Preparation method of magnetic graphene oxide/multilayer nanocomposite immobilized tannase
A nanocomposite material, iron oxide nanotechnology, applied in the direction of immobilized enzymes, biochemical equipment and methods, immobilized on or in inorganic carriers, etc., can solve the problems of enzyme loss of activity, reduction, etc., to improve pH value and The effect of heat resistance
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[0048] The present invention will be further described below in conjunction with example.
[0049] Materials and reagents are as follows:
[0050] Ethylene glycol (EG), ferric chloride hexahydrate (FeCl 3 ·6H 2 O), sodium acetate (NaAc), sodium citrate (Na 3 Cit), polyethylene glycol (PEG 2000), sodium hyaluronate (HA), methyl gallate, rhodanine, potassium hydroxide and gallic acid were all purchased from Adamas Reagents. GO nanosheets were obtained from Nanjing Pioneer Nanotechnology Co., Ltd. Tannase was purchased from Solelab Biotech Co., Ltd. Life Sciences Co., Ltd. Branched polyethyleneimide (PEI, average molecular weight 10kDa), N-hydroxysuccinimide (NHS) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) Purchased from Sigma Aldrizzi. Methanol was purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd. All chemicals were used as received without further purification.
[0051] A method for preparing magnetic graphene oxide / multilayer nanoco...
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