Method for extracting and detecting yeast beta-glucan in protein powder

Abstract

The invention discloses an extraction and detection method of yeast beta-glucan in protein powder, and the extraction method comprises the following steps: adding a pretreatment liquid into a sample, uniformly mixing, and carrying out water bath treatment to obtain a treatment liquid; the detection method specifically comprises the following steps: extracting the yeast beta-glucan in a sample by using the extraction method, and determining the yeast beta-glucan by using a sulfuric acid-phenol method. Compared with the prior art, the yeast beta-glucan can be extracted, separated and purified from the protein powder, the extraction method is simple and accurate, and the detection cost is low.

Description

technical field [0001] The invention belongs to the technical field of food detection, and in particular relates to an extraction and detection method of yeast beta-glucan in protein powder. Background technique [0002] Yeast β-glucan is a national new food raw material. It is a polysaccharide that exists in the yeast cell wall and has the ability to enhance immunity. It also has the effects of improving blood lipids, anti-radiation, and improving intestinal function. [0003] Due to the low amount of yeast β-glucan added in food and the many and complex matrices, the detection method of yeast β-glucan raw materials is not suitable for the detection of yeast β-glucan in food, such as "QB / T 4572-2021 Yeast beta-glucan and USP methods. The current detection methods for yeast β-glucan in food include "QB / T4572-2021 Yeast β-glucan Appendix A" and "National Food Safety Standard Determination of Yeast β-glucan in Food-Draft for Comment" , and the contents of the two methods are...

AI Technical Summary

Problems solved by technology

Dairy foods can be treated with this method to separate yeast β-glucan with relatively high purity, and the amount of residue is also very small, which is helpful for subsequent alkali treatment and quantitative operations. Yeast β-glucan protein powder is The amount of residue obtained by the method treatment is very large, which affects the subsequent alkali treatment and quantitative operation, indicating that the method has insufficient ability to remove impurities from yeast β-glucan protein powder

Therefore, yeast beta-glucan protein powder is not well suited for this method

[0005] 2. The precision of the method. This method does not have a strict constant volume operation, which will cause certain losses during the extraction and separation of yeast β-glucan. The dilution operation magnifies the detection error, so the precision of the method can only be ≤15%

[0006] 3. The operability of the method. The method needs to introduce different blank samples and yeast β-glucan reference substances according to different varieties each time to correct the test results. The introduction of blank samples will increase the detection amount, and the different formulations must be retained Blank sample, and there are special requirements for the yeast β-glucan reference substance, which must be water-insoluble yeast β-glucan, which is difficult to achieve for daily detection and external detection; the method has many steps and requires high human operation ability , the entire detection process takes 2-3 days, and the detection efficiency is not high; and there are many factors affecting the enzymatic hydrolysis process, it is difficult to control the detection process

[0007] 4. The cost of the method, the enzymes required by the method are expensive, and the cost of testing large quantities of samples is high

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