Filler composition, injection comprising same, and method for injecting same
A technology of composition and injection, which is applied in the field of filler composition for oral tissue repair, can solve the problems of low success rate, unsightly appearance, and insufficient, etc., and achieve the effects of increasing the use time, optimizing bone configuration, and promoting bone formation
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[0113] In order to confirm the effect of the concentration change of hyaluronic acid (comparative example) and hyaluronic acid-polydeoxyribonucleotide (example) on the viability (proliferation rate) of human fibroblasts (HS68), 2% hyaluronic acid The acid solution was diluted in Dulbecco's modified Eagle's medium (supplemented with 1% (v / v) penicillin / streptomycin (Lonza, USA) and 10% (v / v) Fetal bovine serum (Lonza, New Jersey, U.S.) Dulbecco's (S) modified Eagle (S) medium (Korea Welgene (Welgene) company)), respectively prepared into 0.005, 0.01, 0.02, 0.05, 0.1, 0.2, 0.5% (v / v) concentrations, 2% (v / v) hyaluronic acid-polydeoxyribonucleotides were also diluted to the same concentrations as above. Cells were divided into 96-well plates (SPL Life Sciences, Korea) at a density of 5000 cells / cm2. For cell attachment, after culturing the cells for 24 hours, the medium was removed and 100ul was added to each well. Diluted hyaluronic acid and hyaluronic acid-polydeoxyribonucleot...
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