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Quick hemostatic freeze-dried fiber aerogel as well as preparation method and application thereof

An airgel and freeze-drying technology, which is applied in applications, pharmaceutical formulations, surgery, etc., can solve the problems of hemostatic gel easy to fall off, poor effect, slow absorption of blood on the surface of the tissue and tissue adhesion, etc., to achieve a significant coagulation effect, The preparation process is simple and the hemostatic effect is remarkable

Active Publication Date: 2022-04-19
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, rapid hemostasis cannot be achieved by blood coagulation because of the time required for clot formation, especially in patients with anticoagulation or coagulation dysfunction
Therefore, the hemostatic gauze or hemostatic gel currently sold on the market or used in medical treatment can only slowly absorb blood and tissue adhesion on the surface of the tissue; moreover, the hemostatic gel is very easy to fall off after caking on the surface of the tissue Cause secondary bleeding and other problems, the hemostatic effect and speed need to be further improved

Method used

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  • Quick hemostatic freeze-dried fiber aerogel as well as preparation method and application thereof
  • Quick hemostatic freeze-dried fiber aerogel as well as preparation method and application thereof
  • Quick hemostatic freeze-dried fiber aerogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The rapid hemostasis freeze-dried fiber aerogel of the present invention is composed of 30-80% by weight of natural-based biological material, 10-60% by weight of fibrinogen and 2-10% by weight of cross-linked Combination composition. Wherein, the cross-linking agent is a mixture of NHS and EDC, the NHS is the abbreviation of the general chemical name of N-hydroxysuccinimide, and EDC is 1-ethyl-3-(3-dimethylaminopropyl) carbon two Abbreviation for the chemical name of imine hydrochloride.

[0025] The natural-based biological material is a biological material with a carboxyl group. In order to ensure the use effect, the natural-based biological material can preferably be made of one or more mixtures of chitosan, carboxymethyl chitosan and oxidized regenerated cellulose. accomplish. When using several mixtures of chitosan, carboxymethyl chitosan and oxidized regenerated cellulose, the weight percentages between the above materials can be arbitrarily configured. The re...

Embodiment 2

[0033] This embodiment takes the preparation of 10 g of rapid hemostasis freeze-dried fibrous aerogel as an example to illustrate the preparation method and steps.

[0034] First, weigh 5 g of chitosan, 4 g of fibrinogen, 0.89 g of EDC and 0.11 g of NHS, and mix them well; Stir at room temperature for 20 minutes to form a mixed solution; finally, introduce the mixed solution into a mold, freeze and freeze-dried to obtain the rapid hemostasis freeze-dried fiber aerogel.

[0035] When using, cut a 2X2mm gap on the surface of the mouse liver, dissolve the prothrombin with a concentration of 1wt% with a calcium chloride aqueous solution with a concentration of 1wt%, and apply the dissolved prothrombin to the wound surface of the organ, and then quickly The prepared aerogel material was attached to the wound surface and pressed continuously, and the liver wound of the mouse could quickly stop bleeding within 1.2 minutes.

Embodiment 3

[0037] This embodiment takes the preparation of 10 g of rapid hemostasis freeze-dried fibrous aerogel as an example to illustrate the preparation method and steps.

[0038] First, weigh 4.8 g of oxidized regenerated cellulose, 5.0 g of fibrinogen, 0.18 g of EDC and 0.02 g of NHS, and mix them well; Stir at room temperature for 20 minutes to form a mixed solution; finally, introduce the mixed solution into a mold, freeze and freeze-dried to obtain the rapid hemostasis freeze-dried fiber aerogel.

[0039] When using, cut a 2X2mm gap on the surface of the mouse liver, dissolve the prothrombin with a concentration of 1 wt% with a calcium chloride aqueous solution with a concentration of 1.5wt%, and coat the dissolved prothrombin on the wound surface of the organ, then The prepared aerogel material was quickly attached to the wound surface and pressed continuously, and the liver wound of the mouse could quickly stop bleeding within 3.2 minutes.

[0040] The following table shows t...

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Abstract

The invention discloses a quick hemostasis freeze-dried fiber aerogel which is characterized by comprising the following substances in percentage by weight: 30-80% of a natural-based biological material, 10-60% of fibrinogen and 2-10% of a cross-linking agent. Meanwhile, the invention also discloses a preparation method of the quick hemostasis freeze-dried fiber aerogel, which comprises the following steps: fully mixing and stirring 30-80% by weight of natural-based biological material, 10-60% by weight of fibrinogen and 2-10% by weight of cross-linking agent; and dissolving the mixture in a buffer solution with the pH value of 4.2-8.5, and fully stirring to form a mixed solution, and the like. According to the invention, the fibrinogen (pig source or human source) is creatively loaded together by using the catalyst and the cross-linking agent, the hydrogel is formed in situ, and then the hydrogel is prepared in a freeze-drying manner, so that not only is the preparation process simple, but also the blood coagulation effect is remarkable.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, in particular to a rapid hemostasis freeze-dried fiber aerogel and a preparation method and application thereof. Background technique [0002] It is well known that hemorrhage related to tissues and organs can be life-threatening, and uncontrolled massive hemorrhage is one of the leading causes of human death in the world. According to authoritative statistics, more than two million people die every year due to hemorrhage worldwide. Among the numerous causes of major bleeding, organ hemorrhage caused by surgical resection accounts for a large proportion, and surgical resection is very challenging to treat due to its high timeliness and complexity. In order to facilitate rapid hemostasis, most of the existing external hemostatic agents achieve hemostasis by accelerating intrinsic blood coagulation, that is, by rapidly absorbing water and concentrating coagulation factors. [0003] However, due ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/08A61L24/10A61L24/00
CPCA61L24/106A61L24/08A61L24/001A61L2400/04C08L89/00
Inventor 杨家印查湘军吕涛何莹
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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