Microbial agent for preventing both root knot nematode disease and clubroot and preparation method thereof
A microbial agent and technology of clubworm, applied in the field of fertilizers, can solve the problems of difficulty in timely prevention and control, difficulty in quickly distinguishing root-knot nematode disease from clubroot disease, and simultaneous occurrence of root-knot nematode disease and clubroot disease. Promote growth, improve crop resistance, and improve the effect of absorption and utilization
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0040] The present embodiment provides a kind of microbial inoculum for preventing root-knot nematode and clubroot, and the specific preparation method is as follows:
[0041] The first step: preparing methylotrophic bacillus bacteria powder and bacillus subtilis bacteria powder
[0042] The methylotrophic Bacillus and Bacillus subtilis preserved in glycerol were taken out from -80°C, inoculated into liquid LB medium at a ratio of 3‰, and cultured with shaking at 38°C for 24 hours to obtain the strains of the two strains. seed liquid. The activated seed liquid is inoculated into the first-level medium according to the ratio of 10% for cultivation to obtain the first-level seed liquid, and then according to the proportion of 60% of the volume, the first-level seed liquid is respectively inoculated into the second-level medium for cultivation, Obtain secondary seed solution. Next, the secondary seed liquids were respectively introduced into disc centrifuges, centrifuged at a r...
Embodiment 2
[0056] The present embodiment provides a kind of microbial inoculum for preventing root-knot nematode and clubroot, and the specific preparation method is as follows:
[0057] The first step: preparing methylotrophic bacillus bacteria powder and bacillus subtilis bacteria powder.
[0058]The methylotrophic Bacillus and Bacillus subtilis preserved in glycerol were taken out from the condition of -80°C, respectively inoculated into the liquid LB medium according to the ratio of 5‰, and the two strains were obtained after shaking culture at 35°C for 48 hours. seed liquid. The activated seed liquid is inoculated into the first-level medium according to the proportion of 5% for cultivation to obtain the first-level seed liquid, and then the first-level seed liquid is respectively inoculated into the second-level medium according to the proportion of 70% by volume for cultivation, Obtain secondary seed solution. Next, the secondary seed liquids were respectively introduced into di...
Embodiment 3
[0072] The present embodiment provides a kind of microbial inoculum for preventing root-knot nematode and clubroot, and the specific preparation method is as follows:
[0073] The first step: preparing methylotrophic bacillus bacteria powder and bacillus subtilis bacteria powder.
[0074] The methylotrophic Bacillus and Bacillus subtilis preserved in glycerol were taken out from -80℃, inoculated into liquid LB medium at a ratio of 4‰, and cultured with shaking at 37℃ for 35 hours to obtain the two strains. seed liquid. The activated seed liquid is inoculated into the primary medium according to the ratio of 8% for cultivation to obtain the primary seed liquid, and then the primary seed liquid is respectively inoculated into the secondary medium according to the volume of 65% for cultivation, Obtain secondary seed solution. Next, the secondary seed liquids were respectively introduced into disc centrifuges, centrifuged at a rotational speed of 6000r / min for 15min, and the sup...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com