Biological paper chip, high-throughput multi-joint detection microwell plate device, preparation method and kit for multi-joint detection of vagina inflammation

A paper chip and microplate technology, used in biochemical equipment and methods, biochemical cleaning devices, enzymology/microbiology devices, etc., can solve problems such as reducing detection sensitivity, cumbersome sample addition process, and increasing missed detection rate. , to reduce the misjudgment rate, simplify the detection procedure, and improve the speed

Pending Publication Date: 2022-04-12
GENETEL PHARMA SHENZHEN
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In actual use, the sampling process of related technologies is cumbersome, and the sample dilution is large and the loss is large, which easily reduces the detection sensitivity and leads to an increase in the missed detection rate.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biological paper chip, high-throughput multi-joint detection microwell plate device, preparation method and kit for multi-joint detection of vagina inflammation
  • Biological paper chip, high-throughput multi-joint detection microwell plate device, preparation method and kit for multi-joint detection of vagina inflammation
  • Biological paper chip, high-throughput multi-joint detection microwell plate device, preparation method and kit for multi-joint detection of vagina inflammation

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0058] Detection zone substrate layer:

[0059] Hydrogen peroxide detection substrate: weigh 0.5g oxidase, add deionized water to prepare 0.5g / L oxidase solution; weigh 5g of 4-aminoantipyrine, add deionized water to prepare 5g / L 4-aminoantipyrine; Weigh 10g of 2,4,6-tribromo-3-hydroxybenzoic acid, add deionized water to prepare 10g / L of 2,4,6-tribromo-3-hydroxybenzoic acid Formic acid; 0.5g / L of oxidase solution, 5g / L of 4-aminoantipyrine and 10g / L of 2,4,6-tribromo-3-hydroxybenzoic acid were mixed uniformly to obtain hydrogen peroxide detection substrate.

[0060] pH detection substrate: weigh 1g bromocresol green, add deionized water to prepare 1g / L bromocresol green solution;

[0061] Sialidase detection substrate: Weigh 2g of 5-bromo-4-chloro-3-indole neuraminic acid salt, add deionized water to prepare 2g / L 5-bromo-4-chloro-3-indole neuraminic acid Amino acid salt solution; Weigh 10g 2-methoxyl-4-morpholine diazobenzene zinc chloride and add deionized water to prepare...

preparation example 2

[0068] Substrate layer in positioning area: weigh 1g methyl violet, add deionized water to prepare 1g / L methyl violet solution;

Embodiment 1

[0071] A method for preparing a high-throughput multiple detection microporous device, comprising the following steps:

[0072] Using Adobe Illustrator software on the S1 computer to design the pattern of the hydrophilic area and the hydrophobic area of ​​the bio-paper chip, the hydrophilic area includes a circular detection area with a diameter of 1.5 mm, and the distance between two adjacent detection areas is 0.5 mm, in a matrix distribution There are 9 detection areas in 3×3, and the detection area in the third row and third column is used as the positioning area;

[0073] S2 Print the pattern obtained in step S1 on an A4-size blank filter paper through a Xerox ColorQube8870 wax spray printer matrix to obtain a blank paper chip, wherein the blank filter paper is WhatmanTM No. 1 filter paper, and the length and width of each blank paper chip are 6.4×6.4mm;

[0074] S3 Place the blank paper chip obtained in step S2 in a metal bath at 100°C for 2 minutes to completely melt th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of biological detection, and particularly discloses a biological paper chip, a high-throughput multi-joint detection microwell plate device, a preparation method and a kit for multi-joint detection of vagina inflammation. The biological paper chip comprises a hydrophilic area and a hydrophobic area, and the hydrophilic area comprises a positioning area and a plurality of detection areas; the hydrophobic area is arranged around the positioning area and the plurality of detection areas and forms a hydrophobic boundary; a detection index substrate layer is arranged on each detection area, an indication layer is arranged on the positioning area, and the indication layer is used for indicating the position of the detection area. The biological paper chip can be used for being combined with a porous microplate to form a high-flux multi-union detection microplate device, and has the advantages of high-flux multi-union detection of multiple indexes, high detection efficiency, low sample dilution amount and high detection rate; in addition, the preparation method of the high-flux multi-union detection microwell plate device has the advantages of being simple in preparation and suitable for industrial mass production.

Description

technical field [0001] The present application relates to the field of biological detection, more specifically, it relates to a biological paper chip, a high-throughput multiple detection microwell plate device, a preparation method and a kit for multiple detection of vaginal inflammation. Background technique [0002] Ecological changes of microbial communities in the vaginal environment are closely related to women's health. With the improvement of the evaluation system of vaginal microecology, vaginal microecology-related testing services have been widely carried out in hospitals and testing institutions. The current detection methods include morphological observation of vaginal secretions (microscopic examination) and functional index detection. Microscopic examination included the density and diversity of vaginal flora, pathogenic microorganisms (fungus and trichomonas), Nugent score, white blood cell count, etc. Functional indicators include detection of hydrogen per...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/28C12Q1/44C12Q1/34C12Q1/37C12Q1/56C12M1/34C12M1/40G01N21/80
Inventor 姜南任红军黄明辉赵雷江淑芳
Owner GENETEL PHARMA SHENZHEN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap