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Application of riluzole in treatment of oligospermia

A technology of riluzole and oligospermia, which is applied in the field of pharmacy, can solve the problems of riluzole oligospermia treatment, etc., and achieve the effect of low cost, complete restoration and less side effects

Pending Publication Date: 2022-04-15
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the application of riluzole in the treatment of oligospermia

Method used

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  • Application of riluzole in treatment of oligospermia
  • Application of riluzole in treatment of oligospermia
  • Application of riluzole in treatment of oligospermia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1: Experimental Animal Modeling and Dosing Scheme

[0059] (1) Experimental animal feeding

[0060] Fifty 4-week-old Kunming male mice were purchased from Guangdong Experimental Animal Center, and were divided into 10 cages (5 mice / cage). During the feeding or experiment period, the room temperature was controlled at 25±1°C, the light and dark cycle was 12 hours of light, 12 hours of darkness, and the humidity was 50-60%. The mice were free to eat and drink. Follow-up experiments were carried out three days after the mice acclimatized to the environment.

[0061] (2) Grouping of experimental animals

[0062] Grouping situation:

[0063]

[0064] (3) Modeling of experimental animals

[0065] A single intraperitoneal injection of busulfan (30 mg / kg) was used in Kunming mice. The average weight of the mice was 27 g. Each mouse was injected with 0.8 mg of busulfan, and a total of 40 models were made.

[0066] Busulfan solution preparation:

[0067] ①Weigh 4...

Embodiment 2

[0074] Example 2: Analysis of experimental animal semen

[0075] Cut off the left epididymis tail of the mouse and put it in 1ml of PBS preheated to 37°C, and at the same time manually cut the tissue, and incubate in a 37°C water bath for 10 minutes to fully release the sperm. Mix the sperm suspension, draw 10 μL and add it to a mouse-specific sperm counting board with a depth of 60 μm, capture at least 30 fields of view for each mouse, and use a fully automatic sperm analyzer (CASA) (ML-810JZ, Nanning Songjing Tianlun Biotechnology Co., Ltd. Co., Ltd.) to comprehensively evaluate parameters such as sperm concentration, motility rate, motility, and deformity rate. The semen analysis of the CASA system showed that compared with the model group, intraperitoneal injection or oral administration of riluzole both increased the number of sperm in the model mice ( figure 1 and Figure 8 ), promote sperm regeneration, improve sperm viability and vitality, and significantly reduce th...

Embodiment 3

[0076] Example 3: HE staining of testis and epididymis paraffin sections

[0077] (1) Dewaxing to water: After baking slices at 60°C for 1 hour, soak in xylene for 15 min x 2 times, absolute ethanol for 5 min, 95% ethanol for 5 min, 80% ethanol for 5 min, 70% ethanol for 5 min, PBS for 5 min x 3 times.

[0078] (2) Hematoxylin staining: Take out the section and shake it dry, add hematoxylin staining solution to cover the tissue, stain for 3 minutes, then directly rinse the section with tap water for 2 to 3 times, then immerse the section in ultrapure water, observe the coloring under the microscope, the nucleus is blue-purple .

[0079] (3) Differentiation: The differentiation droplet is on the slice, 5-6s. Rinse with ultrapure water when the color fades.

[0080] (4) Anti-blue: Hematoxylin anti-blue droplets are on the slice, 5-6s. Rinse with ultrapure water when the color turns blue.

[0081] (5) Eosin staining: shake off the water stains on the slices, add eosin stainin...

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Abstract

The invention provides application of a non-hormone compound riluzole in treatment of oligospermia, provides beneficial help for solving the problem of male infertility, and has potential application prospects in the field of medicines.

Description

technical field [0001] The invention relates to the field of pharmacy, more specifically, the invention relates to the application of riluzole or its prodrug in the treatment of oligospermia. Background technique [0002] Infertility affects about 15% of couples worldwide, and about 50% of infertility is caused by men. With the aggravation of competitive pressure in modern society, and the intensification of environmental pollution such as radiation, smog, and water quality deterioration, the incidence of male infertility is increasing day by day, which has seriously affected family harmony and quality of life. It has been reported that among infertile men, 10-15% of them have extremely low concentration of sperm in their semen, that is, suffer from severe oligospermia (SO). Oligospermia is one of the most serious forms of male infertility, and the patient mainly has less than 5 million sperm / ml in the ejaculate due to failure of spermatogenesis. Studies have shown that ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/428A61P15/08
CPCA61K31/428A61P15/08A61K45/06
Inventor 杨艳黄亚东黄茹菲
Owner JINAN UNIVERSITY
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