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Method for extracting intact chloroplast from ginkgo leaves

A technology of Ginkgo biloba leaves and an extraction method, which is applied in the field of biochemistry and can solve the problems of low yield, low chloroplast integrity, difficulty in chloroplast extraction and the like

Inactive Publication Date: 2011-04-20
NANJING NORMAL UNIVERSITY
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  • Abstract
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  • Claims
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Problems solved by technology

[0010] The object of the present invention is to provide a high-yield method for extracting the complete chloroplasts of medicinal woody plants rich in secondary metabolites, so as to overcome the difficulty in extracting chloroplasts of such plants, the low yield and the low integrity of chloroplasts defects, thereby establishing a method for extracting complete chloroplasts of medicinal woody plants with a yield higher than 80%

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  • Method for extracting intact chloroplast from ginkgo leaves
  • Method for extracting intact chloroplast from ginkgo leaves
  • Method for extracting intact chloroplast from ginkgo leaves

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Embodiment Construction

[0031] Terms in the present invention are explained as follows:

[0032] Percoll: Percoll is a suspension of silica gel particles treated with polyvinyl pyrrolidone (PVP), which is non-toxic and irritating to cells. The silica gel particles of Percoll suspension are of different sizes. After high-speed centrifugation, a continuous density gradient can be formed to separate and purify cells with different specific gravity. Percoll purchased from Sigma Company is used in the present invention.

[0033] 2× Chloroplast Suspension: refers to double the concentration of the above chloroplast suspension, that is, 40mM Tricine-KOH, 0.6M Sorbitol, 10mM MgCl 2. 6H 2 O, 5mM EDTA-Na 2 .

[0034] 1. Materials and methods

[0035] 1.1 Materials

[0036] Mature leaves of Ginkgo biloba were collected from Xianlin Campus, Nanjing Normal University, Jiangsu Province.

[0037] Extraction Solutions and Media

[0038] Chloroplast Crude Extract: 20mM Tricine-KOH (pH 8.4), 0.45 M Sorbitol, ...

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Abstract

The invention relates to a technology for extracting intact chloroplast from high-yield officinal woody plant ginkgo rich in secondary metabolites, belonging to the biochemistry field. The method of the invention comprises the following steps of: preparing coarse chloroplast granules from ginkgo leaves in a special growth period, and then carrying out gradient centrifugation to Percoll to acquire chloroplast through special centrifugal force. The invention can effectively improve the purity and the integrity of the extracted chloroplast, the ginkgo chloroplast obtained by adopting the method reaches the integrity rate of above 80 percent, and the extracted chloroplast does not contain other impurities. The chloroplast granules are suitable for fundamental researches on cell biology, biochemistry, molecular biology, and the like.

Description

technical field [0001] The invention relates to the extraction and separation technology of the complete chloroplast of the leaves of the woody plant Ginkgo biloba, belonging to the technical field of biochemistry. Background technique [0002] ginkgo( Ginkgo biloba L . ), is the oldest relict plant in the existing seed plants, and is also a unique and precious tree species that originated in my country. my country is the hometown of ginkgo, and the existing resources account for more than 70% of the world's ginkgo resources. The medicinal value of ginkgo leaves has been highly valued at home and abroad in recent years. The active substances extracted from ginkgo leaves have good effects on softening blood vessels, preventing arteriosclerosis, clearing blood fat garbage, inhibiting platelet aggregation, and preventing cerebral thrombosis. It has powerful functions such as preventing middle-aged and elderly dementia, changing retinal distance, enhancing immunity, and delayi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/00C12N15/29
Inventor 陈国祥魏晓东
Owner NANJING NORMAL UNIVERSITY
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