Nitrogen-assisted microbial system as well as preparation method and application thereof
A technology of microorganisms and functional microorganisms, applied in chemical instruments and methods, mining fluids, earth drilling and production, etc., can solve problems such as injection pressure differences, foam system composition not proposed, injection volume can not be effectively controlled, etc., to achieve input-output ratio High, good oil-increasing effect, long-term effect
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[0026] According to the second aspect of the present invention, the present invention discloses a method for preparing the above-mentioned nitrogen-assisted microbial system. The specific steps of the method are as follows:
[0027] (1) Mixing functional microorganisms, biosurfactants and biopolymers uniformly to obtain a mixture of biological compound systems;
[0028] (2) Simultaneously inject the above-mentioned biological complex system mixture pressurized by the plunger pump and the nitrogen gas pressurized by the gas booster pump into the ground foaming equipment to form a nitrogen-assisted microbial system.
[0029] Preferably, the boost pressure of the biological compound system mixture is greater than 5 MPa, and the boost pressure of the gas booster pump is greater than 5 MPa.
[0030] Preferably, the pressure resistance value of the floor foaming equipment is greater than 6MPa, and the temperature resistance value is greater than 100°C.
[0031] According to the thi...
Embodiment 1
[0046] (1) uniformly mix Geobacillus, rhamnolipid and biological polysaccharide according to the mass ratio of 1:0.10:0.05 to obtain the mixture of biological compound system;
[0047] (2) The above-mentioned biological complex system mixture pressurized to 6.5MPa by the plunger pump and the nitrogen gas pressurized to 7.5MPa by the gas booster pump are simultaneously injected into the ground foaming equipment to form nitrogen-assisted microbial system A.
[0048] The liquid-to-gas volume ratio of the biological complex system to nitrogen is 1:2.
Embodiment 2
[0050] (1) uniformly mixing Bacillus paralicheniformis, rhamnolipid and xanthan gum according to the mass ratio of 1:0.15:0.07 to obtain the mixture of the biological compound system;
[0051] (2) Simultaneously inject the above biological complex system mixture pressurized to 7.0MPa by the plunger pump and nitrogen gas pressurized to 8.0MPa by the gas booster pump into the ground foaming equipment to form the nitrogen-assisted microbial system B.
[0052] The liquid-to-gas volume ratio of the biological complex system to nitrogen is 1:3.
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