Lactococcus lactis MD-622 and application thereof

A technology of MD-622 and Lactococcus lactis, applied in the field of microorganisms, can solve the problems that Lactococcus lactis has not been recognized and utilized, and achieve the effect of improving intestinal health and promoting growth and development

Pending Publication Date: 2022-05-13
MEI YI TIAN BIOLOGICAL MEDICINE WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many actual types of Lactococcus lactis, and many Lactococcus lactis and their functions have not been recognized and utilized

Method used

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  • Lactococcus lactis MD-622 and application thereof
  • Lactococcus lactis MD-622 and application thereof
  • Lactococcus lactis MD-622 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 screening lactic acid bacteria

[0024] Take the feces of healthy adults around 25 years old, dilute them with distilled water, spread them on MRS medium (lactic acid bacteria medium), culture them upside down at 37°C, select single colonies with different shapes on the medium for streaking, continue to purify and culture, and separate The strains numbered 5b4m2, cb18m1, PS128, XS10M8, XS14M1, XS14M5 were obtained.

Embodiment 2

[0025] Embodiment 2 produces enzyme activity test

[0026] The enzyme production activity tests were carried out on the strains 5b4m2, cb18m1, PS128, XS10M8, XS14M1, and XS14M5 respectively:

[0027] (1) Inoculate the above strains into protease screening medium containing 10g / L skimmed milk powder, 10g / L peptone, and 15g / L agar powder, respectively, and culture them at 37°C. The production of protease in the culture medium was further detected by the protease hydrolysis circle method.

[0028] The statistical results are shown in the table below:

[0029] Statistical table of protease production by different bacteria (N=3)

[0030]

[0031]

[0032] (2) Inoculate the above strains with 13mM Tris-HCL, 75mM NaCl, 1.3mM CaCl 2 (pH8.0), agar 15g / L, 10mg / L rhodamine B, polyethylene olive oil emulsion 31.25mL, cultured at 37°C in a lipase screening medium. The production of lipase in the culture medium was further detected by the lipase transparent circle method.

[0033...

Embodiment 3

[0053] Embodiment 3 Toxicity test and safety detection

[0054] The cb18m1 strain was submitted for inspection for toxicological tests and safety testing. The results showed that the culture of the cb18m1 strain would not endanger human health or animal or plant pathogens or pollute the environment.

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PUM

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Abstract

The invention provides a lactobacillus lactis MD-622 and an application thereof. The lactobacillus lactis MD-622 can promote the production of protease, amylase and cellulase, and has the potential capability of promoting the digestion of human intestinal tracts. In addition, the lactobacillus lactis MD-622 disclosed by the invention can also promote the growth and development of embryonic stem cells to a certain extent.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a Lactococcus lactis MD-622 and an application thereof. Background technique [0002] Lactococcus lactis (Lactococcus lactis, L.lactis) is a prokaryotic microorganism, belonging to Firmicutes, Bacillus, Lactobacillus, Streptococcus, Lactococcus genus, and is an important model bacteria in the genus Lactobacillus. [0003] L.lactis widely exists in dairy products and plant products, and is widely used in the food industry. It is non-pathogenic to humans and animals, and is a food-grade microorganism generally regarded as safe (GRAS). However, there are many actual types of Lactococcus lactis, and many Lactococcus lactis and their functions have not been recognized and utilized. Contents of the invention [0004] Based on this, it is necessary to provide a Lactococcus lactis MD-622 and its application. The Lactococcus lactis MD-622 can promote the production of protease, amylas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/744A61P1/00A23L33/135C12R1/01
CPCC12N1/20A61K35/744A61P1/00A23L33/135A23V2002/00A23V2400/231A23V2200/3204
Inventor 刘庆军沈鹤霄张帆李国龙刘慧敏彭文聪熊云王峰
Owner MEI YI TIAN BIOLOGICAL MEDICINE WUHAN CO LTD
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