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Method for detecting pesticide residues based on ratio fluorescence and colorimetric dual modes of ACP (at) Ce/Tb-IPA

A technology of pesticide residues and ratiometric fluorescence, applied in the field of analytical chemistry, can solve the problems of single output signal, expensive detection equipment, complex operation of detection methods, etc.

Active Publication Date: 2022-05-20
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010](1) Some detection methods are relatively complicated to operate, and the detection equipment is relatively expensive;
[0011](2) Some detection methods only have a single output signal, and the accuracy cannot be verified

Method used

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  • Method for detecting pesticide residues based on ratio fluorescence and colorimetric dual modes of ACP (at) Ce/Tb-IPA
  • Method for detecting pesticide residues based on ratio fluorescence and colorimetric dual modes of ACP (at) Ce/Tb-IPA
  • Method for detecting pesticide residues based on ratio fluorescence and colorimetric dual modes of ACP (at) Ce/Tb-IPA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] 1. the amount of substance is 0.50mmol of ceric ammonium nitrate (Ce(NH 4 ) 2 (NO 3 ) 6 ) and terbium nitrate hexahydrate (Tb(NO 3 ) 3 ·6H 2 O) be dissolved in 10mL deionized water and ultrasonically stirred to form a yellow homogeneous solution A;

[0050] 2. Pour solution A into 40mL N,N-dimethylformamide solution containing isophthalic acid (IPA, 100mM), and stir for 20min to form yellow homogeneous solution B;

[0051] 3. Pour solution B into the reaction kettle, heat and react in a blast oven at 150°C for 5 hours; after the product is naturally cooled, centrifuge at a speed of 10,000r / min for 10 minutes to separate the product, wash it with ethanol, and dry it in vacuum at 50°C for 12 hours;

[0052] 4. Take 100 mg of the dried reaction product, add 600 μL of ACP solution (20 mg / mL) and 1 mL of triethylamine to mix in 20 mL of deionized water, after ultrasonic treatment for 10 min, stir magnetically at low temperature for 12 h, centrifuge to collect the solid...

Embodiment 2

[0057] 1. Dissolve 10 mg of the prepared ACP@Ce / Tb-IPA in 10 mL of ultrapure water, and stir ultrasonically to form a yellow uniform solution;

[0058] 2. Take 920 μL of NaAc-HAc solutions with pH values ​​of 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, and 6.0, respectively, add 50 μL of 10 mM TMB and 50 μL of 1 mg / mL ACP@Ce / Tb-IPA to disperse Solution, mixed evenly, reacted at room temperature for 11 minutes, the color of the liquid changed from colorless to blue with time;

[0059] 3. Measure the absorbance with an ultraviolet-visible absorption spectrophotometer;

[0060] The result is as figure 2 As shown in A, as the pH increases, the absorbance value first increases and then decreases, and when the pH is controlled at 4.0, the absorbance (652nm) value is the highest and reaches a peak. It can be seen from the figure that when the pH is controlled at 4.0, the catalytic activity of the enzyme is the best;

[0061] 4. Take 920μL of NaAc-HAc solution (0.2M, pH 4.0), add 30μL of 10mM T...

Embodiment 3

[0063] 1. Dissolve 10 mg of the prepared ACP@Ce / Tb-IPA in 10 mL of ultrapure water, and stir ultrasonically to form a yellow uniform solution;

[0064] 2. Take 920μL NaAc-HAc solution (0.2M, pH 4.0), add 50μL 1mg / mL ACP@Ce / Tb-IPA dispersion solution to it in turn, mix well, measure the fluorescence spectrum of the above mixed solution with a fluorescence spectrophotometer .

[0065] Depend on image 3 It can be seen that there are two fluorescent signals at 358nm and 496nm.

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Abstract

The invention belongs to the technical field of analytical chemistry, and relates to a method for detecting pesticide residues based on ratio fluorescence and colorimetric dual modes of ACP (at) Ce / Tb-IPA, which comprises the following steps: adding 50 [mu] L of 1mg / mL ACP (at) Ce / Tb-IPA dispersion liquid into a centrifuge tube, adding organophosphorus pesticide paraoxon with different concentrations, and incubating for 30 min; continuously adding 930 [mu] L of a NaAc-HAc solution and 20 [mu] L of an AAP solution, measuring fluorescence, and drawing a standard working curve taking the paraoxon concentration as a horizontal coordinate and the emission wavelength intensity ratio at 496 nm to 358 nm as a vertical coordinate; adding 30 [mu] L of a TMB solution into the mixed system, measuring the absorbance, and drawing a standard working curve with the paraoxon concentration as the abscissa and the absorbance at 652 nm as the ordinate; after the sample to be detected is treated in the same way, the detected numerical value is compared with the working curve, and the concentration can be obtained. According to the present invention, by using the specific triggering inhibition of the ACP activity and the potential influence of the by-product on the ACP (at) Ce / Tb-IPA oxidase and the luminescence characteristic, the dual-mode indirect detection of the pesticide residue is achieved, and the huge application potential is provided in the field of the pesticide residue detection.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry and relates to a method for detecting pesticide residues, in particular to a method for detecting pesticide residues based on ACP@Ce / Tb-IPA dual-mode ratiometric fluorescence and colorimetry. Background technique [0002] Pesticides play an important role in ensuring crop yields, but their long-term use in large quantities not only easily leads to resistance of pests and diseases, but also pesticide residues will continue to accumulate in crops, soil and wastewater. In fact, only about 1% of the pesticides really play a role, and the rest enter the natural environment, accumulate in a large amount through the food chain and remain for a long time, and finally cause harm to human health, leading to acute poisoning, carcinogenesis and various diseases, and even affect the human body. Therefore, it is of great significance to establish a pesticide residue detection method with simple ope...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/31G01N21/78
CPCG01N21/643G01N21/31G01N21/78G01N2021/6421
Inventor 牛湘衡刘朋汪梦珠
Owner JIANGSU UNIV
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