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Preparation method of magnetic graphene oxide/multilayer nanocomposite immobilized tannase

A nanocomposite material, iron oxide nanotechnology, applied in the direction of immobilized enzymes, biochemical equipment and methods, immobilized on or in inorganic carriers, etc., can solve the problems of enzyme loss of activity, reduction, etc., to improve pH value and The effect of heat resistance

Active Publication Date: 2022-03-22
ZHEJIANG FORESTRY UNIVERSITY
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Problems solved by technology

However, the separation of GO from aqueous solutions requires additional filtration or high-speed centrifugation
In this method, heavy mechanical stress can lead to significant enzyme loss and reduced activity

Method used

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  • Preparation method of magnetic graphene oxide/multilayer nanocomposite immobilized tannase
  • Preparation method of magnetic graphene oxide/multilayer nanocomposite immobilized tannase
  • Preparation method of magnetic graphene oxide/multilayer nanocomposite immobilized tannase

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Embodiment Construction

[0048] The present invention will be further described below in conjunction with example.

[0049] Materials and reagents are as follows:

[0050] Ethylene glycol (EG), ferric chloride hexahydrate (FeCl 3 ·6H 2 O), sodium acetate (NaAc), sodium citrate (Na 3 Cit), polyethylene glycol (PEG 2000), sodium hyaluronate (HA), methyl gallate, rhodanine, potassium hydroxide and gallic acid were all purchased from Adamas Reagents. GO nanosheets were obtained from Nanjing Pioneer Nanotechnology Co., Ltd. Tannase was purchased from Solelab Biotech Co., Ltd. Life Sciences Co., Ltd. Branched polyethyleneimide (PEI, average molecular weight 10kDa), N-hydroxysuccinimide (NHS) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) Purchased from Sigma Aldrizzi. Methanol was purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd. All chemicals were used as received without further purification.

[0051] A method for preparing magnetic graphene oxide / multilayer nanoco...

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Abstract

The invention belongs to the technical field of immobilized enzymes, and discloses a magnetic graphene oxide / multilayer nano composite material immobilized tannase and a preparation method thereof. The magnetic graphene oxide / multi-layer polymer nano composite material is prepared and used as a carrier to be covalently connected with tannase, good stability and reusability are obtained, and the magnetic graphene oxide / multi-layer polymer nano composite material can be applied to degradation of torreya grandis extract tannin. Benefited from the flexible arm and numerous anchoring points of the multi-layer polymer, the functions of genipin activation, natural biological crosslinking and covalent immobilization and unique magnetic separation characteristics, the prepared nano biological composite material has stable enzymatic activity. The catalytic efficiency, the thermal stability, the reusability, the storability and the practical application of the magnetic graphene oxide / multilayer polymer nano composite material prove the stability, the adaptability and the practicability of the immobilized tannase.

Description

technical field [0001] The invention relates to the technical field of immobilized enzymes, in particular to a magnetic graphene oxide / multilayer nanocomposite immobilized tannase and a preparation method thereof. Background technique [0002] The abundance of tannins in plant tissues and their potential for biotransformation into sustainable chemicals such as gallic acid has become a research hotspot in biotechnology to maximize the utilization of natural compounds. Tannins protect plants from microbes. Due to the high content of tannins, some teas, fruit juices, beverages and dried fruits have astringent, cloudy and bitter tastes, which can lead to a significant reduction in the human body's sense of taste. In addition, tannin is one of the main components of tanning industry effluents, posing a potential threat to human health, animals, and the environment. Therefore, the treatment of tannins is consistent with the health and treatment goals of the tanning industry. Ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/089C12N9/18C08J3/24C08L5/08C08L71/02C08K3/22C08K3/04
CPCC12N11/14C12N11/089C12N9/18C12Y301/0102C08J3/24C08J2305/08C08J2471/02C08J2371/02C08J2405/08C08K2003/2265C08K3/042
Inventor 郇伟伟吴家胜宋丽丽李洁张宜明韩旖旎郭明高飞刘彬刘鹏辉李路明李京蓉
Owner ZHEJIANG FORESTRY UNIVERSITY
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