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Method for inhibiting harmful algae by applying perylenequinone compound

A compound, perylenequinone technology, applied in the field of water pollutant treatment, can solve the problem of lack of economical and effective control of cyanobacteria blooms, and achieve the effect of good algae inhibition and low cost

Active Publication Date: 2022-03-11
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the current lack of economical and effective methods for controlling harmful algae such as cyanobacteria blooms, and provides an application of perylenequinone compounds in inhibiting the growth of harmful algae

Method used

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  • Method for inhibiting harmful algae by applying perylenequinone compound
  • Method for inhibiting harmful algae by applying perylenequinone compound
  • Method for inhibiting harmful algae by applying perylenequinone compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Detection method: Perylene quinone compounds are effective against Microcystis aeruginosa (FACHB-905), Anabaena (FACHB-82), Astringentia floridae (FACHB-1040), Oscillator algae (FACHB-528), A. Algae (FACHB-920) survival rate with hemacytometer. The counting of the counting board uses the Tangmai counting method to divide the large square into 25 middle grids, and each middle grid is divided into 16 small grids, that is, 25×16 type. The calculation formula is the number of algae cells / L=N×5× 10×dilution factor, N is: the total number of algae cells in the five squares. [Example 1] The inhibitory effect of cercosporin (CP) on Microcystis aeruginosa (FACHB-905)

[0044] Adjust the density of algal cells to 1.6×10 7 cells / mL cell suspension, inoculated in 6-well plate, 4 mL per well; 20 μM cercosporin (CP) was added to the experimental group, and three replicate wells were set up in the experimental group and control wells respectively. Place the 6-well plate at room tem...

Embodiment 2

[0048] [Example 2] The inhibitory effect of cercosporin (CP) on Anabaena (FACHB-82)

[0049] Adjust the density of algal cells to 1.6×10 7 cells / mL cell suspension, inoculated in 6-well plate, 4 mL per well; 20 μM CP was added to the experimental group, and three replicate wells were set up in the experimental group and control wells respectively. Place the 6-well plate at room temperature and react under 23W white light. The cells at 12h, 24h, and 36h were counted respectively, and compared with the cells in the control group, the survival rate was determined. The formula for calculating the survival rate is as formula (1) in Example 1.

[0050] The results showed that after 36 hours, the cell survival rate of 20μM CP inhibiting the effect of Anabaena was 23%.

Embodiment 3

[0051] [Example 3] Inhibitory effect of cercosporin (CP) on A. water blooms (FACHB-1040)

[0052] Adjust the density of algal cells to 1.6×10 7 cells / mL cell suspension, inoculated in 6-well plate, 4 mL per well; 20 μM CP was added to the experimental group, and three replicate wells were set up in the experimental group and control wells respectively. Place the 6-well plate at room temperature and react under 23W white light. The cells at 12h, 24h, and 36h were counted respectively, and compared with the cells in the control group, the survival rate was determined. The formula for calculating the survival rate is as formula (1) in Example 1.

[0053] The results showed that: after 36h, the cell survival rate of 20μM CP inhibiting the effect of A. water bloom was 21%.

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Abstract

The invention discloses a method for inhibiting harmful algae by applying perylenequinones compounds, and belongs to the technical field of environmental application. The perylenequinone compound provided by the invention can obviously inhibit the growth of harmful algae in the absence of illumination or visible illumination radiation, so that the cell surface is unsmooth and the cell morphology is incomplete; cell membranes, cell walls and thylakoid structures can be destroyed; degrading fatty acid in a cyanobacteria cell membrane; the content of chlorophyll a is degraded; growth of blue-green algae in the actual environment of the Taihu Lake is According to the method, the perylenequinone compound is used as the algistat for broad-spectrum inhibition of blue-green algae, and the application prospect is very wide.

Description

technical field [0001] The invention specifically relates to a method for inhibiting harmful algae by using a perylenequinone compound, and belongs to the technical field of water pollutant treatment. Background technique [0002] With the development of urbanization in my country, a large amount of nitrogen, phosphorus and other biologically required nutrients in industrial and agricultural wastewater enter closed or semi-closed water bodies in cities, causing serious eutrophication of water bodies and causing algae blooms. Numerous studies have found that Microcystis blooms are the most prevalent among algae blooms. When algae blooms occur, the overgrowth of algae causes other aquatic organisms to die due to hypoxia, and forms secondary metabolites including organic substances, causing the water body to emit different degrees of smell. In addition, some bloom-dominant algae released a large amount of algae toxins into the water body during the metabolic process or after t...

Claims

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Application Information

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IPC IPC(8): C02F1/00C02F1/30
CPCC02F1/00C02F1/30Y02W10/37
Inventor 饶义剑袁振波刘美玲张艳
Owner JIANGNAN UNIV
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