Micro-fluidic chip for sorting single cells and detection method

Abstract

The invention relates to a micro-fluidic chip for sorting single cells, which comprises a substrate layer, the upper surface of the substrate layer is provided with a first metal electrode pair, a second metal electrode pair and an impedance identification area, the impedance identification area comprises a first impedance detection electrode pair and a second impedance detection electrode pair, the first impedance detection electrode pair is connected with the first metal electrode pair, and the second impedance detection electrode pair is connected with the second metal electrode pair; the second impedance detection electrode pair is connected with the second metal electrode pair; the micro-channel layer is positioned above the substrate layer, and the micro-channel layer comprises a micro-fluidic pipeline and a sorting electrode; the microfluidic pipeline comprises a main channel, one end of the main channel is communicated with a sample inlet through a plurality of filter columns, the other end of the main channel is communicated with a sorting port, the sorting port is respectively communicated with a waste liquid channel and a collecting channel, the waste liquid channel is communicated with a waste liquid outlet, and the collecting channel is communicated with a target outlet; the sorting electrode comprises an excitation electrode and a ground electrode surrounding the excitation electrode, and a part of the excitation electrode and a part of the ground electrode are both close to the sorting opening.

Description

technical field [0001] The invention relates to the technical field of cell detection and analysis chips, in particular to a microfluidic chip for sorting single cells and a detection method, and more specifically to a microfluidic chip for label-free flow electrical impedance spectroscopy analysis and dielectrophoretic sorting of single cells Chips and detection methods. Background technique [0002] In life sciences and medicine, cell sorting and characterization techniques allow rapid isolation of desired subpopulations, identification and monitoring for clinical diagnosis. The recent rise of personalized medicine has highlighted the importance of single-cell analysis. For example, understanding the heterogeneity of a patient's solid tumor at the single-cell level could enable the possibility of targeting multiple cell subtypes to improve survival. [0003] Current single-cell analysis methods include flow cytometry and magnetic-activated cell sorting, but both of these ...

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Problems solved by technology

[0003]Current single cell analysis methods include flow cytometry and magnetic activated cell sorting, but both methods have shortcomings: (1) The sample preparation process is complicated and cumbersome , leading to potential loss of critical cells; (2) multiplexing of cell markers is limited by spectral overlap of markers; (3) requires a large number of cells

Patent 201110324803.9 proposes a similar method, but this patent has three defects: 1. Regarding the dielectric identification part, although the patent proposes to use multiple pairs of electrodes for multi-point frequency complex impedance measurement, in fact, multiple pairs of electrodes can also provide Flow rate measurement improves the accuracy of real-time sorting; 2. Regarding the analysis and processing of impedance information, the patent uses the pre-stored reference information of the complex dielectric constant of cells as the threshold for sorting signal generation, but each or even each cell object The physiological state of the place varies widely, and real-time specific sorting cannot be achieved only by a single value; 3. The impedance detection electrode is in contact with the solution, and the electrode will electrochemically react with the solution, resulting in measurement deviation and certain damage to the cells

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