Preparation method and application of shewanella embedded gel fiber

A Shewanella and gel fiber technology, applied in the field of pollution control and remediation, can solve problems such as application limitation, insufficient greening of degradable materials, etc., achieve good biocompatibility, improve spinnability and mechanical properties, Simple to use effects

Active Publication Date: 2022-03-01
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have solved the problem of the adhesion of materials and the survival of bacterial agents, the optimization of the environment for microbial growth, the catalysis of the degradation process, and the greening of degraded materials are still insufficient, and their applications are limited.

Method used

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  • Preparation method and application of shewanella embedded gel fiber
  • Preparation method and application of shewanella embedded gel fiber

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 The present invention is used to catalyze the degradation of antibiotics, the gel fiber embedded with Shewanella, the preparation method is as follows:

[0034] (1) prepare the mixed solution of polyvinyl alcohol and sodium alginate, wherein with the total reaction volume as a reference, the dosage of sodium alginate is 4% (w / w), and the dosage of polyvinyl alcohol is 25% (w / w) / w), stirring for 1 h under heating in a water bath at 80°C to make it completely mixed;

[0035] (2) Adjust the pH of the suspension described in (1) to 8.0 (±0.1) with NaOH and HCl at a concentration of 1 mol / L;

[0036] (3) Cross-linking: adding FeCl with the concentration of 0.15mol / L equal to the volume of (1) to the suspension described in (2) 3 Aqueous solution, stirred at 50°C for 3h;

[0037] (4) Electrospinning the suspension described in (3) to obtain sodium alginate / polyvinyl alcohol composite fibers

[0038] (5) Preparation of Shewanella bacterium liquid: Select Shewanel...

Embodiment 2

[0045] (1) prepare the mixed solution of polyvinyl alcohol and sodium alginate, wherein with the total reaction volume as a reference, the dosage of sodium alginate is 1% (w / w), and the dosage of polyvinyl alcohol is 20% (w / w) / w), stirring for 1 h under heating in a water bath at 80°C to make it completely mixed;

[0046] (2) Adjust the pH of the suspension described in (1) to 8.0 (±0.1) with NaOH and HCl at a concentration of 1 mol / L;

[0047] (3) Cross-linking: adding FeCl with the concentration of 0.1mol / L equal to the volume of (1) to the suspension described in (2) 3 Aqueous solution, stirred at 50°C for 2h;

[0048] (4) Electrospinning the suspension described in (3) to obtain sodium alginate / polyvinyl alcohol composite fibers

[0049] (5) Preparation of Shewanella bacterium liquid: Select Shewanella oneidensis MR-1 and place it in LB liquid medium for 18 hours on a constant temperature shaker at 30°C and 180r / min until the cell density is 1×10 8 a / mL;

[0050] (6) ...

Embodiment 3

[0056] (1) prepare the mixed solution of polyvinyl alcohol and sodium alginate, wherein with the total reaction volume as a reference, the dosage of sodium alginate is 5% (w / w), and the dosage of polyvinyl alcohol is 30% (w / w) / w), stirring for 2 hours under heating in a water bath at 80°C to make it completely mixed;

[0057] (2) Adjust the pH of the suspension described in (1) to 8.0 (±0.1) with NaOH and HCl at a concentration of 1 mol / L;

[0058] (3) Cross-linking: adding FeCl with a concentration equal to that of (1) to the suspension described in (2) is 0.5 mol / L 3 Aqueous solution, stirred at 50°C for 2h;

[0059] (4) Electrospinning the suspension described in (3) to obtain sodium alginate / polyvinyl alcohol composite fibers

[0060] (5) Preparation of Shewanella bacterium liquid: Select Shewanella oneidensis MR-1 and place it in LB liquid medium for 18 hours on a constant temperature shaker at 30°C and 180r / min until the cell density is 1×10 8 a / mL;

[0061] (6) Soa...

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Abstract

The invention belongs to the field of pollution control and remediation, and discloses a preparation method and application of shewanella embedded gel fiber in order to solve the problems of insufficient material function effect and limited application in the existing method and form an efficient antibiotic pollution remediation agent which is simple to operate and environment-friendly. The method comprises the following steps: mixing polyvinyl alcohol and sodium alginate to form a suspension, adjusting the pH value, adding FeCl3 for crosslinking, and then carrying out electrostatic spinning to obtain sodium alginate / polyvinyl alcohol composite fibers; and soaking the fiber in a shewanella bacterial suspension, drying, soaking in a gelatin aqueous solution, and drying again to obtain the gel fiber embedded with shewanella. The raw materials are easy to obtain and biodegradable, the gel fiber is simple in preparation method, high in plasticity and environment-friendly, the operation of repairing antibiotic pollution by using the fiber is simple, and the fiber can be directly applied to in-situ repair and can be prepared and popularized in a large scale.

Description

technical field [0001] The invention belongs to the field of pollution control and restoration, and in particular relates to a preparation method and application of a gel fiber embedded with Shewanella bacteria. Background technique [0002] Antibiotics have been used worldwide since the introduction of penicillin in 1929. A large number of antibiotic residues in the environment will induce the increase of bacterial resistance and the generation and spread of resistance genes (ARGs), which will cause potential adverse effects on the ecological environment and human health. [0003] Biodegradation can degrade antibiotics through the metabolism of microorganisms. The degradation process is relatively safe and less expensive than other methods. The core link is to ensure the survival and activity of microorganisms in antibiotic-contaminated environments. At present, immobilized microorganism reduction technology is gradually emerging. , this method has two key links, one is to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D06M15/15D06M16/00D01F8/18D01F8/10C12N11/10C12N11/084C02F3/34C12R1/01C02F101/38D06M101/04D06M101/24
CPCD06M15/15D06M16/003D01F8/18D01F8/10C12N11/10C12N11/084C02F3/34C02F2101/38C02F2101/40D06M2101/04D06M2101/24
Inventor 朱宇恩李华李媛媛
Owner SHANXI UNIV
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