In-vitro culture and proliferation method for human mesenchymal stem cells
A bone marrow mesenchymal and in vitro culture technology, applied in the direction of bone/connective tissue cells, animal cells, vertebrate cells, etc., can solve the problems of low purity of stem cell growth, weak cell viability, large cell damage, etc., to achieve simple and easy operation Easy to use, simple method, strong effect of cell viability
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[0033] The present invention will be described in detail below with reference to the accompanying drawings and in combination with embodiments.
[0034] refer to Figure 4 As shown, the specific implementation of this embodiment is as follows:
[0035] 1) Preparation of bone marrow mesenchymal stem cell culture medium, the steps include:
[0036] a) Configure 950ml DMEM medium as the basic medium; b) Add 300mg L-glutamine, 50mg vitamin C, 15mU insulin, 10U erythropoietin and 10μg human stromal cell-derived factor-1 in turn; c) Add sodium hydroxide Adjust the pH to 7.0-7.2; d) Add 50ml of fetal bovine serum, filter and sterilize, and store at 4°C for later use.
[0037] 2) Cell culture
[0038] The primary cells of bone marrow mesenchymal stem cells were collected, digested with 0.25% trypsin to make a cell suspension, counted the number of cells, and adjusted the cell density to 2×105 cells / ml inoculated to the medium containing bone marrow mesenchymal stem cells; inoculate...
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