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Preparation method of photo-cured 3D printing bio-compatible silk fibroin hydrogel

A 3D printing and silk fibroin technology, applied in the direction of additive processing, etc., can solve the problems of low preparation speed and low precision, and achieve the effects of adjustable degradability, good biocompatibility, and wide sources

Pending Publication Date: 2022-02-22
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Combining 3D printing technology to solve the problems of low preparation speed and low precision in the existing technology

Method used

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  • Preparation method of photo-cured 3D printing bio-compatible silk fibroin hydrogel
  • Preparation method of photo-cured 3D printing bio-compatible silk fibroin hydrogel
  • Preparation method of photo-cured 3D printing bio-compatible silk fibroin hydrogel

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Experimental program
Comparison scheme
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Embodiment 1

[0045] A preparation method of photocuring 3D printing biocompatible silk fibroin hydrogel provided in this embodiment comprises the following steps:

[0046] S1. Preparation of printing materials;

[0047] S11, preparation of silk fibroin (SF): silkworm cocoons were boiled in mass concentration of 0.3% w / v sodium oleate and 0.2% w / v sodium carbonate solution for 1 hour to remove sericin, then washed and dried at 80 Dissolve 10g of degummed cocoons in 50ml of 9.3mol / l lithium bromide solution at ℃ for 30 minutes to obtain SF solution, then add 10ml of 0.6mol / l sodium oxide solution directly to the SF solution and hydrolyze at 80℃ for 12h, then use acetic acid Cellulose dialysis tubing was dialyzed against deionized water for 3 days. After dialysis, the SF solution was centrifuged at 15,000 g for 20 minutes to remove insoluble aggregates, and then lyophilized;

[0048] S12, preparation of hyaluronic acid-methacrylate (HAMA): dissolve hyaluronic acid (HA) in deionized water to ...

Embodiment 2

[0053] A preparation method of photocuring 3D printing biocompatible silk fibroin hydrogel provided in this embodiment comprises the following steps:

[0054] S1. Preparation of printing materials;

[0055] S11. Preparation of silk fibroin (SF): Silkworm cocoons were boiled in 0.3% w / v sodium oleate and 0.2% w / v sodium carbonate solution for 1 hour to remove sericin, then washed and dried. 10 g of degummed cocoons were dissolved in 50 ml of 9.3 mol / l lithium bromide solution at 80° C. for 30 minutes. Subsequently, 10 ml of a 0.6 mol / l sodium oxide solution were directly added to the SF solution. Hydrolysis was carried out at 80° C. for 12 h, followed by dialysis against deionized water for 3 days using cellulose acetate dialysis tubing. After dialysis, the SF solution was centrifuged at 15,000g for 20 minutes to remove insoluble aggregates and then lyophilized;

[0056] S12. Preparation of hyaluronic acid-methacrylate (HAMA): Hyaluronic acid (HA) was dissolved in deionized ...

Embodiment 3

[0061] A preparation method of photocuring 3D printing biocompatible silk fibroin hydrogel provided in this embodiment comprises the following steps:

[0062] S1. Preparation of printing materials;

[0063] S11. Preparation of silk fibroin (SF): Silkworm cocoons were boiled in 0.3% w / v sodium oleate and 0.2% w / v sodium carbonate solution for 1 hour to remove sericin, then washed and dried. 10 g of degummed cocoons were dissolved in 50 ml of 9.3 mol / l lithium bromide solution at 80° C. for 30 minutes. Subsequently, 10 ml of a 0.6 mol / l sodium oxide solution were directly added to the SF solution. Hydrolysis was carried out at 80° C. for 12 h, followed by dialysis against deionized water for 3 days using cellulose acetate dialysis tubing. After dialysis, the SF solution was centrifuged at 15,000g for 20 minutes to remove insoluble aggregates and then lyophilized;

[0064] S12. Preparation of hyaluronic acid-methacrylate (HAMA): Hyaluronic acid (HA) was dissolved in deionized ...

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Abstract

The invention discloses a preparation method of photo-cured 3D printing bio-compatible silk fibroin hydrogel. The preparation method comprises the following steps: taking hyaluronic acid and silk fibroin as raw materials, modifying the hyaluronic acid and the silk fibroin to respectively obtain hyaluronic acid-methacrylate and silk fibroin-methacrylate, mixing a hyaluronic acid-methacrylate solution and a silk fibroin-methacrylate solution according to a certain proportion to obtain a precursor solution, and constructing the hydrogel through a photo curing 3D printing technology. The degradability of the hydrogel is improved through treatment of a methanol solution or an ethanol solution. According to the invention, the excellent characteristics of silk fibroin and hyaluronic acid are combined, and the hydrogel has good bio-compatibility and adjustable degradability by controlling the mixing ratio of hyaluronic acid-methacrylate and silk fibroin-methacrylate prepolymer solutions and treating the hydrogel with methanol or ethanol.

Description

technical field [0001] The invention belongs to the field of hydrogel technology, and in particular relates to a preparation method of photocuring 3D printing biocompatible silk fibroin hydrogel. Background technique [0002] Hyaluronic acid (HA) is widely used in biomedicine because of its excellent biocompatibility and biosafety. Furthermore, HA hydrogels, which form HA networks through covalent crosslinking, are frequently used not only in plastic surgery but also in tissue engineering. Nevertheless, conventional HA hydrogels still have some limitations in further applications, the control of physicochemical properties of HA hydrogels is limited by pure HA molecules, low cell adhesion occurs, viscoelastic properties and HA hydrogels Fatal flaws such as rapid degradation. [0003] Silk fibroin (SF) is a fibrous protein obtained from silkworm cocoons. So far, many studies have demonstrated the excellent performance of SF-based materials in the biomedical field. SF and S...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08J3/24C08L89/00C08L5/08B33Y70/00
CPCC08J3/075C08J3/246B33Y70/00C08J2389/00C08J2305/08C08J2489/00
Inventor 王莉王正铎罗钰李文张林涛贾希蓓张皓李莹涛
Owner XI AN JIAOTONG UNIV
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