Prevention and treatment method for anthracnose of amomum tsao-ko
An anthracnose, grass and fruit technology, which is applied in the field of plant protection, can solve the problems of lack of cultivation and disease prevention and control measures and management methods, and achieves the effects of reducing the risk of drug resistance, significant control effect, and reducing the amount and frequency of use of pesticides
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[0015] The present invention will be further described below in conjunction with embodiment.
[0016] test 1
[0017] Bacteriostasis test of pesticides on the fungus of anthracnose
[0018] 1. Identification of pathogenic bacteria
[0019] 1.1 Potato dextrose agar (potato dextroseagar, PDA) medium: glucose 20g, potatoes 200g, agar 20g, distilled water 1000mL.
[0020] 1.2 Isolation and purification of pathogenic bacteria Cut out 5mm×5mm tissue at the junction of diseased anthracnose, disinfect in 70% alcohol for 5s, disinfect with 0.1% mercuric chloride for 30s, rinse with sterile water for 3 times, place in On the PDA plate, after culturing in an incubator at 25±2°C for 5 days, the hyphae at the edge of the colony were picked for isolation. The obtained strains were further purified and cultured and stored in a refrigerator at 4°C until use.
[0021] 1.3 Inoculation of pathogenic bacteria Anthracnose bacteria cultured in PDA for 7 days, and the edge of the anthracnose bac...
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