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Tissue culture method of North China scabiosa

A technology of tissue culture and blue potted flower, applied in the field of plant biology, can solve the problems of the shortage of natural resources of blue potted flower in North China, the vulnerability to man-made or natural hazards, and the inability to guarantee stable genetics, etc. The effect of implementation

Active Publication Date: 2022-03-22
INNER MONGOLIA AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, people's requirements for life have been continuously improved, and the demand for wild medicinal materials including North China blue potted flowers has gradually increased, but the market possession has decreased year by year, and North China blue potted plants grow in the wild and are vulnerable to man-made or natural hazards In addition, the propagation method of North China blue potted flowers is seed propagation, and the offspring are easy to mutate, and stable inheritance cannot be guaranteed. Therefore, the natural resources of North China blue potted flowers are in short supply, and the market is in short supply. The traditional seed propagation method of North China blue potted flowers is slow , The reproduction volume is small, it cannot be reproduced on a large scale, and it cannot meet the market demand

Method used

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  • Tissue culture method of North China scabiosa
  • Tissue culture method of North China scabiosa
  • Tissue culture method of North China scabiosa

Examples

Experimental program
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Effect test

Embodiment 1

[0047] The invention provides a method for tissue culture of North China blue potted flower, comprising the following steps:

[0048]S1. Disinfection: Select the complete leaves on the upper part of the vigorously growing plants without diseases and insect pests, after washing, move to the ultra-clean workbench, first sterilize with 75% alcohol for 28 seconds, and then use sterile water for 8 minutes to wash away the residue of the disinfectant. filter paper to absorb moisture;

[0049] S2, callus induction: place the leaves sterilized in step 1 in a petri dish, cut off the edges of the leaves, cut the remaining leaves into square explants, and cut 1-3 small openings on the square explants , or scratch the leaf surface, inoculate the leaves face up into callus induction medium to induce callus, and the culture time is 38-43 days. The callus medium is 30.00g sucrose + 6.50g agar +MS+1.50-2.50mg / L TDZ;

[0050] S3. Inducing callus differentiation: cut the callus induced in ste...

Embodiment 2

[0061] A method for tissue culture of North China blue potted flower, comprising the following steps:

[0062] S1. Disinfection: Select the complete leaves on the upper part of the vigorously growing plant without diseases and insect pests, after washing, move to the ultra-clean workbench, first disinfect with 75% alcohol for 32 seconds, then soak in 2% sodium hypochlorite for 9 minutes, and wash the disinfectant with sterile water Residue, blot dry with sterile filter paper;

[0063] S2, callus induction: place the leaves sterilized in step 1 in a petri dish, cut off the edges of the leaves, cut the remaining leaves into square explants, and cut 1-3 small openings on the square explants , or scratch the leaf surface, inoculate the leaves face up into callus induction medium to induce callus, and the culture time is 38-43 days. The callus medium is 30.00g sucrose + 6.50g agar +MS+1.50-2.50mg / L TDZ;

[0064] S3. Inducing callus differentiation: cut the callus induced in step ...

Embodiment 3

[0075] A method for tissue culture of North China blue potted flower, comprising the following steps:

[0076] Step 1. Disinfection: Select the complete leaves on the upper part of the vigorously growing plant without diseases and insect pests, after washing, move to the ultra-clean workbench, first disinfect with 75% alcohol for 30 seconds, then soak in 2% sodium hypochlorite for 9 minutes, wash and disinfect with sterile water For drug residues, dry the water with sterile filter paper;

[0077] Step 2, callus induction: place the leaves sterilized in step 1 in a petri dish, cut off the edges of the leaves, cut the remaining leaves into square explants, and cut 1-3 on the square explants Make a small cut, or scratch the leaf surface, inoculate the leaf face up into callus induction medium to induce callus, and the culture time is 38-43 days. The callus medium is 30.00g sucrose + 6.50g Agar+MS+1.50-2.50mg / L TDZ;

[0078] Step 3. Inducing callus differentiation: cut the callu...

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Abstract

The invention discloses a tissue culture method of northern China potted flowers, which comprises the following steps: step 1, disinfection: firstly using 75% alcohol and then using 2% sodium hypochlorite for disinfection; step 2, callus induction: cutting the disinfected leaves into squares, and inoculating the squares into a callus induction culture medium to induce callus, and step 3, callus differentiation induction: cutting the callus into cube small blocks, inoculating the cube small blocks into a callus induction differentiation culture medium to differentiate adventitious buds, inoculating the adventitious buds of 6-8cm into a root culture medium, and culturing the adventitious buds in the root culture medium. Adventitious buds are promoted to root to form regenerated seedlings.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a method for tissue culture of North China blue potted flowers. Background technique [0002] North China blue potted flower (scientific name: Scabiosa tschiliensis Grun.) is a Diphtheraceae family. Its flower shape is beautiful and colorful. It has the characteristics of light-loving, cold-resistant, drought-resistant and barren-resistant. It is a perennial herb flower and an important wild medicinal plant. North China blue potted flower is rich in flavonoids, Saponins and polysaccharides have anti-inflammatory, antipyretic, anti-oxidant, sedative and immune-enhancing effects, and have good therapeutic effects on the treatment of hepatitis jaundice, lung heat cough, sore throat, high blood pressure, and low immune function. High medicinal value and economic value. [0003] In recent years, people's requirements for life have been continuously improved, and the de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G24/28A01G24/15A01G24/12A01G22/60
CPCA01H4/002A01H4/008A01G24/28A01G24/15A01G24/12A01G22/60Y02P60/40
Inventor 白瑞琴尹晶晶刘思泱彭伯阳吕艳芳范旭东陈佳蕊马怀林陈羿如
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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