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Anti-inflammatory amniotic membrane patch and preparation method thereof

A technology of amniotic membrane and adipose tissue, applied in anti-inflammatory agents, pharmaceutical formulas, medical preparations with non-active ingredients, etc., can solve the problems of slow wound recovery and achieve the effects of preventing scar formation, ensuring persistence and improving activity

Active Publication Date: 2022-04-08
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the present invention provides an anti-inflammatory amniotic membrane application and a preparation method thereof, which can effectively solve the problem of slow wound recovery in the existing application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] An anti-inflammatory amniotic membrane application, its preparation method comprises the following steps:

[0025] (1) Prepare decellularized amnion; specifically: peel off the washed placenta to obtain amnion, purify the amnion with peracetic acid / sodium chloride mixed solution, and remove the amount of peracetic acid in the peracetic acid / sodium chloride mixed solution The volume concentration is 0.1%, the mass concentration of sodium chloride is 0.8mol / l, and then the amniotic membrane is inactivated and washed to obtain the inactivated amniotic membrane. Add trypsin solution to the inactivated amniotic membrane and digest at 35°C for 150min After washing, the mass concentration of trypsin solution is 0.25%, and the consumption of trypsin solution is 0.17ml / cm 2 , wash the washed inactivated amnion with detergent solution (Triton X-100) at 35°C for 5 h, then immerse the washed inactivated amnion in peracetic acid with a volume concentration of 0.1% for 5 h to sterili...

Embodiment 2

[0031] An anti-inflammatory amniotic membrane application, its preparation method comprises the following steps:

[0032] (1) Prepare decellularized amnion; specifically: peel off the washed placenta to obtain amnion, purify the amnion with peracetic acid / sodium chloride mixed solution, and remove the amount of peracetic acid in the peracetic acid / sodium chloride mixed solution The volume concentration is 0.1%, the mass concentration of sodium chloride is 1.2mol / l, and then the amniotic membrane is inactivated and washed to obtain the inactivated amniotic membrane, which is added to the inactivated amniotic membrane in trypsin solution and digested at 39°C for 220min After washing, the mass concentration of trypsin solution is 0.4%, and the consumption of trypsin solution is 0.25ml / cm 2 , wash the washed inactivated amnion with detergent solution (Triton X-100) at 40°C for 6 hours, then soak the washed inactivated amnion in peracetic acid with a volume concentration of 0.1% fo...

Embodiment 3

[0038] An anti-inflammatory amniotic membrane application, its preparation method comprises the following steps:

[0039] (1) Prepare decellularized amnion; specifically: peel off the washed placenta to obtain amnion, purify the amnion with peracetic acid / sodium chloride mixed solution, and remove the amount of peracetic acid in the peracetic acid / sodium chloride mixed solution The volume concentration is 0.1%, the mass concentration of sodium chloride is 1mol / l, and then the amniotic membrane is inactivated and washed to obtain the inactivated amniotic membrane, which is added to the inactivated amniotic membrane in trypsin solution and digested at 37°C for 200 minutes Wash, the mass concentration of trypsin solution is 0.3%, the consumption of trypsin solution is 0.2ml / cm 2 , wash the washed inactivated amnion with detergent solution (Triton X-100) at 37°C for 5 hours, then immerse the washed inactivated amnion in peracetic acid with a volume concentration of 0.1% for 5 hours ...

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Abstract

The invention discloses an anti-inflammatory amniotic membrane patch and a preparation method thereof. The preparation method comprises the following steps: preparing a decellularized amniotic membrane; the preparation method comprises the following steps: respectively preparing adipose-derived stem cells and an adipose tissue vascular matrix, then adding the adipose-derived stem cells and oil into the adipose tissue vascular matrix, mixing until flocculate appears, centrifuging, and removing lower-layer swelling liquid and upper-layer grease to prepare a gel-like matrix; and smearing the gel-like matrix on the rough surface side of the decellularized amniotic membrane to obtain the gel-like amniotic membrane. The anti-inflammatory amniotic membrane patch can effectively solve the problem that wound recovery is slow in an existing patch.

Description

technical field [0001] The invention relates to the technical field of application, in particular to an anti-inflammatory amniotic membrane application and a preparation method thereof. Background technique [0002] Wound healing refers to a series of pathophysiological processes in which local tissues are repaired through regeneration, repair, and reconstruction after tissue loss due to the action of injury factors. In essence, it is an inherent defensive adaptive response of the body to tissue cell damage caused by various harmful factors. This regenerative repair is manifested in the restoration of lost tissue structure, and can also restore its function to varying degrees. [0003] However, the repair of lost tissue cells can be the "complete recovery" of the original tissue cells, which is called "regeneration", or it can be replaced by non-specific connective tissue proliferation, which is called "incomplete recovery". It is called "repair", but the process of these ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/70A61K47/46A61K35/35A61K35/28A61P17/02A61P29/00C12N5/0775
CPCY02A50/30
Inventor 高雪华江蕊利侯爽梁琳华赵乐乐王威何钰洁樊慧海泉
Owner CHENGDU QINGKE BIOTECH
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