Kit for determination of trimethylamine oxide, preparation method and application thereof

Abstract

The invention provides a kit for measuring trimethylamine oxide, a preparation method and application thereof, the kit provided by the invention can effectively solve the current situation of poor stability of the kit prepared by the solution process, and can realize the freezing of the internal standard. Dry and long term stability. The kit includes a series of trimethylamine oxide calibrators and deuterated trimethylamine oxide internal standards; the series of trimethylamine oxide calibrators are lyophilized products of a series of trimethylamine oxide calibrator working solutions with a concentration gradient, and the series of trimethylamine oxide calibrators are lyophilized. The trimethylamine oxide calibrator working solution is a trimethylamine oxide calibrator working solution with a concentration gradient obtained by diluting the known concentration of the trimethylamine oxide standard solution with the matrix solution; the deuterated trimethylamine oxide internal standard is known The lyophilized product of the deuterated trimethylamine oxide internal standard working solution with a predetermined concentration obtained by diluting the deuterated trimethylamine oxide standard solution with the concentration of the matrix solution; the matrix in the matrix solution is serum albumin.

Description

technical field [0001] The invention relates to the technical field of detection of endogenous biological markers, in particular to a kit for determining trimethylamine oxide, a preparation method and application thereof. Background technique [0002] As an endogenous biomarker, trimethylamine oxide (TMAO), like other biomarkers, needs to resolve matrix interference first to ensure quantitative accuracy. Therefore, when choosing a suitable matrix, developers often take 2 main ways: [0003] 1) Subtract the zero-concentration blank blood matrix, that is, select mixed blood as the matrix, subtract the calibrators and quality control substances of different concentrations from the zero-concentration mixed blood matrix, and remove the TMAO interference inherent in the mixed blood matrix. Curve fitting and assay of samples. Although this approach ensures the "original taste" of the matrix, it ignores the fact that there are significant differences in TMAO content between sample...

AI Technical Summary

Problems solved by technology

Although this approach ensures the “original taste” of the matrix, it ignores the fact that there are significant differences in TMAO content among samples, and the fact that mixed matrixes also have batch-to-batch inconsistencies, coupled with the fact that the source of mixed human blood matrix and ethics are restricted. Many limitations, so it is difficult to apply to the industrialization of the development of in vitro diagnostic kits (IVD)

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