Method for removing endotoxin in foot-and-mouth disease antigen liquid

Abstract

The invention relates to a method for removing endotoxin in a foot-and-mouth disease antigen, which adopts strong anion exchange chromatography for separation and comprises the following steps: (1) balancing: balancing a chromatographic column by using equilibrium liquid; (2) adjustment: calculating the loading volume according to the endotoxin content of the foot-and-mouth disease antigen liquid, and if the loading total endotoxin content is lower than the maximum loading capacity of a chromatographic column, taking the antigen liquid with the calculated volume, adjusting the conductivity to 18-22mS / cm, and then loading; (3) collection: after the antigen liquid is loaded, when an ultraviolet absorption curve rises, starting to collect a chromatography permeate, after the loading is finished, continuously feeding the equilibrium liquid into the column, and simultaneously continuously collecting the chromatography permeate until the ultraviolet absorption curve is reduced to be gentle; and (4) regeneration: regenerating the chromatographic column by using the regeneration liquid. The method is simple to operate, suitable for large-scale production and application, high in antigen recovery rate and high in endotoxin removal rate.

Description

technical field [0001] The invention relates to the field of veterinary medicine, in particular to a method for removing endotoxin in a foot-and-mouth disease antigen solution. Background technique [0002] Foot-and-mouth disease is an acute, febrile, highly contagious infectious disease common to pigs, cattle, sheep and other domestic and wild cloven-hoofed animals, with more than 70 susceptible animals. The clinical feature is a vesicular eruption on the oral mucosa, hooves, and breast skin. The disease has many transmission routes and fast speed, and has repeatedly broken out in the world, causing huge political and economic losses. In view of this, the World Organization for Animal Health (OIE) ranks it as the first class A infectious disease. At present, FMD is prevalent in two-thirds of OIE member countries, threatening the safety of livestock and the trade of livestock products in non-FMD countries and regions. [0003] Foot-and-mouth disease virus belongs to the f...

AI Technical Summary

Problems solved by technology

However, buffer solution is continuously added during the diafiltration process to achieve the purpose of further rinsing and purifying the antigen solution, which will lead to a certain degree of degradation of the antigen. The antigen loss rate in the diafiltration process can generally reach more than 20%, and due to the limitation of the pore size, this This method has a certain limit for the removal of endotoxin, and the removal rate of endotoxin is generally up to 75%.

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