Algistat containing terrestrial plants capable of being cultured in water
An algae inhibitor and plant technology, which is applied in the directions of biocides, plant growth regulators, and chemicals for biological control to achieve the effects of preventing algal blooms, beautifying the environment, and improving algae inhibitory performance.
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Embodiment 1
[0036] The water extract of scallion white is prepared by the following method: leach scallion white and distilled water at room temperature for 4 days, and filter it through a 0.22 μm microporous membrane under sterile conditions to obtain the filtrate; the mass ratio of scallion white and distilled water is 1:10.
[0037] The water extract of the green onion leaves is prepared by the following method: extract the green onion leaves and distilled water at room temperature for 4 days, and filter them through a 0.22 μm microporous membrane under sterile conditions. The mass ratio of the obtained filtrate to the green onion leaves and distilled water is It is 1:10.
Embodiment 2
[0039] The relationship between algae density and fluorescence value.
[0040] Microcystis aeruginosa in the logarithmic growth phase was diluted into 5 algae cell density gradients with BG-11 medium, counted with a hemocytometer, and the fluorescence values were measured respectively.
[0041] The result is as Figure 4 Shown: there is a positive correlation between cell density and its fluorescence value (R2=0.9960), so the fluorescence value can be used to reflect the growth status of Microcystis aeruginosa.
Embodiment 3
[0043] Effects of water extracts of scallion scallion white and scallion leaves at different concentrations on the growth of Microcystis aeruginosa.
[0044] Microcystis aeruginosa in the logarithmic growth phase were put into 100 mL Erlenmeyer flasks, and then BG-11 medium with different dilution factors was added to make the final concentration of medium in each group equal, and the final concentration of algal cells was 5.0×10 5 cells / mL, the final volume of the algae liquid is 50 mL. The final concentrations of water extracts of scallion scallion or scallion leaves in the Erlenmeyer flasks were 0, 10 mL / L, 30 mL / L, and 50 mL / L, respectively, and a control group without adding scallion scallion or scallion leaf water extracts (blank ), each group was set up in 3 parallels, and each group was cultured under the same conditions as in 1.2. Fluorescence values were measured every 24 hours for 5 days.
[0045]Depend on figure 1 It can be seen that each treatment group of t...
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