Preparation method and application of stem cell extract

A technology of extracts and stem cells, applied in the field of preparation of stem cell extracts, can solve the problems of low survival rate of stem cells, inconvenience and pain of patients, local infection, etc., and achieve the effect of easy aseptic treatment, convenient use, and avoiding invasive operations

Pending Publication Date: 2022-05-10
陈传果
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in previous clinical studies, live stem cells were transplanted into the body through microneedles or injections, which easily caused local infection, and the survival rate of stem cells was low. To achieve a good curative effect, repeated injections were required, bringing patients inconvenience and pain

Method used

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  • Preparation method and application of stem cell extract
  • Preparation method and application of stem cell extract
  • Preparation method and application of stem cell extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 A kind of stem cell extract preparation method, comprises the following steps:

[0026] 1. The materials, reagents, instruments, etc. used in the examples can be obtained from commercial sources unless otherwise specified.

[0027] 2. Isolation and Identification of Umbilical Cord Mesenchymal Stem Cells

[0028] 1) Obtain a 30 cm umbilical cord from a healthy puerpera without knowing it. After collection in the operating room, soak it in sterile PBS and transport it to the laboratory. Soak it in 75% alcohol for 1 minute and rinse it, then put it on a 10 cm plate, rinse it with sterile PBS 3 times.

[0029] 2) Cut the umbilical cord into 5 cm pieces, tear the umbilical cord with tweezers and carefully remove the blood vessels (including 2 arteries and 1 vein), and carefully tear the umbilical cord into filaments.

[0030] 3) Transfer the filaments to a 50 mL centrifuge tube, add 10 mL of PBS and mix well, then insert surgical scissors into the centrifuge t...

Embodiment 2

[0042] Embodiment 2 A method for preparing stem cell extracts, comprising the following steps:

[0043] 1. The materials, reagents, instruments, etc. used in the examples can be obtained from commercial sources unless otherwise specified.

[0044] 2. Isolation and Characterization of Placental Mesenchymal Stem Cells

[0045] 1) The placenta was obtained from healthy puerpera under the condition of informed knowledge. After collection in the operating room, it was soaked in sterile PBS and transported to the laboratory. After being soaked in 75% alcohol for 1 minute and rinsed, it was rinsed three times with sterile PBS.

[0046] 2) Carefully peel off the placental decidua and tear it into thin strips with scissors and forceps.

[0047] 3) Transfer the thin strips of tissue to a 50 mL centrifuge tube, add 10 mL of PBS and mix well, then insert surgical scissors into the centrifuge tube for quick cutting.

[0048] 4) Add 10 mL of 0.1% type I collagenase to the centrifuge tube,...

Embodiment 3

[0061] Example 3 Stem cell extracts for hair loss

[0062] 1. Patient grouping. 20 patients with hair loss were divided into two groups, 10 people in the experimental group and 10 people in the placebo group, using the stem cell extract spray prepared in Example 1 and placebo saline respectively.

[0063] 2. How to use: Spray the placebo or stem cell extract on the hair loss area, 2 times a day, for 16 weeks.

[0064] 3. Effect evaluation, every 8 weeks to evaluate the effect of patients, including hair density, side effects. Hair density was assessed on a 7-point scale: -3, significantly decreased; -2, moderately decreased; -1, slightly decreased; 0, no change; 1, slightly increased; 2, moderately increased; 3, markedly increased. Side effects include itching, redness and swelling.

[0065] 4. Analysis of the results of use. During the 8 weeks of use, one person in the test group developed itching at the site of use, and no allergies occurred in the placebo group. Use a h...

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Abstract

The invention relates to the technical field of biology, in particular to a preparation method and application of a stem cell extract. Based on the repair and regeneration capacity of stem cells, the invention provides a preparation process of the stem cell extract, the preparation method is simple, the use is convenient, and the stem cell extract has great application potential in treatment of alopecia and diabetic wounds.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method and application of a stem cell extract. Background technique [0002] Stem cells have the characteristics of self-renewal and differentiation, and are universal seed cells for tissue engineering, which can secrete a variety of growth factors (HGF, VEGF, IGF, PDGF) to promote tissue regeneration and repair. At the same time, due to the low immunogenicity of stem cells, they have excellent biocompatibility during allogeneic transplantation, and have a large number of clinical applications in the treatment of hair loss and diabetic wounds. [0003] Alopecia patients include androgenetic alopecia, oily alopecia, and pathological alopecia. Androgenetic alopecia (AGA) is the most common type of hair loss. Among people over the age of 50, 50% of men and 45% of women will experience AGA to varying degrees. Although hair loss does not endanger people's life safety, it w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775C12N1/06A61K35/28A61P17/14A61P17/08A61P17/02A61P3/10
CPCC12N5/0662C12N1/066A61K35/28A61P17/14A61P17/08A61P17/02A61P3/10C12N2509/00
Inventor 陈传果
Owner 陈传果
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