (TNP) can be effectively distinguished by using the different degrees of fluorescence intensity quenching and the different degrees of red shift of emission peaks before and after the reaction with fluorescent probes. Technical means, but organic fluorescent probes require complex synthesis steps and post-processing, and this method requires further confirmation by fluorescence spectrometers, which does not meet the needs of on-site detection

Method used

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  • Dual-mode fluorescent copper nanocluster for rapid fluorescence detection of trinitrotoluene or trinitrophenol
  • Dual-mode fluorescent copper nanocluster for rapid fluorescence detection of trinitrotoluene or trinitrophenol
  • Dual-mode fluorescent copper nanocluster for rapid fluorescence detection of trinitrotoluene or trinitrophenol

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Experimental program
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Effect test

Embodiment 1

[0032] a. At room temperature, dissolve 100 mg of branched polyethyleneimine (PEI) Mw=3500 in 4 mL of ultrapure water, and stir uniformly for 10 min;

[0033] b. Add 200 μL of copper sulfate with a concentration of 100 mM and 1 mL of ascorbic acid with a concentration of 500 mM into the branched polyethyleneimine solution in step a, stir evenly, and react at a temperature of 40 °C for 2 hours, the final solution turns light green and appears under an ultraviolet lamp Bright green fluorescence was obtained, indicating that dual-mode fluorescent copper nanoclusters PEI-Cu NCs were obtained;

[0034]c. Put 200 μL of the dual-mode fluorescent copper nanoclusters obtained in step b into a quartz cuvette container, then add 1M NaOH to adjust the pH to 10.0, add 200 μL of trinitrotoluene methanol solution, and use a fluorescence spectrometer after 15 minutes. Scan the fluorescence spectra before and after TNT addition, such as figure 1 As shown in the figure, it can be seen that aft...

Embodiment 2

[0036] a. At room temperature, dissolve 100 mg of branched polyethyleneimine (PEI) Mw=3500 in 4 mL of ultrapure water, and stir uniformly for 10 min;

[0037] b. Add 200 μL of copper sulfate with a concentration of 150 mM and 1 mL of ascorbic acid with a concentration of 600 mM into the branched polyethyleneimine solution in step a, stir evenly, and react at a temperature of 40 °C for 2 hours. The final solution turns light green and appears under ultraviolet light. Bright green fluorescence was obtained, indicating that dual-mode fluorescent copper nanoclusters PEI-Cu NCs were obtained;

[0038] c. Place 200 μL of the dual-mode fluorescent copper nanoclusters obtained in step b in a quartz cuvette container, add 1M NaOH to adjust the pH to 10.0, and then add 200 μL of trinitrotoluene (TNT) methanol solution, after 15min, in Significant quenching of the green fluorescence of copper clusters was observed under 365 nm UV light.

Embodiment 3

[0040] a. At room temperature, dissolve 100 mg of branched polyethyleneimine (PEI) Mw=3500 in 4 mL of ultrapure water, and stir uniformly for 10 min;

[0041] b. Add 200 μL of copper sulfate with a concentration of 200 mM and 1 mL of ascorbic acid with a concentration of 700 mM into the branched polyethyleneimine solution in step a, and stir evenly, react at a temperature of 40 °C for 2 hours, and the final solution turns light green and appears under an ultraviolet lamp Bright green fluorescence was obtained, indicating that dual-mode fluorescent copper nanoclusters PEI-Cu NCs were obtained;

[0042] c. Put 200 μL of the dual-mode fluorescent copper nanoclusters obtained in step b into a test tube, add 1M NaOH to adjust the pH to 10.0, and then add 200 μL of trinitrophenol (TNP) methanol solution, and scan with a fluorescence spectrometer after 15 minutes The fluorescence spectra before and after the addition of TNT showed that after the reaction, the fluorescence emission pe...

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Abstract

The invention provides a dual-mode fluorescent copper nano-cluster for rapid fluorescence detection of trinitrotoluene or trinitrophenol, and the selectivity and sensitivity of the fluorescent copper nano-cluster to nitro explosive molecules are researched by using two channels, namely an ultraviolet-visible light absorption spectrum and a fluorescence spectrum. The recognition mechanism is deeply analyzed by combining theoretical calculation and single-photon counting fluorescence lifetime measurement, and the result shows that after trinitrotoluene (TNT) or trinitrophenol (TNP) with the same concentration is added, the emission peak of the nano-cluster at 500 nm generates strong fluorescence quenching, the quenching effect on TNP is better, and the recognition accuracy is higher. In addition, a new absorption peak can be generated at 475 nm only when trinitrotoluene (TNT) is added, and the color becomes red. After the copper nano-cluster is in contact with TNT or TNP, naked eye visible fluorescence quenching and colorimetric phenomena can be achieved within 1 min, and complicated instruments and equipment are not needed, so that low-cost real-time field detection is realized.

Description

technical field [0001] The invention relates to a dual-mode fluorescent copper nano-cluster for fast fluorescent detection of trinitrotoluene or trinitrophenol. Background technique [0002] In recent years, explosives have seriously threatened people's life and property safety, and they are also major environmental pollutants, seriously endangering human health. Therefore, it is of great practical and strategic significance to carry out research on explosive detection. Among various detection techniques, fluorescence sensing has been used to detect TNT in vapor, solution and solid due to its advantages of remote operation, high sensitivity and low cost. Since Swager et al. first used organic conjugated polymer thin films to detect trace explosives in 1998, the research of fluorescence sensing technology in the detection of trace explosives has been rapidly developed and applied, and new fluorescent probes have been reported continuously. Detection of trinitrotoluene (TNT)...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432G01N2021/6439
Inventor 窦新存吴昊天王广发蔡珍珍
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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