Kit and test method for detecting catecholamine intermediate metabolite in human plasma

Abstract

The invention provides a kit and a test method for detecting catecholamine intermediate metabolites in human plasma, and the kit comprises the following reagents: (1) a calibrator solution, which is divided into calibrators with six concentrations, the calibrator with each concentration contains three catecholamine intermediate metabolites: methoxyepinephrine, methoxynoradrenaline and 3-methoxytyramine, and the calibrator solution is a solution of calibrators with six concentrations; the concentration of the calibration product is 10 pg / mL to 4000 pg / mL; (2) quality control products including a quality control product QCL, a quality control product QCM and a quality control product QCH; (3) an internal standard solution, namely a methanol solution mixed solution containing intermediate metabolites of three catecholamines; (4) a buffer solution I and a buffer solution II; (5) preparing a derivative solution; (6) leacheate; (7) an eluent; (8) a 96-hole purification plate and a 96-hole receiving plate; and (9) covering a 96-well plate with a film. The sample is treated by solid-phase extraction and then derivatization reaction, so that the detection sensitivity of the catecholamine intermediate metabolite in the sample is improved.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a kit and a test method for detecting intermediate metabolites of human plasma catecholamines. Background technique [0002] Catecholamine (CA), also known as catecholamine, mainly includes epinephrine (Epinephrine, referred to as E), norepinephrine (Norepinephrine, referred to as NE) and dopamine (Dopamine, referred to as DA). Catecholamine is a monoamine neurotransmitter synthesized and secreted by the adrenal medulla, adrenal neurons, and extra-adrenal chromaffin bodies. It is an endogenous substance with strong physiological activity and plays an important role in brain and nerve signal transduction. It not only plays an extensive role in regulating the physiological activities of the human body's cardiovascular system, nervous system, endocrine system, kidney, smooth muscle tissue, etc., but also affects the body's metabolism at the same time. The intermediat...

AI Technical Summary

Problems solved by technology

[0005] The detection of catecholamines and metabolites has gone through a variety of methods, and these traditional methods have their inherent shortcomings: bioanalysis, colorimetry, low sensitivity; fluorescence method, easily interfered by other endogenous compounds; radioenzyme method, The operation is cumbersome; the detection sensitivity of the chemiluminescence method cannot meet the needs of clinical testing; while the liquid chromatography coupled with electrochemistry (LC-ECD) has high sensitivity and selectivity, but it takes a long time and is susceptible to interference from various substances

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