PCR (Polymerase Chain Reaction) amplification instrument and amplification method for nucleic acid on-site variable-temperature amplification

Abstract

The invention relates to the technical field of PCR (polymerase chain reaction) gene amplification, and discloses a PCR amplification instrument and an amplification method for nucleic acid on-site variable-temperature amplification, the PCR amplification instrument comprises a shell, a cover, a sample placing groove, at least three sample placing plates, a tube groove and a sample tube placed in the tube groove, and the sample placing plates are sequentially arranged in the sample placing groove at intervals from the position close to the cover to the position away from the cover; a heating pipe is arranged in the sample placing plate, the sample placing plate is coated with a heat insulation layer, and the sample pipe comprises a pipe body, a pipe cover, a breather pipe which is arranged on the pipe body close to the pipe cover and enables an inner cavity of the pipe body to be communicated with the outside, and a first sealing piece which is arranged in the pipe body in a sealing and lifting manner; according to the PCR amplification instrument for nucleic acid amplification and the amplification method, the three steps of denaturation, annealing and extension are achieved in the same amplification instrument, meanwhile, repeated heating and cooling are not needed, the amplification time is shortened, temperature overshoot is avoided, and the amplification efficiency is improved. And the quality of gene amplification is ensured.

Description

technical field [0001] The invention relates to the technical field of PCR gene amplification, in particular to a PCR amplification instrument and an amplification method for on-site temperature-variable amplification of nucleic acids. Background technique [0002] The basic principle of PCR amplification is: double-stranded DNA will denature and form a single strand at a high temperature of 95°C in vitro, and artificially designed primer pairs will bind to The upstream or downstream of the two single strands; according to the primer design principle, the region between the upstream primer and the downstream primer of the primer pair is the target DNA fragment that needs to be extended, and then adjust the temperature to the reaction temperature of DNA polymerase, generally 72 ℃, to perform primer extension; repeat the three steps of denaturation, annealing and extension to achieve exponential replication of the target DNA fragment. PCR amplification instrument is an instrum...

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Problems solved by technology

[0004] The PCR gene amplification instrument puts the sample tube in the test tube tank, closes the tank cover, heats up the sample tank to 95°C to denature the sample, then cools down to about 55°C for annealing, and then heats up to 72°C for extension. In order to achieve amplification, but if repeated heating and cooling in the same lofting tank, it takes a long time, and overshooting phenomenon is prone to occur when the temperature changes, which affects the quality of the amplified gene

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