Exosome purification method and all-in-one machine device thereof

A purification method and exosome technology, applied in the biological field, can solve the problems of high cost, long duration, complicated purification process, etc., and achieve the effects of short time, reducing damage and solving insufficient production capacity.

Pending Publication Date: 2022-04-12
宁波甬恒瑶瑶智能科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide an exosome purification method to solve the problems of cumbersome purification process, long duration and high cost of conventional exosome purification methods currently on the market

Method used

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  • Exosome purification method and all-in-one machine device thereof
  • Exosome purification method and all-in-one machine device thereof
  • Exosome purification method and all-in-one machine device thereof

Examples

Experimental program
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Embodiment 1

[0033]This embodiment provides an integrated equipment for exosome purification, which specifically includes a tangential flow ultrafiltration system 1, a fast protein liquid chromatography system 2, a nanoparticle tracer analysis system 3, and a driving Peristaltic pump; the peristaltic pump is used to drive the flow of liquid inside the equipment; by adding the liquid to be extracted containing exosomes to the equipment, the liquid to be extracted flows through the tangential flow ultrafiltration system in sequence 1. Fast protein liquid chromatography system 2. The nanoparticle tracer analysis system 3 completes the purification of exosomes;

[0034] The tangential flow ultrafiltration system 1 includes a first-stage tangential-flow ultrafiltration device 11 and a second-stage tangential-flow ultrafiltration device 12 connected to each other, and the first-stage tangential-flow ultrafiltration device 11 is provided with First stage inlet 111, first stage outlet 112, describ...

Embodiment 2

[0039] This embodiment provides a method for purifying exosomes, comprising the following steps:

[0040] S1 Secondary tangential ultrafiltration: pass the extract containing exosomes into the first-stage tangential flow ultrafiltration device, and pass through the first-stage ultrafiltration membrane to recover the target liquid after ultrafiltration to obtain permeate , to trap impurities; then the permeate will continue to flow through the second-stage tangential flow ultrafiltration device, and the ultrafiltration membrane pore size of the second-stage tangential flow ultrafiltration device is smaller than that of the exosomes , intercepting it, recovering the filtrate to obtain the enriched solution;

[0041] The pore diameter of the ultrafiltration membrane in the first-stage tangential flow ultrafiltration device is larger than the pore diameter of the ultrafiltration membrane in the second-stage tangential flow ultrafiltration device, and the ultrafiltration membrane i...

Embodiment 3

[0048] This embodiment provides a method for purifying exosomes, comprising the following steps:

[0049] S1 Secondary tangential ultrafiltration: pass the extract containing exosomes into the first-stage tangential flow ultrafiltration device, and pass through the first-stage ultrafiltration membrane to recover the target liquid after ultrafiltration to obtain permeate , to trap impurities; then the permeate will continue to flow through the second-stage tangential flow ultrafiltration device, and the ultrafiltration membrane pore size of the second-stage tangential flow ultrafiltration device is smaller than that of the exosomes , intercepting it, recovering the filtrate to obtain the enriched solution;

[0050] The pore diameter of the ultrafiltration membrane in the first-stage tangential flow ultrafiltration device is larger than the pore diameter of the ultrafiltration membrane in the second-stage tangential flow ultrafiltration device, and the ultrafiltration membrane i...

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Abstract

The invention provides an exosome purification integrated method which comprises the following steps: S1, secondary two-stage tangential ultrafiltration, S2, pretreatment of a to-be-extracted solution, S3, particle size and concentration detection of exosomes and S4, freeze-drying, and the invention further provides an exosome purification integrated device. The exosome can be well purified through the method, the method has high practical value, and the problems that a conventional exosome purification method in the market at present is tedious in purification process, long in time and high in cost are solved.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to an exosome purification method and an all-in-one device. Background technique [0002] In 2013, the Nobel Prize in Physiology or Medicine was awarded to three scientists who studied the regulatory mechanism of cell vesicle transport. After that, exosome research gradually became a scientific research hotspot. Exosomes are small vesicles with a diameter of about 30-150nm secreted by living cells. They have a typical lipid bilayer structure and are widely found in cell culture supernatant, blood, saliva, urine, and semen. , amniotic fluid and other biological fluids. It carries a variety of important information such as proteins, lipids, and RNA, and plays an important role in the transmission of substances and information between cells. It can regulate cell metabolism, cell apoptosis, and immune regulation. It can be used as a medical beauty, regeneration Medicine, disease d...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/12C12M1/00C12N5/00
CPCB01D61/146B01D2311/06B01D2315/10B01D2311/25B01D2317/025B01D2317/06B01D2311/2697C07K1/34C07K1/16C07K1/36
Inventor 何加铭李思徐文利王硕
Owner 宁波甬恒瑶瑶智能科技有限公司
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