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Microbial agent capable of rapidly decolorizing aniline blue as well as preparation method and application of microbial agent

A microbial agent, aniline blue technology, applied in the field of microbial engineering, can solve the problems of incomplete removal, slow reaction speed, poor treatment effect, etc., achieve good decolorization effect, low cost, and reduce the effect of chemical reagents

Pending Publication Date: 2022-04-22
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a microbial bacterial agent used for rapid decolorization of aniline blue in dye wastewater to solve the problems of incomplete removal, poor treatment effect and slow reaction speed in the treatment of aniline blue in dye wastewater

Method used

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  • Microbial agent capable of rapidly decolorizing aniline blue as well as preparation method and application of microbial agent
  • Microbial agent capable of rapidly decolorizing aniline blue as well as preparation method and application of microbial agent
  • Microbial agent capable of rapidly decolorizing aniline blue as well as preparation method and application of microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The preparation of embodiment 1 microbial bacterial agent

[0020] Include the following steps:

[0021] (1) Comamonas testosteroni CT1 (Comamonas testosteroni ATCC11996), KF1 (Comamonas testosteroni ATCC49202), Acinetobacter calcoaceticus LM1 (Acinetobacter calcoaceticus), Pseudomonas stutzeri JP1 (Pseudomonas sp.) from -80 Take it out of the refrigerator at ℃, thaw at room temperature, add LB high-salt medium, and culture in shake flask at 37°C and 160r / min overnight to complete the activation of bacteria;

[0022] (2) Subculture the above-mentioned activated strain with LB high-salt medium, and adjust the OD of the bacteria 600 = 1.0, the same amount is prepared as a mixed bacterial solution; the mixed bacterial solution is concentrated at low temperature, the concentration volume is 4:1, the concentration temperature is -18°C, and the number of viable bacteria in the bacterial agent is obtained by counting method is about 2.85×10 12 CFU / g;

[0023] The LB high-sa...

Embodiment 2

[0024] The preparation of embodiment 2 microbial bacterial agents

[0025] Include the following steps:

[0026] (1) The same amount of Comamonas testosteroni CT1 (Comamonas testosteroni ATCC11996), KF1 (Comamonas testosteroni ATCC49202), Acinetobacter calcium acetate LM1 (Acinetobacter calcoaceticus), Pseudomonas stutzeri JP1 (Pseudomonas sp.) were obtained from Take it out of the refrigerator at -80°C, thaw at room temperature, add LB high-salt medium, and culture overnight at 37°C and 180r / min in a shaker flask to complete the activation of the bacteria;

[0027] (2) Subculture the above-mentioned activated strain with LB high-salt medium, and adjust the OD of the bacteria 600 = 1.0, the same amount is prepared as a mixed bacterial solution; the mixed bacterial solution is concentrated at low temperature, the concentration volume is 5:1, the concentration temperature is -20°C, and the number of viable bacteria in the bacterial agent is obtained by counting method is about ...

Embodiment 3

[0029] The preparation of embodiment 3 microbial bacterial agents

[0030] Include the following steps:

[0031] (1) The same amount of Comamonas testosteroni CT1 (Comamonas testosteroni ATCC11996), KF1 (Comamonas testosteroni ATCC49202), Acinetobacter calcium acetate LM1 (Acinetobacter calcoaceticus), Pseudomonas stutzeri JP1 (Pseudomonas sp.) were obtained from Take it out of the refrigerator at -80°C, thaw at room temperature, add LB high-salt medium, and culture overnight at 37°C and 200r / min in a shaker flask to complete the activation of the bacteria;

[0032] (2) Subculture the above-mentioned activated strain with LB high-salt medium, and adjust the OD of the bacteria 600 = 1.0, the same amount is prepared as a mixed bacterial solution; the mixed bacterial solution is concentrated at low temperature, the concentration volume is 6:1, the concentration temperature is -19°C, and the number of viable bacteria in the bacterial agent is obtained by counting method is about ...

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Abstract

The invention relates to a microbial agent capable of rapidly decolorizing aniline blue as well as a preparation method and application thereof, and belongs to the technical field of microbial engineering. The comamonas testosteroni strain is prepared from comamonas testosteroni CT1, KF1, acinetobacter calcoaceticus LM1 and pseudomonas stutzeri JP1 which are equal in quantity. The method has the beneficial effects that the environmental adaptability and aniline blue decoloring efficiency of the strain are improved through the synergistic symbiotic effect of various microorganisms, the optimal degradation condition suitable for the strain is changed, the degradation capacity of the strain and the degradation efficiency of the triphenylmethane dye aniline blue are improved, and the ecological and human health risks caused by environmental pollution are effectively reduced; the method has the advantages that the method is simple, the decolorization rate reaches 86.0%, the decolorization effect is good, the cost is low, the operation is simple, the degradation efficiency is high, the environmental compatibility is good, the use of chemical reagents is reduced, the secondary pollution is avoided, and the method has a good application prospect in the aspect of bioremediation of the aniline blue polluted environment.

Description

technical field [0001] The invention belongs to the technical field of microbial engineering, and in particular relates to a microbial bacterial agent capable of rapidly decolorizing aniline blue, a preparation method thereof and an application in the decolorization treatment of dye wastewater. Background technique [0002] In many industries such as textiles, papermaking, leather, rubber, plastics, etc., dyes are widely used as colorants for products. At present, there are more than 100,000 kinds of dyes on the market in our country, with an annual production of more than 7,105 tons, resulting in a considerable amount of colored wastewater. Since color is the first pollutant to be identified in wastewater, the public's perception of water quality is largely influenced by color, and even a very small amount of dye can be highly visible and uncomfortable. Azo and anthraquinone dyes are the third most used dyes, including malachite green, crystal violet, brilliant green and a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/38C02F101/30
CPCC12N1/20C02F3/34C02F2101/308
Inventor 郭立泉闻增昕王佳琦任苡萱马传福
Owner JILIN AGRICULTURAL UNIV
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