Nano-drug carrier wrapped by bacterial outer membrane vesicles and preparation method of nano-drug carrier

A nano-drug carrier, outer membrane vesicle technology, applied in nano-drug, nano-technology, nano-technology and other directions, can solve problems such as poor stability, reduce toxicity, increase intestinal absorption, and reduce normal organ damage.

Pending Publication Date: 2022-04-26
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the pH sensitivity of PDA in the stomach, this structure also has the disadvantage of poor stability

Method used

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  • Nano-drug carrier wrapped by bacterial outer membrane vesicles and preparation method of nano-drug carrier
  • Nano-drug carrier wrapped by bacterial outer membrane vesicles and preparation method of nano-drug carrier
  • Nano-drug carrier wrapped by bacterial outer membrane vesicles and preparation method of nano-drug carrier

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Embodiment 1

[0035] (1) Preparation of nano-drug carriers wrapped in bacterial outer membrane vesicles

[0036] Proceed as follows:

[0037] 1. Preparation of mesoporous silica nanoparticles

[0038] Weigh 0.534g cetyltrimethylammonium bromide (CTAB) and dissolve it in 240mL deionized water, stir to dissolve, add 1.25mL 2M NaOH and stir again for 5min, keep the reaction solution at 80°C for 30min at a constant speed, Then dropwise add a total of 5mL tetraethyl orthosilicate (TEOS) to it at a speed of 10 μL / 4 seconds, fully react for 2 hours after the dropwise addition, and then carry out centrifugation (5000rpm, 20min) to get the precipitate, which is the MSN crude product; Wash the crude MSN with methanol and deionized water alternately, repeat 3 times, and dry overnight in a vacuum oven at 30°C to obtain the crude MSN without removing the template agent CTAB; place the crude MSN without removing the template agent CTAB in the muffle In the furnace, the temperature was raised to 550°C a...

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Abstract

The invention relates to a nano-drug carrier wrapped by bacterial outer membrane vesicles and a preparation method of the nano-drug carrier. The carrier adopts outer membrane vesicles secreted by Escherichia coli to wrap polydopamine modified doxorubicin-loaded mesoporous silica nanoparticles, and the preparation method comprises the following steps: preparing the doxorubicin-loaded mesoporous silica nanoparticles, modifying the doxorubicin-loaded mesoporous silica nanoparticles by polydopamine, and extracting and purifying the outer membrane vesicles of bacteria. And preparing the nano-drug carrier OMVs-NPs wrapped by the outer membrane vesicles of the bacteria. The prepared OMVs-NPs can prevent the medicine from being released too early in a biological environment, outer membrane vesicles secreted by Escherichia coli have an adsorption effect on intestinal tracts, the OMVs-NPs serve as a biological outer membrane of a novel nano preparation, intestinal absorption of medicine carrying nanoparticles can be improved, the medicine is released after tumor cells are targeted, and the bioavailability of the medicine is improved.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and in particular relates to a nano drug carrier wrapped by bacterial outer membrane vesicles and a preparation method thereof. Background technique [0002] Oral administration of cancer is widely used in clinical practice for long-term administration due to its advantages of simple operation, low production cost, and no damage to local skin or mucous membranes during use. However, it is also limited by multiple absorption barriers in the gastrointestinal tract, liver clearance, Narrow therapeutic index and some drugs are limited by factors such as poor solubility, poor stability, or poor penetration. In order to improve the shortcomings of traditional oral drug delivery, novel nano drug delivery systems have emerged, and significant progress has been made in improving the bioavailability of drugs. [0003] Mesoporous silica nanoparticles (MSN) have become a widely used novel nanocarr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/46A61K47/69A61K47/59A61K31/704A61P35/00B82Y5/00
CPCA61K9/5176A61K47/6923A61K47/59A61K31/704A61P35/00B82Y5/00
Inventor 陈立江王泽雨石金燕王丽虹
Owner LIAONING UNIVERSITY
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