Application of eriocitrin in preparation of LPS-induced sepsis acute lung injury protection medicine

An acute lung injury and eriocitrin technology, applied in the field of biomedicine, can solve problems that have not been reported at home and abroad, and achieve good curative effect, reduced anti-inflammatory effect, and promising effects

Active Publication Date: 2022-03-29
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although multiple pharmacological activities of eriocitrin have been reported in the literature, there are no domestic and foreign reports on its preventive effect on acute lung injury caused by sepsis

Method used

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  • Application of eriocitrin in preparation of LPS-induced sepsis acute lung injury protection medicine
  • Application of eriocitrin in preparation of LPS-induced sepsis acute lung injury protection medicine
  • Application of eriocitrin in preparation of LPS-induced sepsis acute lung injury protection medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Detection of Inflammatory Indexes in Mouse Pulmonary Tissue

[0042] The lung tissue was collected in formaldehyde tissue fixative, embedded in paraffin, sectioned, and stained with HE. The result is as figure 1 As shown in A-B, compared with the control group, the LPS group can cause a large amount of inflammatory substances and red blood cells in the alveoli to exudate, the alveolar structure is destroyed, and the injury score is higher, while the eriocitrin administration group can significantly improve the pathological damage of lung tissue ; In order to reflect the pulmonary edema situation of each group of mice, the wet and dry weight of the lung tissue was measured, such as figure 1 C, Compared with the control group, the ratio of wet / dry weight in the LPS group was significantly increased, while the ratio of wet / dry weight in the eriocitrin administration group was significantly reduced; Western blot was used to evaluate the protein expression levels ...

Embodiment 2

[0044] Example 2 Detection of Oxidative Stress in Mouse Lung Tissue

[0045] GSH-PX, SOD activity determination and MDA expression level determination were carried out on mouse lung tissue with biochemical kits, and active oxygen immunofluorescence staining was performed on mouse lung tissue. The results were as follows: figure 2 As shown in A-D, compared with the control group, the activities of GSH-PX and SOD in the lung tissue of the mice in the LPS group were significantly reduced, and the expression level of MDA and the fluorescence intensity of ROS were significantly increased. SOD activity, MDA expression and ROS fluorescence intensity were inhibited, and the effect of high dose group and lower dose group was more obvious (*p<0.05vs.control group, #p<0.05vs.LPS group).

[0046] In acute lung injury, it is often accompanied by excessive activation of oxidative stress response, which causes the imbalance of oxidation / antioxidation in lung tissue, and the generation of a ...

Embodiment 3

[0047] Example 3 Detection of Inflammatory Effects on LPSylated Thp1 Cells

[0048] Western blot was used to detect the protein expression of MPO, p-P65, and P65 in Thp1 cells, and ELISA was used to measure the expression of pro-inflammatory factors in Thp1 cell culture supernatant, such as image 3 As shown in A-D, compared with the control group, the expression levels of MPO, p-P65 / P65 protein, TNF-α, IL-6, and IL-1β in the LPS group were significantly increased, while the eriocitrin administration group could be significantly down-regulated The expression of MPO, p-P65 / P65 protein, and pro-inflammatory factors, and the lung protection effect of the high-dose group and the lower-dose group was more obvious (*p<0.05vs.control group, #p<0.05vs.LPS group).

[0049] In the early stage of acute lung injury, monocytes are activated and further polarized into M1 macrophages, which release a large number of pro-inflammatory factors and aggravate inflammation. This example shows tha...

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Abstract

The invention discloses application of eriocitrin in preparation of LPS (lipopolysaccharide)-induced acute lung injury sepsis protection medicines, belongs to the technical field of biological medicines, provides application of eriocitrin in preparation of medicines for preventing and / or treating acute lung injury sepsis, and performs experimental research by constructing a model, and provides a new application of eriocitrin in preparation of medicines for preventing and / or treating acute lung injury sepsis. The result shows that eriocitrin has a good protection effect on the LPS-induced sepsis acute lung injury, has small side effects, and can be used for preparing the protective medicine for the LPS-induced sepsis acute lung injury. The invention provides a novel application of eriocitrin in preparation of a sepsis lung injury protective medicine, and the eriocitrin has obvious effects in the aspects of improving tissue injury, playing an anti-inflammatory effect and reducing oxidative stress and has small side effects.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of eriocitrin in the preparation of LPS-induced sepsis acute lung injury protection medicine. Background technique [0002] Sepsis is a systemic inflammatory response syndrome caused by bacteria and other pathogenic microorganisms invading the body through various channels. It is one of the common critical illnesses in clinical work. Accompanied by multiple organ dysfunction, or even failure, the cost of treatment is high, and the consumption of medical resources is large, which brings a huge burden to society and human health. Sepsis can be caused by infection at any site and often occurs in severely ill patients. The clinical manifestations are fever, tachycardia, shortness of breath, and peripheral blood leukocytosis. Its pathogenic mechanism is complex and diverse, including systemic inflammatory response, immune dysfunction, abnormal coagulation function...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61K36/752A61P11/00A61P31/04A61P29/00A61P39/06
CPCA61K31/7048A61K36/752A61P11/00A61P31/04A61P29/00A61P39/06
Inventor 耿庆李宁李东航王博吴小静
Owner WUHAN UNIV
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