Bckms21 of burkholderia cepacia and application of Bckms21 of burkholderia cepacia

A Burkholderia and onion technology, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of corn root rot and ear rot that have not been reported, and achieve simple and easy cultivation methods and reduce the number of times of use , good antibacterial effect

Pending Publication Date: 2022-03-04
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no biological seed coating agent prepared with Burkholderia cepacia, and the control of corn root rot and ear rot caused by Fusarium pseudoverticillium has not yet been reported

Method used

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  • Bckms21 of burkholderia cepacia and application of Bckms21 of burkholderia cepacia
  • Bckms21 of burkholderia cepacia and application of Bckms21 of burkholderia cepacia
  • Bckms21 of burkholderia cepacia and application of Bckms21 of burkholderia cepacia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Screening, broad-spectrum, preservation and identification of Burkholderia cepacia Bckms21

[0038] 152 soil samples were randomly collected from 7 provinces of Jilin Province, Inner Mongolia Autonomous Region, Yunnan Province, Heilongjiang Province, Ningxia Hui Autonomous Region, Guizhou Province and Jiangsu Province, and the two-line confrontation method was used to conduct a confrontation test with Fusarium pseudoverticillium. The Burkholderia cepacia Bckms21 has a broad band of inhibition to target bacteria up to 9mm.

[0039] The specific method for screening Burkholderia cepacia Bckms21 is as follows:

[0040] (1) make the soil sample for testing into 10 -2 -10 -6 Wait for 5 gradients of soil suspension, and use the plate coating method to spread evenly on the beef extract peptone medium (NA) plate until it is completely absorbed, set three repetitions, and culture at a constant temperature of 28°C for 24h-48h.

[0041] (2) Pick single colonies with d...

Embodiment 2

[0045] Embodiment 2 measures the antibacterial spectrum of Burkholderia cepacia Bckms21

[0046] In Table 1, corn leaf spot fungus (Exserohilum turcicum), corn leaf spot fungus (Curvularialunata), corn leaf spot fungus (Bipolaris maydis), corn ear rot fungus (Fusarium subglutinans), corn stalk rot fungus (F. graminearum) , corn sheath blight (Rhizoctonia solani), corn sheath rot fungus (F.proliferatum), corn anthracnose fungus (Colletotrichum graminicola), corn root rot fungus (Fusarium verticillioides) as target bacteria, using two-line confrontation method, 28 ℃ constant temperature culture 5d, record the bacteriostatic distance.

[0047] Burkholderia cepacia Bckms21 has a good broad-spectrum inhibitory effect on the above pathogenic bacteria, and the inhibitory distance against P. maize is 17mm ( figure 2 A. figure 2 B), the inhibitory distance to Curvularia maize leaf spot was 14mm (Fig. 2C, figure 2 D), the bacteriostasis distance to corn leaf spot bacterium reaches...

Embodiment 3

[0051] Measure the bacteriostatic rate of Burkholderia cepacia Bckms21 fermented liquid, concrete method is as follows:

[0052] Strain activation: Streak the strain Bckms21 onto a PDA plate and grow in a constant temperature incubator at 28°C for 1-2 days.

[0053] Preparation of Burkholderia cepacia fermentation broth: Pick a single colony of the strain and place it in a 5mL / 50mL LB liquid centrifuge tube, culture it in a shaker at 180r / min at 28°C for 18h, then inoculum with 5% (v / v) Inoculate into 60mL / 250mL Bckms21 fermentation medium Erlenmeyer flask (fermentation medium: glucose 1%, beef extract 1%, potassium chloride 1%, distilled water 1000mL, initial pH=5.27) at 31°C, 200r / min shaker Ferment and cultivate for 48 hours, filter through a 0.22 μL membrane filter, and store in a refrigerator at 4°C for later use.

[0054] Using the growth rate method, the fermentation filtrate of Burkholderia cepacia Bckms21 was mixed with the solid PDA medium cooled to 50°C in a volume...

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Abstract

The invention discloses burkholderia cepacia Bckms21 and application thereof, and belongs to the technical field of plant disease control. The preservation number of the burkholderia cepacia Bckms21 is CGMCC (China General Microbiological Culture Collection Center) NO. 21693. The burkholderia cepacia Bckms21 has broad-spectrum antibacterial ability, and also has siderophore producing ability and phosphate solubilizing ability; meanwhile, the invention provides a method for preparing fermentation liquor of the burkholderia cepacia Bckms21, a biological seed coating agent coating method and application of the biological seed coating agent, the biological seed coating agent is convenient to prepare, easy to coat, efficient, low in toxicity and relatively safe to the environment, and has a remarkable growth promoting effect on bud length and root length during corn seed germination; the composition has good prevention and treatment effects on fusarium root rot, stem rot and spike rot of corn.

Description

technical field [0001] The invention relates to Burkholderia cepacia Bckms21 and an application thereof, belonging to the technical field of plant disease prevention and control. Background technique [0002] Corn is the food crop with the largest planting area and total output in my country. In 2021, the national sowing area will be about 650 million mu, and the annual output will be about 273 million tons. In recent years, with the popularization and application of cultivation measures such as no-tillage and straw returning to the field, the pathogenic bacteria populations that cause soil-borne diseases of corn in the soil have increased significantly. Grass disease, stem rot and ear rot occur seriously, the incidence rate is 5-10% in normal years, and the incidence rate is more than 40% in severe years, causing serious yield loss, and has become the main threat to high-quality and high-yield corn in my country. [0003] The methods of preventing Fusarium corn disease mai...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P3/00A01P21/00A01C1/06A01C1/08C12R1/01
CPCC12N1/20A01N63/20A01C1/06A01C1/08
Inventor 肖淑芹吴跃星李玉蕾薛春生崔金月金宝
Owner SHENYANG AGRI UNIV
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